Evidence for a physiological role for membrane rafts in human platelets

Karine Gousset, Willem F. Wolkers, Nelly M. Tsvetkova, Ann E. Oliver, Cara L. Field, Naomi J. Walker, John H. Crowe, Fern Tablin

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

We have investigated raft formation in human platelets in response to cell activation. Lipid phase separation and domain formation were detected using the fluorescent dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate (dil-C18) that preferentially partitions into gel-like lipid domains. We showed that when human platelets are activated by cold and physiological agonists, rafts coalesce into visible aggregates. These events were disrupted by depletion of membrane cholesterol. Using Fourier transform infrared spectroscopy (FTIR), we measured a thermal phase transition at around 30°C in intact platelets, which we have assigned as the liquid-ordered to the liquid-disordered phase transition of rafts. Phase separation of the phospholipid and the sphingomyelin-enriched rafts could be observed as two phase transitions at around 15 and 30°C, respectively. The higher transition, assigned to the rafts, was greatly enhanced with removal of membrane cholesterol. Detergent-resistant membranes (DRMs) were enriched in cholesterol (50%) and sphingomyelin (20%). The multi-functional platelet receptor CD36 selectively partitioned into DRMs, whereas the GPI-linked protein CD55 and the major platelet integrin α11bβ3a did not, which suggests that the clustering of proteins within rafts is a regulated process dependent on specific lipid protein interactions. We suggest that raft aggregation is a dynamic, reversible physiological event triggered by cell activation.

Original languageEnglish (US)
Pages (from-to)117-128
Number of pages12
JournalJournal of Cellular Physiology
Volume190
Issue number1
DOIs
StatePublished - 2002

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Platelets
Blood Platelets
Phase Transition
Membranes
Sphingomyelins
Phase transitions
Cholesterol
Lipids
Phase separation
Detergents
GPI-Linked Proteins
Chemical activation
Liquids
Fourier Transform Infrared Spectroscopy
Fluorescent Dyes
Integrins
Cluster Analysis
Phospholipids
Proteins
Agglomeration

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Gousset, K., Wolkers, W. F., Tsvetkova, N. M., Oliver, A. E., Field, C. L., Walker, N. J., ... Tablin, F. (2002). Evidence for a physiological role for membrane rafts in human platelets. Journal of Cellular Physiology, 190(1), 117-128. https://doi.org/10.1002/jcp.10039

Evidence for a physiological role for membrane rafts in human platelets. / Gousset, Karine; Wolkers, Willem F.; Tsvetkova, Nelly M.; Oliver, Ann E.; Field, Cara L.; Walker, Naomi J.; Crowe, John H.; Tablin, Fern.

In: Journal of Cellular Physiology, Vol. 190, No. 1, 2002, p. 117-128.

Research output: Contribution to journalArticle

Gousset, K, Wolkers, WF, Tsvetkova, NM, Oliver, AE, Field, CL, Walker, NJ, Crowe, JH & Tablin, F 2002, 'Evidence for a physiological role for membrane rafts in human platelets', Journal of Cellular Physiology, vol. 190, no. 1, pp. 117-128. https://doi.org/10.1002/jcp.10039
Gousset K, Wolkers WF, Tsvetkova NM, Oliver AE, Field CL, Walker NJ et al. Evidence for a physiological role for membrane rafts in human platelets. Journal of Cellular Physiology. 2002;190(1):117-128. https://doi.org/10.1002/jcp.10039
Gousset, Karine ; Wolkers, Willem F. ; Tsvetkova, Nelly M. ; Oliver, Ann E. ; Field, Cara L. ; Walker, Naomi J. ; Crowe, John H. ; Tablin, Fern. / Evidence for a physiological role for membrane rafts in human platelets. In: Journal of Cellular Physiology. 2002 ; Vol. 190, No. 1. pp. 117-128.
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