Evidence for a contribution of I(Ca) to serotonergic modulation of I(K,Ca) in Hermissenda photoreceptors

E. N. Yamoah, T. Crow

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

1. The Ca2+-dependent K+ current (I(K,Ca)) contributes to both the plateau phase of light-elicited generator potentials and enhanced excitability of identified type B photoreceptors of Hermissenda detected after classical conditioning. Serotonergic modulation of membrane conductances mimics some of the effects of conditioning. Serotonin (5-HT) reduces the magnitude of I(K,Ca) and decreases the sustained voltage- activated Ca2+ current (I(Ca)) in type B photoreceptors. We have examined the modulatory role of 5-HT in regulation of I(K,Ca) by I(C) using a Ca2+ ionophore in conjunction with the whole cell patch-clamp technique in isolated photoreceptors. 2. The 40-50% reduction of I(K,Ca) by 5-HT was voltage independent. Cd2+ blocked I(Ca) and reduced I(K,Ca) by 70-80%. The remaining 20-30% of I(K,Ca) may result from Ca2+ release from intracellular stores, because I(K,Ca) was further reduced to 5-10% as the pipette ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) was raised from 0.5 to 5 mM. The application of the Ca2+ ionophore A23187, which was designed to produce Ca2+ influx independent of the voltage-activated Ca2+ channels, restored I(K,Ca). 3. The application of A23187 reversed the effects of 5-HT and Cd2+ on I(K,Ca) for experiments lasting 15-20 min. However, for longer time periods (>25 min), complete restoration of I(K,Ca) by A23187 was obtained in the presence of Cd2+ but not 5-HT. These results suggest that for 15 to 20 min exposures the reduction of I(K,Ca) by 5-HT is a consequence of modulation of I(Ca) by 5-HT and not a direct effect of 5-HT on I(K,Ca).

Original languageEnglish (US)
Pages (from-to)1349-1354
Number of pages6
JournalJournal of Neurophysiology
Volume74
Issue number3
StatePublished - 1995
Externally publishedYes

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Hermissenda
Serotonin
Calcimycin
Ionophores
Classical Conditioning
Ethylene Glycol
Egtazic Acid
Patch-Clamp Techniques
Ether

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Evidence for a contribution of I(Ca) to serotonergic modulation of I(K,Ca) in Hermissenda photoreceptors. / Yamoah, E. N.; Crow, T.

In: Journal of Neurophysiology, Vol. 74, No. 3, 1995, p. 1349-1354.

Research output: Contribution to journalArticle

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title = "Evidence for a contribution of I(Ca) to serotonergic modulation of I(K,Ca) in Hermissenda photoreceptors",
abstract = "1. The Ca2+-dependent K+ current (I(K,Ca)) contributes to both the plateau phase of light-elicited generator potentials and enhanced excitability of identified type B photoreceptors of Hermissenda detected after classical conditioning. Serotonergic modulation of membrane conductances mimics some of the effects of conditioning. Serotonin (5-HT) reduces the magnitude of I(K,Ca) and decreases the sustained voltage- activated Ca2+ current (I(Ca)) in type B photoreceptors. We have examined the modulatory role of 5-HT in regulation of I(K,Ca) by I(C) using a Ca2+ ionophore in conjunction with the whole cell patch-clamp technique in isolated photoreceptors. 2. The 40-50{\%} reduction of I(K,Ca) by 5-HT was voltage independent. Cd2+ blocked I(Ca) and reduced I(K,Ca) by 70-80{\%}. The remaining 20-30{\%} of I(K,Ca) may result from Ca2+ release from intracellular stores, because I(K,Ca) was further reduced to 5-10{\%} as the pipette ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) was raised from 0.5 to 5 mM. The application of the Ca2+ ionophore A23187, which was designed to produce Ca2+ influx independent of the voltage-activated Ca2+ channels, restored I(K,Ca). 3. The application of A23187 reversed the effects of 5-HT and Cd2+ on I(K,Ca) for experiments lasting 15-20 min. However, for longer time periods (>25 min), complete restoration of I(K,Ca) by A23187 was obtained in the presence of Cd2+ but not 5-HT. These results suggest that for 15 to 20 min exposures the reduction of I(K,Ca) by 5-HT is a consequence of modulation of I(Ca) by 5-HT and not a direct effect of 5-HT on I(K,Ca).",
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N2 - 1. The Ca2+-dependent K+ current (I(K,Ca)) contributes to both the plateau phase of light-elicited generator potentials and enhanced excitability of identified type B photoreceptors of Hermissenda detected after classical conditioning. Serotonergic modulation of membrane conductances mimics some of the effects of conditioning. Serotonin (5-HT) reduces the magnitude of I(K,Ca) and decreases the sustained voltage- activated Ca2+ current (I(Ca)) in type B photoreceptors. We have examined the modulatory role of 5-HT in regulation of I(K,Ca) by I(C) using a Ca2+ ionophore in conjunction with the whole cell patch-clamp technique in isolated photoreceptors. 2. The 40-50% reduction of I(K,Ca) by 5-HT was voltage independent. Cd2+ blocked I(Ca) and reduced I(K,Ca) by 70-80%. The remaining 20-30% of I(K,Ca) may result from Ca2+ release from intracellular stores, because I(K,Ca) was further reduced to 5-10% as the pipette ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) was raised from 0.5 to 5 mM. The application of the Ca2+ ionophore A23187, which was designed to produce Ca2+ influx independent of the voltage-activated Ca2+ channels, restored I(K,Ca). 3. The application of A23187 reversed the effects of 5-HT and Cd2+ on I(K,Ca) for experiments lasting 15-20 min. However, for longer time periods (>25 min), complete restoration of I(K,Ca) by A23187 was obtained in the presence of Cd2+ but not 5-HT. These results suggest that for 15 to 20 min exposures the reduction of I(K,Ca) by 5-HT is a consequence of modulation of I(Ca) by 5-HT and not a direct effect of 5-HT on I(K,Ca).

AB - 1. The Ca2+-dependent K+ current (I(K,Ca)) contributes to both the plateau phase of light-elicited generator potentials and enhanced excitability of identified type B photoreceptors of Hermissenda detected after classical conditioning. Serotonergic modulation of membrane conductances mimics some of the effects of conditioning. Serotonin (5-HT) reduces the magnitude of I(K,Ca) and decreases the sustained voltage- activated Ca2+ current (I(Ca)) in type B photoreceptors. We have examined the modulatory role of 5-HT in regulation of I(K,Ca) by I(C) using a Ca2+ ionophore in conjunction with the whole cell patch-clamp technique in isolated photoreceptors. 2. The 40-50% reduction of I(K,Ca) by 5-HT was voltage independent. Cd2+ blocked I(Ca) and reduced I(K,Ca) by 70-80%. The remaining 20-30% of I(K,Ca) may result from Ca2+ release from intracellular stores, because I(K,Ca) was further reduced to 5-10% as the pipette ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) was raised from 0.5 to 5 mM. The application of the Ca2+ ionophore A23187, which was designed to produce Ca2+ influx independent of the voltage-activated Ca2+ channels, restored I(K,Ca). 3. The application of A23187 reversed the effects of 5-HT and Cd2+ on I(K,Ca) for experiments lasting 15-20 min. However, for longer time periods (>25 min), complete restoration of I(K,Ca) by A23187 was obtained in the presence of Cd2+ but not 5-HT. These results suggest that for 15 to 20 min exposures the reduction of I(K,Ca) by 5-HT is a consequence of modulation of I(Ca) by 5-HT and not a direct effect of 5-HT on I(K,Ca).

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