Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis

R. P. Kowalski, M. Uhrin, L. M. Karenchak, Richard L Sweet, Y. J. Gordon

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Purpose: A new polymerase chain reaction (PCR) test (Amplicor, Diagnostics, Branchburg, NJ) was evaluated for its ability to detect chlamydial DNA from previously obtained adult conjunctival specimens. Methods: The sensitivity of this PCR test was determined on 42 adult conjunctival specimens that were culture-positive for Chlamydia. The specificity was determined by testing 40 true-negative specimens that included 10 normal conjunctival samples and 20 ocular specimens that were culture-positive for herpes simplex virus or adenovirus. The remaining ten samples consisted of ocular bacterial pathogens in chlamydial transport media. Results: Amplicor was 88% (37/42) sensitive and 100% (40/40) specific. Conclusions: The authors conclude that PCR testing for chlamydial DNA from ocular specimens may be useful, especially when conditions in transport might reduce the yield of positive cultures.

Original languageEnglish (US)
Pages (from-to)1016-1019
Number of pages4
JournalOphthalmology
Volume102
Issue number7
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

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    Kowalski, R. P., Uhrin, M., Karenchak, L. M., Sweet, R. L., & Gordon, Y. J. (1995). Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis. Ophthalmology, 102(7), 1016-1019.