Abstract
Performance of the Delvo-X-Press β-lactam antibiotic assay was examined using bulk-tank milk samples and milk samples from individual cows. Bulk-tank milk samples fortified with bovine lactoferrin at a concentration of 1 mg/ml or more consistently tested positive. False-positive results were also obtained from bulk-tank milk samples fortified with bovine plasma at concentrations of 20 and 40%. The assay yielded positive results for milk with antibiotic concentrations as low as 2 ppb. Individual milk samples were collected from 144 healthy lactating cows and from 34 cows with chronic Staphylococcus aureus mastiffs. Specificity estimates for samples from healthy and mastitic cows were 0.88 (95% confidence interval [CI], 0.82, 0.93) and 0.94 (95% CI, 0.86, 1.00), respectively. Individual milk samples were collected from three cows with experimentally induced mastitis for 21 consecutive days. False-positive results occurred as late as 12 days postchallenge. A moderate but significant (P < 0.01) positive linear correlation (r = 0.61) was observed between test result and somatic cell count (SCC) values in milk samples with SCCs of >106/ml.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1183-1190 |
| Number of pages | 8 |
| Journal | Journal of Food Protection |
| Volume | 62 |
| Issue number | 10 |
| State | Published - Oct 1999 |
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ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology
- Biotechnology
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Evaluation of the Delvo-X-Press assay for detecting antibiotic residues in milk samples from individual cows. / Angelidis, Apostolos S.; Farver, Thomas B; Cullor, James S.
In: Journal of Food Protection, Vol. 62, No. 10, 10.1999, p. 1183-1190.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the Delvo-X-Press assay for detecting antibiotic residues in milk samples from individual cows
AU - Angelidis, Apostolos S.
AU - Farver, Thomas B
AU - Cullor, James S
PY - 1999/10
Y1 - 1999/10
N2 - Performance of the Delvo-X-Press β-lactam antibiotic assay was examined using bulk-tank milk samples and milk samples from individual cows. Bulk-tank milk samples fortified with bovine lactoferrin at a concentration of 1 mg/ml or more consistently tested positive. False-positive results were also obtained from bulk-tank milk samples fortified with bovine plasma at concentrations of 20 and 40%. The assay yielded positive results for milk with antibiotic concentrations as low as 2 ppb. Individual milk samples were collected from 144 healthy lactating cows and from 34 cows with chronic Staphylococcus aureus mastiffs. Specificity estimates for samples from healthy and mastitic cows were 0.88 (95% confidence interval [CI], 0.82, 0.93) and 0.94 (95% CI, 0.86, 1.00), respectively. Individual milk samples were collected from three cows with experimentally induced mastitis for 21 consecutive days. False-positive results occurred as late as 12 days postchallenge. A moderate but significant (P < 0.01) positive linear correlation (r = 0.61) was observed between test result and somatic cell count (SCC) values in milk samples with SCCs of >106/ml.
AB - Performance of the Delvo-X-Press β-lactam antibiotic assay was examined using bulk-tank milk samples and milk samples from individual cows. Bulk-tank milk samples fortified with bovine lactoferrin at a concentration of 1 mg/ml or more consistently tested positive. False-positive results were also obtained from bulk-tank milk samples fortified with bovine plasma at concentrations of 20 and 40%. The assay yielded positive results for milk with antibiotic concentrations as low as 2 ppb. Individual milk samples were collected from 144 healthy lactating cows and from 34 cows with chronic Staphylococcus aureus mastiffs. Specificity estimates for samples from healthy and mastitic cows were 0.88 (95% confidence interval [CI], 0.82, 0.93) and 0.94 (95% CI, 0.86, 1.00), respectively. Individual milk samples were collected from three cows with experimentally induced mastitis for 21 consecutive days. False-positive results occurred as late as 12 days postchallenge. A moderate but significant (P < 0.01) positive linear correlation (r = 0.61) was observed between test result and somatic cell count (SCC) values in milk samples with SCCs of >106/ml.
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M3 - Article
C2 - 10528724
AN - SCOPUS:0033214649
VL - 62
SP - 1183
EP - 1190
JO - Journal of Food Protection
JF - Journal of Food Protection
SN - 0362-028X
IS - 10
ER -