Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis

R. P. Kowalski, M. Uhrin, L. M. Karenchak, Richard L Sweet, Y. J. Gordon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Purpose: A new polymerase chain reaction (PCR) test (Amplicor, Diagnostics, Branchburg, NJ) was evaluated for its ability to detect chlamydial DNA from previously obtained adult conjunctival specimens. Methods: The sensitivity of this PCR test was determined on 42 adult conjunctival specimens that were culture-positive for Chlamydia. The specificity was determined by testing 40 true-negative specimens that included 10 normal conjunctival samples and 20 ocular specimens that were culture-positive for herpes simplex virus or adenovirus. The remaining ten samples consisted of ocular bacterial pathogens in chlamydial transport media. Results: Amplicor was 88% (37/42) sensitive and 100% (40/40) specific. Conclusions: The authors conclude that PCR testing for chlamydial DNA from ocular specimens may be useful, especially when conditions in transport might reduce the yield of positive cultures.

Original languageEnglish (US)
Pages (from-to)1016-1019
Number of pages4
JournalOphthalmology
Volume102
Issue number7
StatePublished - 1995
Externally publishedYes

Fingerprint

Conjunctivitis
Polymerase Chain Reaction
DNA
Chlamydia
Simplexvirus
Routine Diagnostic Tests
Adenoviridae

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Kowalski, R. P., Uhrin, M., Karenchak, L. M., Sweet, R. L., & Gordon, Y. J. (1995). Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis. Ophthalmology, 102(7), 1016-1019.

Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis. / Kowalski, R. P.; Uhrin, M.; Karenchak, L. M.; Sweet, Richard L; Gordon, Y. J.

In: Ophthalmology, Vol. 102, No. 7, 1995, p. 1016-1019.

Research output: Contribution to journalArticle

Kowalski, RP, Uhrin, M, Karenchak, LM, Sweet, RL & Gordon, YJ 1995, 'Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis', Ophthalmology, vol. 102, no. 7, pp. 1016-1019.
Kowalski, R. P. ; Uhrin, M. ; Karenchak, L. M. ; Sweet, Richard L ; Gordon, Y. J. / Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis. In: Ophthalmology. 1995 ; Vol. 102, No. 7. pp. 1016-1019.
@article{05fc0a85ddb64c35a2563ff8b44cf1f7,
title = "Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis",
abstract = "Purpose: A new polymerase chain reaction (PCR) test (Amplicor, Diagnostics, Branchburg, NJ) was evaluated for its ability to detect chlamydial DNA from previously obtained adult conjunctival specimens. Methods: The sensitivity of this PCR test was determined on 42 adult conjunctival specimens that were culture-positive for Chlamydia. The specificity was determined by testing 40 true-negative specimens that included 10 normal conjunctival samples and 20 ocular specimens that were culture-positive for herpes simplex virus or adenovirus. The remaining ten samples consisted of ocular bacterial pathogens in chlamydial transport media. Results: Amplicor was 88{\%} (37/42) sensitive and 100{\%} (40/40) specific. Conclusions: The authors conclude that PCR testing for chlamydial DNA from ocular specimens may be useful, especially when conditions in transport might reduce the yield of positive cultures.",
author = "Kowalski, {R. P.} and M. Uhrin and Karenchak, {L. M.} and Sweet, {Richard L} and Gordon, {Y. J.}",
year = "1995",
language = "English (US)",
volume = "102",
pages = "1016--1019",
journal = "Ophthalmology",
issn = "0161-6420",
publisher = "Elsevier Inc.",
number = "7",

}

TY - JOUR

T1 - Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis

AU - Kowalski, R. P.

AU - Uhrin, M.

AU - Karenchak, L. M.

AU - Sweet, Richard L

AU - Gordon, Y. J.

PY - 1995

Y1 - 1995

N2 - Purpose: A new polymerase chain reaction (PCR) test (Amplicor, Diagnostics, Branchburg, NJ) was evaluated for its ability to detect chlamydial DNA from previously obtained adult conjunctival specimens. Methods: The sensitivity of this PCR test was determined on 42 adult conjunctival specimens that were culture-positive for Chlamydia. The specificity was determined by testing 40 true-negative specimens that included 10 normal conjunctival samples and 20 ocular specimens that were culture-positive for herpes simplex virus or adenovirus. The remaining ten samples consisted of ocular bacterial pathogens in chlamydial transport media. Results: Amplicor was 88% (37/42) sensitive and 100% (40/40) specific. Conclusions: The authors conclude that PCR testing for chlamydial DNA from ocular specimens may be useful, especially when conditions in transport might reduce the yield of positive cultures.

AB - Purpose: A new polymerase chain reaction (PCR) test (Amplicor, Diagnostics, Branchburg, NJ) was evaluated for its ability to detect chlamydial DNA from previously obtained adult conjunctival specimens. Methods: The sensitivity of this PCR test was determined on 42 adult conjunctival specimens that were culture-positive for Chlamydia. The specificity was determined by testing 40 true-negative specimens that included 10 normal conjunctival samples and 20 ocular specimens that were culture-positive for herpes simplex virus or adenovirus. The remaining ten samples consisted of ocular bacterial pathogens in chlamydial transport media. Results: Amplicor was 88% (37/42) sensitive and 100% (40/40) specific. Conclusions: The authors conclude that PCR testing for chlamydial DNA from ocular specimens may be useful, especially when conditions in transport might reduce the yield of positive cultures.

UR - http://www.scopus.com/inward/record.url?scp=0029166001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029166001&partnerID=8YFLogxK

M3 - Article

VL - 102

SP - 1016

EP - 1019

JO - Ophthalmology

JF - Ophthalmology

SN - 0161-6420

IS - 7

ER -