Abstract
The objective of this study was to estimate the sensitivity of the Colilert® test for detection of different isolates of Escherichia coli (E. coli) O157:H7 and other verotoxin-producing E. coli (VTEC) serotypes from water for potential on-farm food safety application. E. coli isolates evaluated were of human, bovine, porcine, or food origin. The sensitivity of the Colilert® test was 92% overall for the verotoxin-producing serotypes of E. coli. However, none of the E. coli O157:H7 isolates were identified as E. coli. No false-positive results for E. coli were detected. The Colilert® test could be used to screen water supplies for E. coli on the farm, however, an important pathogenic E. coli sero-type is not detected by the Colilert® test.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 31-41 |
| Number of pages | 11 |
| Journal | Journal of Agromedicine |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 24 1998 |
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Keywords
- Colilert®
- Colorimetric test
- Escherichia coli
- Food safety
- Livestocks water
- On-farm
- Verotoxin
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
Cite this
Evaluation of the colilert® test for detection of verotoxin-producing escherichia coli in water : A tool for on-farm food safety. / Brooks, Scott W.; Cullor, James S; Gardner, Ian.
In: Journal of Agromedicine, Vol. 5, No. 3, 24.12.1998, p. 31-41.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the colilert® test for detection of verotoxin-producing escherichia coli in water
T2 - A tool for on-farm food safety
AU - Brooks, Scott W.
AU - Cullor, James S
AU - Gardner, Ian
PY - 1998/12/24
Y1 - 1998/12/24
N2 - The objective of this study was to estimate the sensitivity of the Colilert® test for detection of different isolates of Escherichia coli (E. coli) O157:H7 and other verotoxin-producing E. coli (VTEC) serotypes from water for potential on-farm food safety application. E. coli isolates evaluated were of human, bovine, porcine, or food origin. The sensitivity of the Colilert® test was 92% overall for the verotoxin-producing serotypes of E. coli. However, none of the E. coli O157:H7 isolates were identified as E. coli. No false-positive results for E. coli were detected. The Colilert® test could be used to screen water supplies for E. coli on the farm, however, an important pathogenic E. coli sero-type is not detected by the Colilert® test.
AB - The objective of this study was to estimate the sensitivity of the Colilert® test for detection of different isolates of Escherichia coli (E. coli) O157:H7 and other verotoxin-producing E. coli (VTEC) serotypes from water for potential on-farm food safety application. E. coli isolates evaluated were of human, bovine, porcine, or food origin. The sensitivity of the Colilert® test was 92% overall for the verotoxin-producing serotypes of E. coli. However, none of the E. coli O157:H7 isolates were identified as E. coli. No false-positive results for E. coli were detected. The Colilert® test could be used to screen water supplies for E. coli on the farm, however, an important pathogenic E. coli sero-type is not detected by the Colilert® test.
KW - Colilert®
KW - Colorimetric test
KW - Escherichia coli
KW - Food safety
KW - Livestocks water
KW - On-farm
KW - Verotoxin
UR - http://www.scopus.com/inward/record.url?scp=84888016561&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84888016561&partnerID=8YFLogxK
U2 - 10.1300/J096v05n03_05
DO - 10.1300/J096v05n03_05
M3 - Article
AN - SCOPUS:84888016561
VL - 5
SP - 31
EP - 41
JO - Journal of Agromedicine
JF - Journal of Agromedicine
SN - 1059-924X
IS - 3
ER -