Evaluation of the comparative accuracy of the complement fixation test, Western blot and five enzyme-linked immunosorbent assays for serodiagnosis of glanders

Mandy Carolina Elschner, Karine Laroucau, Harisankar Singha, Bhupendra Nath Tripathi, Muhammad Saqib, Ian Gardner, Sheetal Saini, Subodh Kumar, Hosny El-Adawy, Falk Melzer, Iahtasham Khan, Praveen Malik, Carola Sauter- Louis, Heinrich Neubauer

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Glanders is a zoonotic contagious disease of equids caused by Burkholderia (B.) mallei. Serodiagnosis of the disease is challenging because of false-positive and false-negative test results. The accuracy of the complement fixation test (CFT) which is prescribed for international trade by the World Organisation for Animal Health (OIE), five ELISAs and a Western blot (WB) were compared for serodiagnosis of glanders using sera from 3,000 glandersfree and 254 glanderous equids. Four ELISA tests are based on recombinant antigens (TssA, TssB, BimA and Hcp1), the IDVet ELISA is based on a semi-purified fraction of B. mallei and WB makes use of a purified LPS-containing B. mallei-antigen. Sensitivity and specificity of tests were estimated using cut-off values recommended by the test developers. The WB and all ELISAs, except BimA, were significantly more specific than the CFT. ELISAs based on TssA, TssB, and BimA antigens had significantly lower sensitivity compared to CFT while the sensitivities of the Hcp1-ELISA, the IDVet-ELISA and the WB did not differ significantly from that of the CFT. Given their comparable sensitivities and specificities, the CFT (98.0%, 96.4%), the WB (96.8%, 99.4%), the Hcp1-ELISA (95.3%, 99.6%) and the IDVet-ELISA (92.5%, 99.5%) should be further developed to meet OIE requirements.

Original languageEnglish (US)
Article numbere0214963
JournalPloS one
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2019
Externally publishedYes

Fingerprint

Glanders
glanders
Complement Fixation Tests
Immunosorbents
complement fixation tests
serodiagnosis
Serologic Tests
Assays
Western blotting
Western Blotting
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Antigens
Enzymes
International trade
Malleus
Animals
Health
Burkholderia mallei
testing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Evaluation of the comparative accuracy of the complement fixation test, Western blot and five enzyme-linked immunosorbent assays for serodiagnosis of glanders. / Elschner, Mandy Carolina; Laroucau, Karine; Singha, Harisankar; Tripathi, Bhupendra Nath; Saqib, Muhammad; Gardner, Ian; Saini, Sheetal; Kumar, Subodh; El-Adawy, Hosny; Melzer, Falk; Khan, Iahtasham; Malik, Praveen; Sauter- Louis, Carola; Neubauer, Heinrich.

In: PloS one, Vol. 14, No. 4, e0214963, 01.04.2019.

Research output: Contribution to journalArticle

Elschner, MC, Laroucau, K, Singha, H, Tripathi, BN, Saqib, M, Gardner, I, Saini, S, Kumar, S, El-Adawy, H, Melzer, F, Khan, I, Malik, P, Sauter- Louis, C & Neubauer, H 2019, 'Evaluation of the comparative accuracy of the complement fixation test, Western blot and five enzyme-linked immunosorbent assays for serodiagnosis of glanders', PloS one, vol. 14, no. 4, e0214963. https://doi.org/10.1371/journal.pone.0214963
Elschner, Mandy Carolina ; Laroucau, Karine ; Singha, Harisankar ; Tripathi, Bhupendra Nath ; Saqib, Muhammad ; Gardner, Ian ; Saini, Sheetal ; Kumar, Subodh ; El-Adawy, Hosny ; Melzer, Falk ; Khan, Iahtasham ; Malik, Praveen ; Sauter- Louis, Carola ; Neubauer, Heinrich. / Evaluation of the comparative accuracy of the complement fixation test, Western blot and five enzyme-linked immunosorbent assays for serodiagnosis of glanders. In: PloS one. 2019 ; Vol. 14, No. 4.
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AU - Tripathi, Bhupendra Nath

AU - Saqib, Muhammad

AU - Gardner, Ian

AU - Saini, Sheetal

AU - Kumar, Subodh

AU - El-Adawy, Hosny

AU - Melzer, Falk

AU - Khan, Iahtasham

AU - Malik, Praveen

AU - Sauter- Louis, Carola

AU - Neubauer, Heinrich

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