Evaluation of the agreement among three handheld blood glucose meters and a laboratory blood analyzer for measurement of blood glucose concentration in Hispaniolan Amazon parrots (Amazona ventralis)

Mark J. Acierno, Mark A. Mitchell, Patricia J. Schuster, Diana Freeman, David Guzman, Thomas N. Tully

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective - To determine the degree of agreement between 3 commercially available point-of-care blood glucose meters and a laboratory analyzer for measurement of blood glucose concentrations in Hispaniolan Amazon parrots (Amazona ventralis). Animals - 20 healthy adult Hispaniolan Amazon parrots. Procedures - A 26-gauge needle and 3-mL syringe were used to obtain a blood sample (approx 0.5 mL) from a jugular vein of each parrot. Small volumes of blood (0.6 to 1.5 μL) were used to operate each of the blood glucose meters, and the remainder was placed into lithium heparin microtubes and centrifuged. Plasma was harvested and frozen at -30°C. Within 5 days after collection, plasma samples were thawed and plasma glucose concentrations were measured by means of the laboratory analyzer. Agreement between pairs of blood glucose meters and between each blood glucose meter and the laboratory analyzer was evaluated by means of the Bland-Altman method, and limits of agreement (LOA) were calculated. Results - None of the results of the 3 blood glucose meters agreed with results of the laboratory analyzer. Each point-of-care blood glucose meter underestimated the blood glucose concentration, and the degree of negative bias was not consistent (meter A bias, -94.9 mg/dL [LOA, -148.0 to -41.7 mg/dL]; meter B bias, -52 mg/dL [LOA, -107.5 to 3.5 mg/dL]; and meter C bias, -78.9 mg/dL [LOA, -137.2 to -20.6 mg/dL]). Conclusions and Clinical Relevance - On the basis of these results, use of handheld blood glucose meters in the diagnosis or treatment of Hispaniolan Amazon parrots and other psittacines cannot be recommended.

Original languageEnglish (US)
Pages (from-to)172-175
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume70
Issue number2
DOIs
StatePublished - Feb 1 2009
Externally publishedYes

Fingerprint

Amazona
parrots
meters (equipment)
blood glucose
Blood Glucose
blood
Point-of-Care Systems
Psittaciformes
Parrots
faropenem medoxomil
Jugular Veins
Syringes
blood volume
lithium
syringes
Blood Volume
jugular vein
Lithium
heparin
gauges

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of the agreement among three handheld blood glucose meters and a laboratory blood analyzer for measurement of blood glucose concentration in Hispaniolan Amazon parrots (Amazona ventralis). / Acierno, Mark J.; Mitchell, Mark A.; Schuster, Patricia J.; Freeman, Diana; Guzman, David; Tully, Thomas N.

In: American Journal of Veterinary Research, Vol. 70, No. 2, 01.02.2009, p. 172-175.

Research output: Contribution to journalArticle

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abstract = "Objective - To determine the degree of agreement between 3 commercially available point-of-care blood glucose meters and a laboratory analyzer for measurement of blood glucose concentrations in Hispaniolan Amazon parrots (Amazona ventralis). Animals - 20 healthy adult Hispaniolan Amazon parrots. Procedures - A 26-gauge needle and 3-mL syringe were used to obtain a blood sample (approx 0.5 mL) from a jugular vein of each parrot. Small volumes of blood (0.6 to 1.5 μL) were used to operate each of the blood glucose meters, and the remainder was placed into lithium heparin microtubes and centrifuged. Plasma was harvested and frozen at -30°C. Within 5 days after collection, plasma samples were thawed and plasma glucose concentrations were measured by means of the laboratory analyzer. Agreement between pairs of blood glucose meters and between each blood glucose meter and the laboratory analyzer was evaluated by means of the Bland-Altman method, and limits of agreement (LOA) were calculated. Results - None of the results of the 3 blood glucose meters agreed with results of the laboratory analyzer. Each point-of-care blood glucose meter underestimated the blood glucose concentration, and the degree of negative bias was not consistent (meter A bias, -94.9 mg/dL [LOA, -148.0 to -41.7 mg/dL]; meter B bias, -52 mg/dL [LOA, -107.5 to 3.5 mg/dL]; and meter C bias, -78.9 mg/dL [LOA, -137.2 to -20.6 mg/dL]). Conclusions and Clinical Relevance - On the basis of these results, use of handheld blood glucose meters in the diagnosis or treatment of Hispaniolan Amazon parrots and other psittacines cannot be recommended.",
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