Evidence for cardiac sodium-calcium exchanger association with caveolin-3

Julie B C Bossuyt, Bonnie E. Taylor, Marilyn James-Kracke, Calvin C. Hale

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The interaction of cardiac Na+-Ca2+ exchange (NCX1) with caveolin proteins was investigated in sarcolemmal vesicles. Western blots of sarcolemmal vesicles revealed the presence of caveolin-1, -2, and -3. NCX1 co-fractionated more closely with caveolin-3 than caveolin-1 on sucrose density gradients. NCX1 has five possible caveolin-binding motifs and NCX1 co-precipitated specifically with caveolin-3. Molecular sieve column chromatography indicated that this co-precipitation was not due to incomplete solubilization of lipid raft microdomains. Cholesterol chelation in vesicles decreased NCX1 transport activity and caveolin-3 co-precipitation. NCX1 may play a role in caveolar transmembrane signaling in addition to its role in excitation-contraction coupling.

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalFEBS Letters
Volume511
Issue number1-3
DOIs
StatePublished - Jan 30 2002
Externally publishedYes

Fingerprint

Caveolin 3
Sodium-Calcium Exchanger
Caveolins
Caveolin 1
Association reactions
Coprecipitation
Caveolin 2
Excitation Contraction Coupling
Column chromatography
Molecular sieves
Chelation
Gel Chromatography
Sucrose
Western Blotting
Cholesterol
Lipids

Keywords

  • Cardiac Na-Ca exchange
  • Caveola
  • Sarcolemma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Evidence for cardiac sodium-calcium exchanger association with caveolin-3. / Bossuyt, Julie B C; Taylor, Bonnie E.; James-Kracke, Marilyn; Hale, Calvin C.

In: FEBS Letters, Vol. 511, No. 1-3, 30.01.2002, p. 113-117.

Research output: Contribution to journalArticle

Bossuyt, Julie B C ; Taylor, Bonnie E. ; James-Kracke, Marilyn ; Hale, Calvin C. / Evidence for cardiac sodium-calcium exchanger association with caveolin-3. In: FEBS Letters. 2002 ; Vol. 511, No. 1-3. pp. 113-117.
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