Abstract
To reduce rehabilitation time and improve survival, a practical, objective test is needed to determine if marine birds are contaminated with oil before they enter captivity. The RaPID Assay® and EnviroGardTM immunoassays for detection of polycyclic aromatic hydrocarbons (PAHs) in soil were evaluated for their ability to detect petroleum on feather samples from 30 intermediate fuel oil contaminated and 30 uncontaminated common murres (Uria aalge). Sensitivity, specificity, positive and negative predictive value, precision, and time required to run each assay were determined. The RaPID Assay® was 96.7% sensitive and specific, while the EnviroGardTM assay was 93.3% sensitive, and 90.0% specific. Sensitivity decreased at higher dilutions for both assays. Intra-replicate variation was less than 20%. Our evaluation showed that these immunoassays are rapid and cost-effective methods for detecting oil-contamination on the plumage of seabirds, with the EnviroGardTM assay being more practical in most oil spill response situations due to ease of use and rapidity of results.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 388-395 |
| Number of pages | 8 |
| Journal | Marine Pollution Bulletin |
| Volume | 44 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2002 |
Fingerprint
Keywords
- Common murres
- Immunoassay
- Oil spills
- PAH
- Rehabilitation
- Uria aalge
ASJC Scopus subject areas
- Aquatic Science
- Oceanography
- Pollution
Cite this
Evaluation of two direct immunoassays for rapid detection of petroleum products on marine birds. / Fritcher, Deana L.; Mazet, Jonna A; Ziccardi, Michael H; Gardner, Ian.
In: Marine Pollution Bulletin, Vol. 44, No. 5, 2002, p. 388-395.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of two direct immunoassays for rapid detection of petroleum products on marine birds
AU - Fritcher, Deana L.
AU - Mazet, Jonna A
AU - Ziccardi, Michael H
AU - Gardner, Ian
PY - 2002
Y1 - 2002
N2 - To reduce rehabilitation time and improve survival, a practical, objective test is needed to determine if marine birds are contaminated with oil before they enter captivity. The RaPID Assay® and EnviroGardTM immunoassays for detection of polycyclic aromatic hydrocarbons (PAHs) in soil were evaluated for their ability to detect petroleum on feather samples from 30 intermediate fuel oil contaminated and 30 uncontaminated common murres (Uria aalge). Sensitivity, specificity, positive and negative predictive value, precision, and time required to run each assay were determined. The RaPID Assay® was 96.7% sensitive and specific, while the EnviroGardTM assay was 93.3% sensitive, and 90.0% specific. Sensitivity decreased at higher dilutions for both assays. Intra-replicate variation was less than 20%. Our evaluation showed that these immunoassays are rapid and cost-effective methods for detecting oil-contamination on the plumage of seabirds, with the EnviroGardTM assay being more practical in most oil spill response situations due to ease of use and rapidity of results.
AB - To reduce rehabilitation time and improve survival, a practical, objective test is needed to determine if marine birds are contaminated with oil before they enter captivity. The RaPID Assay® and EnviroGardTM immunoassays for detection of polycyclic aromatic hydrocarbons (PAHs) in soil were evaluated for their ability to detect petroleum on feather samples from 30 intermediate fuel oil contaminated and 30 uncontaminated common murres (Uria aalge). Sensitivity, specificity, positive and negative predictive value, precision, and time required to run each assay were determined. The RaPID Assay® was 96.7% sensitive and specific, while the EnviroGardTM assay was 93.3% sensitive, and 90.0% specific. Sensitivity decreased at higher dilutions for both assays. Intra-replicate variation was less than 20%. Our evaluation showed that these immunoassays are rapid and cost-effective methods for detecting oil-contamination on the plumage of seabirds, with the EnviroGardTM assay being more practical in most oil spill response situations due to ease of use and rapidity of results.
KW - Common murres
KW - Immunoassay
KW - Oil spills
KW - PAH
KW - Rehabilitation
KW - Uria aalge
UR - http://www.scopus.com/inward/record.url?scp=0035992231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035992231&partnerID=8YFLogxK
U2 - 10.1016/S0025-326X(01)00247-8
DO - 10.1016/S0025-326X(01)00247-8
M3 - Article
C2 - 12146821
AN - SCOPUS:0035992231
VL - 44
SP - 388
EP - 395
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
SN - 0025-326X
IS - 5
ER -