Evidence for a two membrane-spanning autonomous mitochondrial DNA replisome

Shelly Meeusen, Jodi Nunnari

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

The unit of inheritance for mitochondrial DNA (mtDNA) is a complex nucleoprotein structure termed the nucleoid. The organization of the nucleoid as well as its role in mtDNA replication remain largely unknown. Here, we show in Saccharomyces cerevisiae that at least two populations of nucleoids exist within the same mitochondrion and can be distinguished by their association with a discrete proteinaceous structure that spans the outer and inner mitochondrial membranes. Surprisingly, this two membrane-spanning structure (TMS) persists and self-replicates in the absence of mtDNA. We tested whether TMS functions to direct the replication of mtDNA. By monitoring BrdU incorporation, we observed that actively replicating nucleoids are associated exclusively with TMS. Consistent with TMS's role in mtDNA replication, we found that Mip1, the mtDNA polymerase, is also a stable component of TMS. Taken together, our observations reveal the existence of an autonomous two membrane-spanning mitochondrial replisome as well as provide a mechanism for how mtDNA replication and inheritance may be physically linked.

Original languageEnglish (US)
Pages (from-to)503-510
Number of pages8
JournalJournal of Cell Biology
Volume163
Issue number3
DOIs
StatePublished - Nov 10 2003

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Mitochondrial DNA
Membranes
Mitochondrial Membranes
DNA Replication
Nucleoproteins
Mitochondrial Genes
Bromodeoxyuridine
DNA-Directed DNA Polymerase
DNA synthesome
Saccharomyces cerevisiae
Mitochondria
Population

Keywords

  • Membrane-spanning
  • Mitochondria
  • mtDNA
  • Nucleoid
  • Replisome

ASJC Scopus subject areas

  • Cell Biology

Cite this

Evidence for a two membrane-spanning autonomous mitochondrial DNA replisome. / Meeusen, Shelly; Nunnari, Jodi.

In: Journal of Cell Biology, Vol. 163, No. 3, 10.11.2003, p. 503-510.

Research output: Contribution to journalArticle

Meeusen, Shelly ; Nunnari, Jodi. / Evidence for a two membrane-spanning autonomous mitochondrial DNA replisome. In: Journal of Cell Biology. 2003 ; Vol. 163, No. 3. pp. 503-510.
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