Evaluation of the clinical performance of 2 point-of-care cryptococcal antigen tests in dogs and cats

Research output: Contribution to journalArticle

Abstract

Background: Point-of-care (POC) Cryptococcus antigen assays may provide veterinarians with a more rapid, patient-side diagnosis when compared with traditional laboratory-based latex agglutination tests. Objective: To determine the sensitivity and specificity of 2 POC lateral flow cryptococcal serum antigen tests, CrAg LFA (Immy, Norman, OK) and the CryptoPS (Biosynex, Strasbourg, France) for diagnosis of cryptococcosis in dogs and cats, using the cryptococcal antigen latex agglutination system (CALAS) as the reference standard. Animals: 102 serum samples from 51 dogs and 40 cats. Methods: Specimens were classified as CALAS-positive (n = 25) or CALAS-negative (n = 77). The sensitivity and specificity of each POC assay was calculated by comparing the results to the serologic reference standard results. Results: The CrAg LFA assay correctly classified 23/25 CALAS-positive specimens and 69/74 CALAS-negative specimens resulting in a sensitivity of 92.0% (confidence interval [CI], 75.0%-98.6%) and specificity of 93.2% (CI, 85.1%-97.1%). The CryptoPS assay correctly classified 8/10 tested CALAS-positive specimens and 56/59 tested CALAS-negative specimens resulting in a sensitivity of 80.0% (CI, 49.0%-96.5%) and specificity of 94.9% (CI, 86.1%-98.6%). Conclusion and Clinical Importance: The POC assays appear to be a sensitive and specific alternative to the traditional CALAS assay with more rapid turnaround times, which may result in earlier diagnosis and treatment.

Original languageEnglish (US)
JournalJournal of Veterinary Internal Medicine
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Point-of-Care Systems
Agglutination
Latex
agglutination
latex
Cats
Dogs
cats
antigens
Antigens
dogs
testing
confidence interval
assays
Confidence Intervals
reference standards
Latex Fixation Tests
latex agglutination test
Cryptococcus
Sensitivity and Specificity

Keywords

  • Cryptococcus
  • diagnostic test
  • fungal

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{ceccf3376c664238bf3c4fc62bf0e063,
title = "Evaluation of the clinical performance of 2 point-of-care cryptococcal antigen tests in dogs and cats",
abstract = "Background: Point-of-care (POC) Cryptococcus antigen assays may provide veterinarians with a more rapid, patient-side diagnosis when compared with traditional laboratory-based latex agglutination tests. Objective: To determine the sensitivity and specificity of 2 POC lateral flow cryptococcal serum antigen tests, CrAg LFA (Immy, Norman, OK) and the CryptoPS (Biosynex, Strasbourg, France) for diagnosis of cryptococcosis in dogs and cats, using the cryptococcal antigen latex agglutination system (CALAS) as the reference standard. Animals: 102 serum samples from 51 dogs and 40 cats. Methods: Specimens were classified as CALAS-positive (n = 25) or CALAS-negative (n = 77). The sensitivity and specificity of each POC assay was calculated by comparing the results to the serologic reference standard results. Results: The CrAg LFA assay correctly classified 23/25 CALAS-positive specimens and 69/74 CALAS-negative specimens resulting in a sensitivity of 92.0{\%} (confidence interval [CI], 75.0{\%}-98.6{\%}) and specificity of 93.2{\%} (CI, 85.1{\%}-97.1{\%}). The CryptoPS assay correctly classified 8/10 tested CALAS-positive specimens and 56/59 tested CALAS-negative specimens resulting in a sensitivity of 80.0{\%} (CI, 49.0{\%}-96.5{\%}) and specificity of 94.9{\%} (CI, 86.1{\%}-98.6{\%}). Conclusion and Clinical Importance: The POC assays appear to be a sensitive and specific alternative to the traditional CALAS assay with more rapid turnaround times, which may result in earlier diagnosis and treatment.",
keywords = "Cryptococcus, diagnostic test, fungal",
author = "Reagan, {Krystle L.} and Mchardy, {Ian Howard} and Thompson, {George Richard} and Sykes, {Jane E}",
year = "2019",
month = "1",
day = "1",
doi = "10.1111/jvim.15599",
language = "English (US)",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Evaluation of the clinical performance of 2 point-of-care cryptococcal antigen tests in dogs and cats

AU - Reagan, Krystle L.

AU - Mchardy, Ian Howard

AU - Thompson, George Richard

AU - Sykes, Jane E

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Point-of-care (POC) Cryptococcus antigen assays may provide veterinarians with a more rapid, patient-side diagnosis when compared with traditional laboratory-based latex agglutination tests. Objective: To determine the sensitivity and specificity of 2 POC lateral flow cryptococcal serum antigen tests, CrAg LFA (Immy, Norman, OK) and the CryptoPS (Biosynex, Strasbourg, France) for diagnosis of cryptococcosis in dogs and cats, using the cryptococcal antigen latex agglutination system (CALAS) as the reference standard. Animals: 102 serum samples from 51 dogs and 40 cats. Methods: Specimens were classified as CALAS-positive (n = 25) or CALAS-negative (n = 77). The sensitivity and specificity of each POC assay was calculated by comparing the results to the serologic reference standard results. Results: The CrAg LFA assay correctly classified 23/25 CALAS-positive specimens and 69/74 CALAS-negative specimens resulting in a sensitivity of 92.0% (confidence interval [CI], 75.0%-98.6%) and specificity of 93.2% (CI, 85.1%-97.1%). The CryptoPS assay correctly classified 8/10 tested CALAS-positive specimens and 56/59 tested CALAS-negative specimens resulting in a sensitivity of 80.0% (CI, 49.0%-96.5%) and specificity of 94.9% (CI, 86.1%-98.6%). Conclusion and Clinical Importance: The POC assays appear to be a sensitive and specific alternative to the traditional CALAS assay with more rapid turnaround times, which may result in earlier diagnosis and treatment.

AB - Background: Point-of-care (POC) Cryptococcus antigen assays may provide veterinarians with a more rapid, patient-side diagnosis when compared with traditional laboratory-based latex agglutination tests. Objective: To determine the sensitivity and specificity of 2 POC lateral flow cryptococcal serum antigen tests, CrAg LFA (Immy, Norman, OK) and the CryptoPS (Biosynex, Strasbourg, France) for diagnosis of cryptococcosis in dogs and cats, using the cryptococcal antigen latex agglutination system (CALAS) as the reference standard. Animals: 102 serum samples from 51 dogs and 40 cats. Methods: Specimens were classified as CALAS-positive (n = 25) or CALAS-negative (n = 77). The sensitivity and specificity of each POC assay was calculated by comparing the results to the serologic reference standard results. Results: The CrAg LFA assay correctly classified 23/25 CALAS-positive specimens and 69/74 CALAS-negative specimens resulting in a sensitivity of 92.0% (confidence interval [CI], 75.0%-98.6%) and specificity of 93.2% (CI, 85.1%-97.1%). The CryptoPS assay correctly classified 8/10 tested CALAS-positive specimens and 56/59 tested CALAS-negative specimens resulting in a sensitivity of 80.0% (CI, 49.0%-96.5%) and specificity of 94.9% (CI, 86.1%-98.6%). Conclusion and Clinical Importance: The POC assays appear to be a sensitive and specific alternative to the traditional CALAS assay with more rapid turnaround times, which may result in earlier diagnosis and treatment.

KW - Cryptococcus

KW - diagnostic test

KW - fungal

UR - http://www.scopus.com/inward/record.url?scp=85071357899&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071357899&partnerID=8YFLogxK

U2 - 10.1111/jvim.15599

DO - 10.1111/jvim.15599

M3 - Article

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

ER -