Evidence for cardiac sodium-calcium exchanger association with caveolin-3

Julie B C Bossuyt, Bonnie E. Taylor, Marilyn James-Kracke, Calvin C. Hale

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

The interaction of cardiac Na+-Ca2+ exchange (NCX1) with caveolin proteins was investigated in sarcolemmal vesicles. Western blots of sarcolemmal vesicles revealed the presence of caveolin-1, -2, and -3. NCX1 co-fractionated more closely with caveolin-3 than caveolin-1 on sucrose density gradients. NCX1 has five possible caveolin-binding motifs and NCX1 co-precipitated specifically with caveolin-3. Molecular sieve column chromatography indicated that this co-precipitation was not due to incomplete solubilization of lipid raft microdomains. Cholesterol chelation in vesicles decreased NCX1 transport activity and caveolin-3 co-precipitation. NCX1 may play a role in caveolar transmembrane signaling in addition to its role in excitation-contraction coupling.

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalFEBS Letters
Volume511
Issue number1-3
DOIs
StatePublished - Jan 30 2002
Externally publishedYes

Keywords

  • Cardiac Na-Ca exchange
  • Caveola
  • Sarcolemma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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