Abstract
Objective: To evaluate the effect of hemodialysis, hemoperfusion, and a combined approach on the removal of metaldehyde from canine plasma. Design: In vitro study. Setting: University veterinary teaching hospital laboratory. Animals: None. Interventions: None. Measurements and Main Results: Metaldehyde-fortified canine plasma was prepared. Hemodialysis (HD), charcoal hemoperfusion (HP), and in-series hemodialysis and charcoal hemoperfusion (HD/HP) were applied in triplicate to eliminate metaldehyde from plasma. Plasma samples were obtained before starting the procedure and subsequently after every processed total plasma volume until plasma had been processed 10 times. Plasma metaldehyde concentration was quantitatively assayed by gas chromatography-mass spectrometry. Statistical analysis was performed using one-way ANOVA, repeated measures ANOVA, and Bonferroni post hoc test, and by calculating the coefficient of variation from duplicate measurements, binomial distribution, and by Bland–Altman analysis. Statistically significant reduction in metaldehyde concentration was reached by all 3 techniques. Reduction of metaldehyde concentration of more than 95% was achieved after processing the plasma volume 4 times applying HD, 8 times applying HP, and 2 times applying HD/HP. Efficacy in reduction of metaldehyde concentration differed significantly between the 3 procedures (P < 0.001). In-series hemodialysis and charcoal hemoperfusion was more effective in metaldehyde removal than HD (P = 0.003) and HP (P < 0.001), and HD was more effective than HP (P = 0.002). Conclusions: Metaldehyde was effectively removed by all applied extracorporeal blood purification techniques in vitro. However, the combination of both techniques was more effective than HD or HP alone. Further clinical studies are warranted to confirm therapeutic benefits in patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 516-523 |
| Number of pages | 8 |
| Journal | Journal of Veterinary Emergency and Critical Care |
| Volume | 26 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 1 2016 |
Fingerprint
Keywords
- decontamination
- dialysis
- dog
- extracorporeal blood purification
- molluscicide
- snail bait
ASJC Scopus subject areas
- veterinary(all)
Cite this
Evaluation of the in vitro efficacy of hemodialysis, hemoperfusion, and the combined approach on the removal of metaldehyde from canine plasma. / Mauser, Elisabeth L.; Puschner, Birgit; Reese, Sven; Hartmann, Katrin; Doerfelt, René.
In: Journal of Veterinary Emergency and Critical Care, Vol. 26, No. 4, 01.07.2016, p. 516-523.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the in vitro efficacy of hemodialysis, hemoperfusion, and the combined approach on the removal of metaldehyde from canine plasma
AU - Mauser, Elisabeth L.
AU - Puschner, Birgit
AU - Reese, Sven
AU - Hartmann, Katrin
AU - Doerfelt, René
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Objective: To evaluate the effect of hemodialysis, hemoperfusion, and a combined approach on the removal of metaldehyde from canine plasma. Design: In vitro study. Setting: University veterinary teaching hospital laboratory. Animals: None. Interventions: None. Measurements and Main Results: Metaldehyde-fortified canine plasma was prepared. Hemodialysis (HD), charcoal hemoperfusion (HP), and in-series hemodialysis and charcoal hemoperfusion (HD/HP) were applied in triplicate to eliminate metaldehyde from plasma. Plasma samples were obtained before starting the procedure and subsequently after every processed total plasma volume until plasma had been processed 10 times. Plasma metaldehyde concentration was quantitatively assayed by gas chromatography-mass spectrometry. Statistical analysis was performed using one-way ANOVA, repeated measures ANOVA, and Bonferroni post hoc test, and by calculating the coefficient of variation from duplicate measurements, binomial distribution, and by Bland–Altman analysis. Statistically significant reduction in metaldehyde concentration was reached by all 3 techniques. Reduction of metaldehyde concentration of more than 95% was achieved after processing the plasma volume 4 times applying HD, 8 times applying HP, and 2 times applying HD/HP. Efficacy in reduction of metaldehyde concentration differed significantly between the 3 procedures (P < 0.001). In-series hemodialysis and charcoal hemoperfusion was more effective in metaldehyde removal than HD (P = 0.003) and HP (P < 0.001), and HD was more effective than HP (P = 0.002). Conclusions: Metaldehyde was effectively removed by all applied extracorporeal blood purification techniques in vitro. However, the combination of both techniques was more effective than HD or HP alone. Further clinical studies are warranted to confirm therapeutic benefits in patients.
AB - Objective: To evaluate the effect of hemodialysis, hemoperfusion, and a combined approach on the removal of metaldehyde from canine plasma. Design: In vitro study. Setting: University veterinary teaching hospital laboratory. Animals: None. Interventions: None. Measurements and Main Results: Metaldehyde-fortified canine plasma was prepared. Hemodialysis (HD), charcoal hemoperfusion (HP), and in-series hemodialysis and charcoal hemoperfusion (HD/HP) were applied in triplicate to eliminate metaldehyde from plasma. Plasma samples were obtained before starting the procedure and subsequently after every processed total plasma volume until plasma had been processed 10 times. Plasma metaldehyde concentration was quantitatively assayed by gas chromatography-mass spectrometry. Statistical analysis was performed using one-way ANOVA, repeated measures ANOVA, and Bonferroni post hoc test, and by calculating the coefficient of variation from duplicate measurements, binomial distribution, and by Bland–Altman analysis. Statistically significant reduction in metaldehyde concentration was reached by all 3 techniques. Reduction of metaldehyde concentration of more than 95% was achieved after processing the plasma volume 4 times applying HD, 8 times applying HP, and 2 times applying HD/HP. Efficacy in reduction of metaldehyde concentration differed significantly between the 3 procedures (P < 0.001). In-series hemodialysis and charcoal hemoperfusion was more effective in metaldehyde removal than HD (P = 0.003) and HP (P < 0.001), and HD was more effective than HP (P = 0.002). Conclusions: Metaldehyde was effectively removed by all applied extracorporeal blood purification techniques in vitro. However, the combination of both techniques was more effective than HD or HP alone. Further clinical studies are warranted to confirm therapeutic benefits in patients.
KW - decontamination
KW - dialysis
KW - dog
KW - extracorporeal blood purification
KW - molluscicide
KW - snail bait
UR - http://www.scopus.com/inward/record.url?scp=84978993698&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978993698&partnerID=8YFLogxK
U2 - 10.1111/vec.12487
DO - 10.1111/vec.12487
M3 - Article
C2 - 27219514
AN - SCOPUS:84971283899
VL - 26
SP - 516
EP - 523
JO - Journal of Veterinary Emergency and Critical Care
JF - Journal of Veterinary Emergency and Critical Care
SN - 1479-3261
IS - 4
ER -