Evaluation of the pathogenic potential of cervid adenovirus in calves

Leslie Woods, Howard D. Lehmkuhl, Lea Ann Hobbs, Jackie C. Parker, Mike Manzer

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Four 3-month-old Jersey calves and three 3-month-old Holstein calves were inoculated with cervid adenovirus and monitored for clinical signs until necropsied between 10 and 42 days postinoculation. The neonatal Jersey calves had received colostrum, and the Holstein calves were colostrum deprived. Preinoculation and postinoculation serum samples were tested for antibodies to the cervid adenovirus, bovine adenovirus type 6, bovine adenovirus type 7, and goat adenovirus type 1. Virus isolation was performed on kidney, nasal secretion, and/or lung homogenates in fetal white-tailed deer lung cells. Negatively stained preparations of feces from Jersey calves were examined weekly using an electron microscope, and weekly blood samples were collected for complete blood counts. Full necropsies were performed on all calves. A complete selection of tissues was evaluated for microscopic changes, and immunohistochemistry was performed on all tissues using a polyclonal antibody to deer adenovirus. No clinical signs were observed in the calves during the study period. Following inoculation, colostrum-deprived calves developed low antibody titers to deer adenovirus, while the Jersey calves that received colostrum did not. Calves that received colostrum had high antibody titers to bovine adenovirus type 7 and goat adenovirus type 1. No consistent gross or microscopic lesions were seen. Adenovirus was not observed in negatively stained preparations of feces. Immunohistochemistry results did not demonstrate virus in all tissues examined microscopically, and virus was not isolated from lungs, nasal secretions, and kidneys.

Original languageEnglish (US)
Pages (from-to)33-37
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Issue number1
StatePublished - Jan 1 2008


  • Adenovirus
  • Bovine
  • Cervid adenovirus
  • Experimental
  • Odocoileus adenovirus

ASJC Scopus subject areas

  • veterinary(all)


Dive into the research topics of 'Evaluation of the pathogenic potential of cervid adenovirus in calves'. Together they form a unique fingerprint.

Cite this