Evaluation of the microcirculation of the equine small intestine after intraluminal distention and subsequent decompression.

R. M. Dabareiner, K. E. Sullins, J. R. Snyder, N. A. White, Ian Gardner

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Effects of intraluminal distention (25 cm of H2O, 120 minutes) and subsequent decompression (60 minutes) on intramural vascular patterns of the small intestine was evaluated in 7 anesthetized horses. Intraluminal distention (25 cm of H2O, 120 minutes) was created in 2 jejunal segments in each horse. Experimental and control segments were removed either immediately after the experimental period or after 60 minutes of decompression. The vascular system of experimental and control jejunal segments was lavaged with NaCl, then was injected with a blue-colored radiopaque medium for microangiography or with a diluted methyl methacrylate for scanning electron microscopy of microcorrosion vascular casts. After angiographic evaluation, tissue sections were prepared for light microscopic evaluation to assess vascular filling and tissue morphology. The distended segments had short villi, which were separated by expanded crypts, and had mesothelial cell loss, neutrophil infiltration, and edema in the seromuscular layer. The number of perfused vessels was significantly (P < 0.05) decreased in the seromuscular layer and, to a lesser extent, in the mucosal layer of the distended segments, compared with controls. After decompression, the morphologic lesions progressed in mucosal and serosal layers and the number of observed vessels increased in all intramural layers; however, vascular density did not return to the predistention state. These results identify altered intramural vascular patterns in the equine jejunum during luminal distention and subsequent decompression.

Original languageEnglish (US)
Pages (from-to)1673-1682
Number of pages10
JournalAmerican Journal of Veterinary Research
Volume54
Issue number10
StatePublished - Oct 1 1993
Externally publishedYes

Fingerprint

Microcirculation
Decompression
blood vessels
Horses
Small Intestine
Blood Vessels
small intestine
horses
villi
jejunum
Methacrylates
Neutrophil Infiltration
lesions (animal)
edema
Jejunum
neutrophils
scanning electron microscopy
Electron Scanning Microscopy
Contrast Media
Edema

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of the microcirculation of the equine small intestine after intraluminal distention and subsequent decompression. / Dabareiner, R. M.; Sullins, K. E.; Snyder, J. R.; White, N. A.; Gardner, Ian.

In: American Journal of Veterinary Research, Vol. 54, No. 10, 01.10.1993, p. 1673-1682.

Research output: Contribution to journalArticle

@article{ad8a9c199747461cb05fe108632331b6,
title = "Evaluation of the microcirculation of the equine small intestine after intraluminal distention and subsequent decompression.",
abstract = "Effects of intraluminal distention (25 cm of H2O, 120 minutes) and subsequent decompression (60 minutes) on intramural vascular patterns of the small intestine was evaluated in 7 anesthetized horses. Intraluminal distention (25 cm of H2O, 120 minutes) was created in 2 jejunal segments in each horse. Experimental and control segments were removed either immediately after the experimental period or after 60 minutes of decompression. The vascular system of experimental and control jejunal segments was lavaged with NaCl, then was injected with a blue-colored radiopaque medium for microangiography or with a diluted methyl methacrylate for scanning electron microscopy of microcorrosion vascular casts. After angiographic evaluation, tissue sections were prepared for light microscopic evaluation to assess vascular filling and tissue morphology. The distended segments had short villi, which were separated by expanded crypts, and had mesothelial cell loss, neutrophil infiltration, and edema in the seromuscular layer. The number of perfused vessels was significantly (P < 0.05) decreased in the seromuscular layer and, to a lesser extent, in the mucosal layer of the distended segments, compared with controls. After decompression, the morphologic lesions progressed in mucosal and serosal layers and the number of observed vessels increased in all intramural layers; however, vascular density did not return to the predistention state. These results identify altered intramural vascular patterns in the equine jejunum during luminal distention and subsequent decompression.",
author = "Dabareiner, {R. M.} and Sullins, {K. E.} and Snyder, {J. R.} and White, {N. A.} and Ian Gardner",
year = "1993",
month = "10",
day = "1",
language = "English (US)",
volume = "54",
pages = "1673--1682",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "10",

}

TY - JOUR

T1 - Evaluation of the microcirculation of the equine small intestine after intraluminal distention and subsequent decompression.

AU - Dabareiner, R. M.

AU - Sullins, K. E.

AU - Snyder, J. R.

AU - White, N. A.

AU - Gardner, Ian

PY - 1993/10/1

Y1 - 1993/10/1

N2 - Effects of intraluminal distention (25 cm of H2O, 120 minutes) and subsequent decompression (60 minutes) on intramural vascular patterns of the small intestine was evaluated in 7 anesthetized horses. Intraluminal distention (25 cm of H2O, 120 minutes) was created in 2 jejunal segments in each horse. Experimental and control segments were removed either immediately after the experimental period or after 60 minutes of decompression. The vascular system of experimental and control jejunal segments was lavaged with NaCl, then was injected with a blue-colored radiopaque medium for microangiography or with a diluted methyl methacrylate for scanning electron microscopy of microcorrosion vascular casts. After angiographic evaluation, tissue sections were prepared for light microscopic evaluation to assess vascular filling and tissue morphology. The distended segments had short villi, which were separated by expanded crypts, and had mesothelial cell loss, neutrophil infiltration, and edema in the seromuscular layer. The number of perfused vessels was significantly (P < 0.05) decreased in the seromuscular layer and, to a lesser extent, in the mucosal layer of the distended segments, compared with controls. After decompression, the morphologic lesions progressed in mucosal and serosal layers and the number of observed vessels increased in all intramural layers; however, vascular density did not return to the predistention state. These results identify altered intramural vascular patterns in the equine jejunum during luminal distention and subsequent decompression.

AB - Effects of intraluminal distention (25 cm of H2O, 120 minutes) and subsequent decompression (60 minutes) on intramural vascular patterns of the small intestine was evaluated in 7 anesthetized horses. Intraluminal distention (25 cm of H2O, 120 minutes) was created in 2 jejunal segments in each horse. Experimental and control segments were removed either immediately after the experimental period or after 60 minutes of decompression. The vascular system of experimental and control jejunal segments was lavaged with NaCl, then was injected with a blue-colored radiopaque medium for microangiography or with a diluted methyl methacrylate for scanning electron microscopy of microcorrosion vascular casts. After angiographic evaluation, tissue sections were prepared for light microscopic evaluation to assess vascular filling and tissue morphology. The distended segments had short villi, which were separated by expanded crypts, and had mesothelial cell loss, neutrophil infiltration, and edema in the seromuscular layer. The number of perfused vessels was significantly (P < 0.05) decreased in the seromuscular layer and, to a lesser extent, in the mucosal layer of the distended segments, compared with controls. After decompression, the morphologic lesions progressed in mucosal and serosal layers and the number of observed vessels increased in all intramural layers; however, vascular density did not return to the predistention state. These results identify altered intramural vascular patterns in the equine jejunum during luminal distention and subsequent decompression.

UR - http://www.scopus.com/inward/record.url?scp=0027675412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027675412&partnerID=8YFLogxK

M3 - Article

C2 - 8250393

AN - SCOPUS:0027675412

VL - 54

SP - 1673

EP - 1682

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 10

ER -