Evidence for a stem cell common to hematopoiesis and its in vitro microenvironment: Studies of patients with clonal hematopoietic neoplasia

Jack W. Singer, Armand Keating, Janet Cuttner, Allen M. Gown, Robert Jacobson, Paul D. Killen, John W. Moohr, Vesna Najfeld, Jerry S Powell, Jean Sanders, Gary E. Striker, Philip J. Fialkow

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

The origin and nature of cells forming the in vitro microenvironment in long-term cultures of human marrow were studied in five patients with clonal myeloproliferative disorders who were heterozygous for glucose-6-phosphatase dehydrogenase (G6PD). The results showed that cells in the adherent stromal layer forming the in vitro microenvironment were derived from the same clonal progenitors involved by the neoplasm in the four patients whose diseases originated in multipotent stem cells. In contrast, stromal cells were derived from normal progenitors in a patient with acute non-lymphocytic leukemia whose clone showed differentiative expression confined to cells in the granulocytic lineage. Mixing experiments demonstrated that the G6PD type displayed by the adherent marrow stromal cells was not obscured by contaminating non-adherent hematopoietic cells or marrow fibroblasts. The data suggest the existence of a pluripotent cell in normal hematopoiesis that gives rise to hematopoietic cells and to their microenvironment.

Original languageEnglish (US)
Pages (from-to)535-545
Number of pages11
JournalLeukemia Research
Volume8
Issue number4
DOIs
StatePublished - 1984
Externally publishedYes

Keywords

  • acute non-lymphocytic leukemia
  • Chronic myelogenous leukemia
  • collagen synthesis
  • glucose-6-phosphate dehydrogenase
  • hematopoietic microenvironment
  • long-term marrow culture
  • stem cell

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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  • Cite this

    Singer, J. W., Keating, A., Cuttner, J., Gown, A. M., Jacobson, R., Killen, P. D., Moohr, J. W., Najfeld, V., Powell, J. S., Sanders, J., Striker, G. E., & Fialkow, P. J. (1984). Evidence for a stem cell common to hematopoiesis and its in vitro microenvironment: Studies of patients with clonal hematopoietic neoplasia. Leukemia Research, 8(4), 535-545. https://doi.org/10.1016/0145-2126(84)90002-X