Abstract
Hen egg-white lysozyme complexed to substrate and nonsubstrate oligosaccharides are examined by electrospray ionization mass spectrometry. Heated capillary dissociation and collision induced dissociation (CID) are used to characterize the complexes. The relative order of stability obtained in gas phase agrees well with their solution-phase association constants. A proton transfer reaction occurs during the CID of substrate complexes that is not observed with non-substrate complexes. Oligosaccharide fragments are also observed with lysozyme-chitohexaose complex during CD. The possibility of lysozyme activity in the gas phase, or a gas-phase enzymatic activity, is explored. (C) 1999 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 103-114 |
| Number of pages | 12 |
| Journal | International Journal of Mass Spectrometry |
| Volume | 193 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Dec 20 1999 |
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Keywords
- CID
- Enzyme
- Gas phase
- Lysozyme
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Spectroscopy
Cite this
Evidence for enzymatic activity in the absence of solvent in gas-phase complexes of lysozyme and oligosaccharides. / He, Fei; Ramirez, Javier; Lebrilla, Carlito B.
In: International Journal of Mass Spectrometry, Vol. 193, No. 2-3, 20.12.1999, p. 103-114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence for enzymatic activity in the absence of solvent in gas-phase complexes of lysozyme and oligosaccharides
AU - He, Fei
AU - Ramirez, Javier
AU - Lebrilla, Carlito B
PY - 1999/12/20
Y1 - 1999/12/20
N2 - Hen egg-white lysozyme complexed to substrate and nonsubstrate oligosaccharides are examined by electrospray ionization mass spectrometry. Heated capillary dissociation and collision induced dissociation (CID) are used to characterize the complexes. The relative order of stability obtained in gas phase agrees well with their solution-phase association constants. A proton transfer reaction occurs during the CID of substrate complexes that is not observed with non-substrate complexes. Oligosaccharide fragments are also observed with lysozyme-chitohexaose complex during CD. The possibility of lysozyme activity in the gas phase, or a gas-phase enzymatic activity, is explored. (C) 1999 Elsevier Science B.V.
AB - Hen egg-white lysozyme complexed to substrate and nonsubstrate oligosaccharides are examined by electrospray ionization mass spectrometry. Heated capillary dissociation and collision induced dissociation (CID) are used to characterize the complexes. The relative order of stability obtained in gas phase agrees well with their solution-phase association constants. A proton transfer reaction occurs during the CID of substrate complexes that is not observed with non-substrate complexes. Oligosaccharide fragments are also observed with lysozyme-chitohexaose complex during CD. The possibility of lysozyme activity in the gas phase, or a gas-phase enzymatic activity, is explored. (C) 1999 Elsevier Science B.V.
KW - CID
KW - Enzyme
KW - Gas phase
KW - Lysozyme
UR - http://www.scopus.com/inward/record.url?scp=0033590021&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033590021&partnerID=8YFLogxK
U2 - 10.1016/S1387-3806(99)00167-0
DO - 10.1016/S1387-3806(99)00167-0
M3 - Article
VL - 193
SP - 103
EP - 114
JO - International Journal of Mass Spectrometry
JF - International Journal of Mass Spectrometry
SN - 1387-3806
IS - 2-3
ER -