Evidence for enzymatic activity in the absence of solvent in gas-phase complexes of lysozyme and oligosaccharides

Fei He, Javier Ramirez, Carlito B Lebrilla

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hen egg-white lysozyme complexed to substrate and nonsubstrate oligosaccharides are examined by electrospray ionization mass spectrometry. Heated capillary dissociation and collision induced dissociation (CID) are used to characterize the complexes. The relative order of stability obtained in gas phase agrees well with their solution-phase association constants. A proton transfer reaction occurs during the CID of substrate complexes that is not observed with non-substrate complexes. Oligosaccharide fragments are also observed with lysozyme-chitohexaose complex during CD. The possibility of lysozyme activity in the gas phase, or a gas-phase enzymatic activity, is explored. (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)103-114
Number of pages12
JournalInternational Journal of Mass Spectrometry
Volume193
Issue number2-3
DOIs
StatePublished - Dec 20 1999

Fingerprint

Oligosaccharides
lysozyme
Muramidase
Enzymes
Gases
dissociation
vapor phases
Thermodynamic properties
Electrospray ionization
Proton transfer
collisions
eggs
Substrates
Mass spectrometry
mass spectroscopy
fragments
Association reactions
ionization
protons

Keywords

  • CID
  • Enzyme
  • Gas phase
  • Lysozyme

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy

Cite this

Evidence for enzymatic activity in the absence of solvent in gas-phase complexes of lysozyme and oligosaccharides. / He, Fei; Ramirez, Javier; Lebrilla, Carlito B.

In: International Journal of Mass Spectrometry, Vol. 193, No. 2-3, 20.12.1999, p. 103-114.

Research output: Contribution to journalArticle

@article{f013aec562244d7698772d9fe4df883d,
title = "Evidence for enzymatic activity in the absence of solvent in gas-phase complexes of lysozyme and oligosaccharides",
abstract = "Hen egg-white lysozyme complexed to substrate and nonsubstrate oligosaccharides are examined by electrospray ionization mass spectrometry. Heated capillary dissociation and collision induced dissociation (CID) are used to characterize the complexes. The relative order of stability obtained in gas phase agrees well with their solution-phase association constants. A proton transfer reaction occurs during the CID of substrate complexes that is not observed with non-substrate complexes. Oligosaccharide fragments are also observed with lysozyme-chitohexaose complex during CD. The possibility of lysozyme activity in the gas phase, or a gas-phase enzymatic activity, is explored. (C) 1999 Elsevier Science B.V.",
keywords = "CID, Enzyme, Gas phase, Lysozyme",
author = "Fei He and Javier Ramirez and Lebrilla, {Carlito B}",
year = "1999",
month = "12",
day = "20",
doi = "10.1016/S1387-3806(99)00167-0",
language = "English (US)",
volume = "193",
pages = "103--114",
journal = "International Journal of Mass Spectrometry",
issn = "1387-3806",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Evidence for enzymatic activity in the absence of solvent in gas-phase complexes of lysozyme and oligosaccharides

AU - He, Fei

AU - Ramirez, Javier

AU - Lebrilla, Carlito B

PY - 1999/12/20

Y1 - 1999/12/20

N2 - Hen egg-white lysozyme complexed to substrate and nonsubstrate oligosaccharides are examined by electrospray ionization mass spectrometry. Heated capillary dissociation and collision induced dissociation (CID) are used to characterize the complexes. The relative order of stability obtained in gas phase agrees well with their solution-phase association constants. A proton transfer reaction occurs during the CID of substrate complexes that is not observed with non-substrate complexes. Oligosaccharide fragments are also observed with lysozyme-chitohexaose complex during CD. The possibility of lysozyme activity in the gas phase, or a gas-phase enzymatic activity, is explored. (C) 1999 Elsevier Science B.V.

AB - Hen egg-white lysozyme complexed to substrate and nonsubstrate oligosaccharides are examined by electrospray ionization mass spectrometry. Heated capillary dissociation and collision induced dissociation (CID) are used to characterize the complexes. The relative order of stability obtained in gas phase agrees well with their solution-phase association constants. A proton transfer reaction occurs during the CID of substrate complexes that is not observed with non-substrate complexes. Oligosaccharide fragments are also observed with lysozyme-chitohexaose complex during CD. The possibility of lysozyme activity in the gas phase, or a gas-phase enzymatic activity, is explored. (C) 1999 Elsevier Science B.V.

KW - CID

KW - Enzyme

KW - Gas phase

KW - Lysozyme

UR - http://www.scopus.com/inward/record.url?scp=0033590021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033590021&partnerID=8YFLogxK

U2 - 10.1016/S1387-3806(99)00167-0

DO - 10.1016/S1387-3806(99)00167-0

M3 - Article

VL - 193

SP - 103

EP - 114

JO - International Journal of Mass Spectrometry

JF - International Journal of Mass Spectrometry

SN - 1387-3806

IS - 2-3

ER -