Abstract
Ethnopharmacological relevance: To evaluate the hepatoprotective effect of the root and rhizome of Rhubarb (Rhei Radix et Rhizoma) against α-naphthylisothiocyanate (ANIT)-induced liver injury using metabolic profile of bile acids (BAs) along with biochemical parameters and histological alterations. Materials and methods: Ultra-performance liquid chromatography coupled with quadrupole mass spectrometry (UPLC-MS) was applied to determinate the concentration of BAs, which was followed by multivariate statistical analysis of Principal Component Analysis (PCA) and Partial Least Squares Discriminate Analysis (PLS-DA). Results: Based on PCA results, three groups (Vehicle group, ANIT group and RhO+ANIT group) were clearly distinguished. Tauro-cholic acid (TCA), tauro-hyodesoxycholic acid (THDCA), glyco-cholic acid (GCA), and glyco-chenodeoxycholic acid (GCDCA) were proved to be the most important markers corresponding to ANIT-induced liver injury and protection provided by Rhubarb, which is further confirmed by PLS-DA. A correlation was found between the foregoing BAs and biochemical parameters including serum aspartate aminotransferase (ALT) and aspartate aminotransferase (AST), which confirmed that TCA, THDAC, GCA, and GCDCA could be considered as sensitive biomarkers. Conclusion: The variance of the BAs contents can be used to evaluate ANIT-induced hepatotoxicity caused by ANIT and protective effects of Rhubarb. It also lays the foundation for the further research on the mechanisms of cholestasis as well as the therapeutic effect of Rhubarb.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 599-604 |
| Number of pages | 6 |
| Journal | Journal of Ethnopharmacology |
| Volume | 144 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 18 2012 |
Fingerprint
Keywords
- α-naphthylisothiocyanate
- Bile acids
- Hepatoprotective effect
- Multivariate statistical analysis
- Rhei radix et Rhizoma
- Rhubarb
ASJC Scopus subject areas
- Pharmacology
- Drug Discovery
Cite this
Evaluation of the protective effect of Rhei Radix et Rhizoma against α-naphthylisothiocyanate induced liver injury based on metabolic profile of bile acids. / Yang, Fan; Xu, Ying; Xiong, Aizhen; He, Yugi; Yang, Li; Wan, Yu-Jui Yvonne; Wang, Zhengtao.
In: Journal of Ethnopharmacology, Vol. 144, No. 3, 18.12.2012, p. 599-604.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the protective effect of Rhei Radix et Rhizoma against α-naphthylisothiocyanate induced liver injury based on metabolic profile of bile acids
AU - Yang, Fan
AU - Xu, Ying
AU - Xiong, Aizhen
AU - He, Yugi
AU - Yang, Li
AU - Wan, Yu-Jui Yvonne
AU - Wang, Zhengtao
PY - 2012/12/18
Y1 - 2012/12/18
N2 - Ethnopharmacological relevance: To evaluate the hepatoprotective effect of the root and rhizome of Rhubarb (Rhei Radix et Rhizoma) against α-naphthylisothiocyanate (ANIT)-induced liver injury using metabolic profile of bile acids (BAs) along with biochemical parameters and histological alterations. Materials and methods: Ultra-performance liquid chromatography coupled with quadrupole mass spectrometry (UPLC-MS) was applied to determinate the concentration of BAs, which was followed by multivariate statistical analysis of Principal Component Analysis (PCA) and Partial Least Squares Discriminate Analysis (PLS-DA). Results: Based on PCA results, three groups (Vehicle group, ANIT group and RhO+ANIT group) were clearly distinguished. Tauro-cholic acid (TCA), tauro-hyodesoxycholic acid (THDCA), glyco-cholic acid (GCA), and glyco-chenodeoxycholic acid (GCDCA) were proved to be the most important markers corresponding to ANIT-induced liver injury and protection provided by Rhubarb, which is further confirmed by PLS-DA. A correlation was found between the foregoing BAs and biochemical parameters including serum aspartate aminotransferase (ALT) and aspartate aminotransferase (AST), which confirmed that TCA, THDAC, GCA, and GCDCA could be considered as sensitive biomarkers. Conclusion: The variance of the BAs contents can be used to evaluate ANIT-induced hepatotoxicity caused by ANIT and protective effects of Rhubarb. It also lays the foundation for the further research on the mechanisms of cholestasis as well as the therapeutic effect of Rhubarb.
AB - Ethnopharmacological relevance: To evaluate the hepatoprotective effect of the root and rhizome of Rhubarb (Rhei Radix et Rhizoma) against α-naphthylisothiocyanate (ANIT)-induced liver injury using metabolic profile of bile acids (BAs) along with biochemical parameters and histological alterations. Materials and methods: Ultra-performance liquid chromatography coupled with quadrupole mass spectrometry (UPLC-MS) was applied to determinate the concentration of BAs, which was followed by multivariate statistical analysis of Principal Component Analysis (PCA) and Partial Least Squares Discriminate Analysis (PLS-DA). Results: Based on PCA results, three groups (Vehicle group, ANIT group and RhO+ANIT group) were clearly distinguished. Tauro-cholic acid (TCA), tauro-hyodesoxycholic acid (THDCA), glyco-cholic acid (GCA), and glyco-chenodeoxycholic acid (GCDCA) were proved to be the most important markers corresponding to ANIT-induced liver injury and protection provided by Rhubarb, which is further confirmed by PLS-DA. A correlation was found between the foregoing BAs and biochemical parameters including serum aspartate aminotransferase (ALT) and aspartate aminotransferase (AST), which confirmed that TCA, THDAC, GCA, and GCDCA could be considered as sensitive biomarkers. Conclusion: The variance of the BAs contents can be used to evaluate ANIT-induced hepatotoxicity caused by ANIT and protective effects of Rhubarb. It also lays the foundation for the further research on the mechanisms of cholestasis as well as the therapeutic effect of Rhubarb.
KW - α-naphthylisothiocyanate
KW - Bile acids
KW - Hepatoprotective effect
KW - Multivariate statistical analysis
KW - Rhei radix et Rhizoma
KW - Rhubarb
UR - http://www.scopus.com/inward/record.url?scp=84870302967&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870302967&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2012.09.049
DO - 10.1016/j.jep.2012.09.049
M3 - Article
C2 - 23058990
AN - SCOPUS:84870302967
VL - 144
SP - 599
EP - 604
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
SN - 0378-8741
IS - 3
ER -