Evaluation of the contribution of the renal capsule and cortex to kidney autofluorescence intensity under ultraviolet excitation

Rajesh N. Raman, Christopher D. Pivetti, Alexander M. Rubenchik, Dennis L Matthews, Christoph Troppmann, Stavros G. Demos

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to rat kidney autofluorescence of the capsule versus cortex under ultraviolet excitation is determined from experimental results obtained using autofluorescence microscopy and a suitable mathematical model. The results allow for a quantitative assessment of the relative contribution of the signal originating in the metabolically active cortex as a function of capsule thickness for different wavelengths.

Original languageEnglish (US)
Article number020505
JournalJournal of Biomedical Optics
Volume14
Issue number2
DOIs
StatePublished - 2009

Fingerprint

cortexes
kidneys
capsules
NAD
Capsules
Rats
Microscopic examination
Fluorescence
Availability
Mathematical models
nicotinamide
Wavelength
Oxygen
evaluation
adenines
rats
excitation
availability
mathematical models
microscopy

Keywords

  • fluorescence
  • lasers
  • microscopy
  • tissues
  • ultraviolet

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Evaluation of the contribution of the renal capsule and cortex to kidney autofluorescence intensity under ultraviolet excitation. / Raman, Rajesh N.; Pivetti, Christopher D.; Rubenchik, Alexander M.; Matthews, Dennis L; Troppmann, Christoph; Demos, Stavros G.

In: Journal of Biomedical Optics, Vol. 14, No. 2, 020505, 2009.

Research output: Contribution to journalArticle

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AU - Demos, Stavros G.

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