Evidence for an early immunosuppressive role for related Campoletis sonorensis venom and ovarian proteins in Heliothis virescens.

B. A. Webb, S. Luckhart

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

Shared epitopes among venom, ovarian, and viral proteins may indicate that related proteins have similar functional roles during parasitization of Heliothis virescens by Campoletis sonorensis. Venom and ovarian proteins are introduced directly into the hemolymph during parasitization where they may target hemocytes or other components of the immune system. Polydnavirus expression has been detected in hemocytes, fat body, and other tissues but has not been detected earlier than 4 h after parasitization. Therefore, effects on hemocytes at times earlier than 4 h may not be caused by polydnavirus proteins synthesized in the parasitized insect. Visualization of hemocyte F-actin with fluorescently labeled phallicidin indicated that a dramatic alteration of plasmatocyte and granulocyte cytoskeletons occurred within 1.5 h after parasitization. The predominant non-viral proteins in the ovary introduced during parasitization were immunologically related to venom and viral envelope proteins. These ovarian proteins persist in the hemolymph. Antisera to the ovarian proteins bound to granulocytes and to plasmatocytes to a lesser degree, suggesting that ovarian proteins may be involved in early suppression of the host's immune response after parasitization.

Original languageEnglish (US)
Pages (from-to)147-163
Number of pages17
JournalArchives of Insect Biochemistry and Physiology
Volume26
Issue number2-3
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Evidence for an early immunosuppressive role for related Campoletis sonorensis venom and ovarian proteins in Heliothis virescens.'. Together they form a unique fingerprint.

  • Cite this