Evaluation of the protective effect of Rhei Radix et Rhizoma against α-naphthylisothiocyanate induced liver injury based on metabolic profile of bile acids

Fan Yang, Ying Xu, Aizhen Xiong, Yugi He, Li Yang, Yu-Jui Yvonne Wan, Zhengtao Wang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Ethnopharmacological relevance: To evaluate the hepatoprotective effect of the root and rhizome of Rhubarb (Rhei Radix et Rhizoma) against α-naphthylisothiocyanate (ANIT)-induced liver injury using metabolic profile of bile acids (BAs) along with biochemical parameters and histological alterations. Materials and methods: Ultra-performance liquid chromatography coupled with quadrupole mass spectrometry (UPLC-MS) was applied to determinate the concentration of BAs, which was followed by multivariate statistical analysis of Principal Component Analysis (PCA) and Partial Least Squares Discriminate Analysis (PLS-DA). Results: Based on PCA results, three groups (Vehicle group, ANIT group and RhO+ANIT group) were clearly distinguished. Tauro-cholic acid (TCA), tauro-hyodesoxycholic acid (THDCA), glyco-cholic acid (GCA), and glyco-chenodeoxycholic acid (GCDCA) were proved to be the most important markers corresponding to ANIT-induced liver injury and protection provided by Rhubarb, which is further confirmed by PLS-DA. A correlation was found between the foregoing BAs and biochemical parameters including serum aspartate aminotransferase (ALT) and aspartate aminotransferase (AST), which confirmed that TCA, THDAC, GCA, and GCDCA could be considered as sensitive biomarkers. Conclusion: The variance of the BAs contents can be used to evaluate ANIT-induced hepatotoxicity caused by ANIT and protective effects of Rhubarb. It also lays the foundation for the further research on the mechanisms of cholestasis as well as the therapeutic effect of Rhubarb.

Original languageEnglish (US)
Pages (from-to)599-604
Number of pages6
JournalJournal of Ethnopharmacology
Volume144
Issue number3
DOIs
StatePublished - Dec 18 2012

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Rheum
Cholic Acid
Metabolome
Bile Acids and Salts
Chenodeoxycholic Acid
Liver
Wounds and Injuries
Aspartate Aminotransferases
Principal Component Analysis
Least-Squares Analysis
Rhizome
Cholestasis
Therapeutic Uses
Liquid Chromatography
Mass Spectrometry
Multivariate Analysis
Biomarkers
Serum
Research

Keywords

  • α-naphthylisothiocyanate
  • Bile acids
  • Hepatoprotective effect
  • Multivariate statistical analysis
  • Rhei radix et Rhizoma
  • Rhubarb

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Evaluation of the protective effect of Rhei Radix et Rhizoma against α-naphthylisothiocyanate induced liver injury based on metabolic profile of bile acids. / Yang, Fan; Xu, Ying; Xiong, Aizhen; He, Yugi; Yang, Li; Wan, Yu-Jui Yvonne; Wang, Zhengtao.

In: Journal of Ethnopharmacology, Vol. 144, No. 3, 18.12.2012, p. 599-604.

Research output: Contribution to journalArticle

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abstract = "Ethnopharmacological relevance: To evaluate the hepatoprotective effect of the root and rhizome of Rhubarb (Rhei Radix et Rhizoma) against α-naphthylisothiocyanate (ANIT)-induced liver injury using metabolic profile of bile acids (BAs) along with biochemical parameters and histological alterations. Materials and methods: Ultra-performance liquid chromatography coupled with quadrupole mass spectrometry (UPLC-MS) was applied to determinate the concentration of BAs, which was followed by multivariate statistical analysis of Principal Component Analysis (PCA) and Partial Least Squares Discriminate Analysis (PLS-DA). Results: Based on PCA results, three groups (Vehicle group, ANIT group and RhO+ANIT group) were clearly distinguished. Tauro-cholic acid (TCA), tauro-hyodesoxycholic acid (THDCA), glyco-cholic acid (GCA), and glyco-chenodeoxycholic acid (GCDCA) were proved to be the most important markers corresponding to ANIT-induced liver injury and protection provided by Rhubarb, which is further confirmed by PLS-DA. A correlation was found between the foregoing BAs and biochemical parameters including serum aspartate aminotransferase (ALT) and aspartate aminotransferase (AST), which confirmed that TCA, THDAC, GCA, and GCDCA could be considered as sensitive biomarkers. Conclusion: The variance of the BAs contents can be used to evaluate ANIT-induced hepatotoxicity caused by ANIT and protective effects of Rhubarb. It also lays the foundation for the further research on the mechanisms of cholestasis as well as the therapeutic effect of Rhubarb.",
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AU - Wan, Yu-Jui Yvonne

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