Evaluation of the colilert® test for detection of verotoxin-producing escherichia coli in water: A tool for on-farm food safety

Scott W. Brooks, James S Cullor, Ian Gardner

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The objective of this study was to estimate the sensitivity of the Colilert® test for detection of different isolates of Escherichia coli (E. coli) O157:H7 and other verotoxin-producing E. coli (VTEC) serotypes from water for potential on-farm food safety application. E. coli isolates evaluated were of human, bovine, porcine, or food origin. The sensitivity of the Colilert® test was 92% overall for the verotoxin-producing serotypes of E. coli. However, none of the E. coli O157:H7 isolates were identified as E. coli. No false-positive results for E. coli were detected. The Colilert® test could be used to screen water supplies for E. coli on the farm, however, an important pathogenic E. coli sero-type is not detected by the Colilert® test.

Original languageEnglish (US)
Pages (from-to)31-41
Number of pages11
JournalJournal of Agromedicine
Volume5
Issue number3
DOIs
StatePublished - Dec 24 1998

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Shiga-Toxigenic Escherichia coli
Food Safety
Escherichia coli
Water
Escherichia coli O157
Water Supply
Swine
Farms
Food

Keywords

  • Colilert®
  • Colorimetric test
  • Escherichia coli
  • Food safety
  • Livestocks water
  • On-farm
  • Verotoxin

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

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title = "Evaluation of the colilert{\circledR} test for detection of verotoxin-producing escherichia coli in water: A tool for on-farm food safety",
abstract = "The objective of this study was to estimate the sensitivity of the Colilert{\circledR} test for detection of different isolates of Escherichia coli (E. coli) O157:H7 and other verotoxin-producing E. coli (VTEC) serotypes from water for potential on-farm food safety application. E. coli isolates evaluated were of human, bovine, porcine, or food origin. The sensitivity of the Colilert{\circledR} test was 92{\%} overall for the verotoxin-producing serotypes of E. coli. However, none of the E. coli O157:H7 isolates were identified as E. coli. No false-positive results for E. coli were detected. The Colilert{\circledR} test could be used to screen water supplies for E. coli on the farm, however, an important pathogenic E. coli sero-type is not detected by the Colilert{\circledR} test.",
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author = "Brooks, {Scott W.} and Cullor, {James S} and Ian Gardner",
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