Evaluation of severe disease induced by aerosol inoculation of calves with bovine respiratory syncytial virus

Amelia R. Woolums, Mark L Anderson, Robert A. Gunther, Edward S Schelegle, Danielle R. LaRochelle, Randall S. Singer, Gabrielle A. Boyle, Kathleen E. Friebertshauser, Laurel J Gershwin

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Objective - To develop a model of bovine respiratory syncytial virus (BRSV) infection that induces severe disease similar to that seen in some cattle with naturally acquired BRSV infection. Animals - 25 male Holstein calves, 8 to16 weeks old. Procedure - 17 calves were given a low-passage field isolate of BRSV by aerosolization; 8 control calves were given supernatant from noninfected cell culture. Disease was characterized by evaluating clinical signs, virus isolation and pulmonary function tests, and results of blood gas analysis, gross and histologic postmortem examination, and microbiologic testing. Results - Cumulative incidence of cough, harsh lung sounds, adventitious sounds, and dyspnea and increases in rectal temperature and respiratory rate were significantly greater in infected calves. Three infected calves developed extreme respiratory distress and were euthanatized 7 days after inoculation. Virus was isolated from nasal swab specimens from all infected calves but not from mock infected calves. On day 7 after inoculation, mean PaO2 and PaCO2 were significantly lower, and pulmonary resistance was significantly higher, in infected calves. During necropsy, infected calves had varying degrees of necrotizing and proliferate bronchiolitis and alveolitis with syncytial formation. The 3 calves euthanatized on day 7 had emphysematous bullae in the caudal lung lobes; 1 had unilateral pneumothorax. Conclusion and Clinical Relevance - Severe disease similar to that seen in some cattle with naturally acquired BRSV infection can be induced in calves with a single aerosol exposure of a low-passage clinical isolate of BRSV. Our model will be useful for studying the pathogenesis of BRSV infection and for evaluating vaccines and therapeutics.

Original languageEnglish (US)
Pages (from-to)473-480
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume60
Issue number4
StatePublished - Apr 1999

Fingerprint

Bovine respiratory syncytial virus
aerosols
Aerosols
Respiratory Syncytial Virus Infections
vaccination
calves
Viruses
Lung
Blood Gas Analysis
lungs
Bronchiolitis
Respiratory Function Tests
Respiratory Sounds
Pneumothorax
Blister
Respiratory Rate
infection
Nose
Cough
Dyspnea

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of severe disease induced by aerosol inoculation of calves with bovine respiratory syncytial virus. / Woolums, Amelia R.; Anderson, Mark L; Gunther, Robert A.; Schelegle, Edward S; LaRochelle, Danielle R.; Singer, Randall S.; Boyle, Gabrielle A.; Friebertshauser, Kathleen E.; Gershwin, Laurel J.

In: American Journal of Veterinary Research, Vol. 60, No. 4, 04.1999, p. 473-480.

Research output: Contribution to journalArticle

Woolums, Amelia R. ; Anderson, Mark L ; Gunther, Robert A. ; Schelegle, Edward S ; LaRochelle, Danielle R. ; Singer, Randall S. ; Boyle, Gabrielle A. ; Friebertshauser, Kathleen E. ; Gershwin, Laurel J. / Evaluation of severe disease induced by aerosol inoculation of calves with bovine respiratory syncytial virus. In: American Journal of Veterinary Research. 1999 ; Vol. 60, No. 4. pp. 473-480.
@article{94f568bae8e8425683c3f6c63a34f867,
title = "Evaluation of severe disease induced by aerosol inoculation of calves with bovine respiratory syncytial virus",
abstract = "Objective - To develop a model of bovine respiratory syncytial virus (BRSV) infection that induces severe disease similar to that seen in some cattle with naturally acquired BRSV infection. Animals - 25 male Holstein calves, 8 to16 weeks old. Procedure - 17 calves were given a low-passage field isolate of BRSV by aerosolization; 8 control calves were given supernatant from noninfected cell culture. Disease was characterized by evaluating clinical signs, virus isolation and pulmonary function tests, and results of blood gas analysis, gross and histologic postmortem examination, and microbiologic testing. Results - Cumulative incidence of cough, harsh lung sounds, adventitious sounds, and dyspnea and increases in rectal temperature and respiratory rate were significantly greater in infected calves. Three infected calves developed extreme respiratory distress and were euthanatized 7 days after inoculation. Virus was isolated from nasal swab specimens from all infected calves but not from mock infected calves. On day 7 after inoculation, mean PaO2 and PaCO2 were significantly lower, and pulmonary resistance was significantly higher, in infected calves. During necropsy, infected calves had varying degrees of necrotizing and proliferate bronchiolitis and alveolitis with syncytial formation. The 3 calves euthanatized on day 7 had emphysematous bullae in the caudal lung lobes; 1 had unilateral pneumothorax. Conclusion and Clinical Relevance - Severe disease similar to that seen in some cattle with naturally acquired BRSV infection can be induced in calves with a single aerosol exposure of a low-passage clinical isolate of BRSV. Our model will be useful for studying the pathogenesis of BRSV infection and for evaluating vaccines and therapeutics.",
author = "Woolums, {Amelia R.} and Anderson, {Mark L} and Gunther, {Robert A.} and Schelegle, {Edward S} and LaRochelle, {Danielle R.} and Singer, {Randall S.} and Boyle, {Gabrielle A.} and Friebertshauser, {Kathleen E.} and Gershwin, {Laurel J}",
year = "1999",
month = "4",
language = "English (US)",
volume = "60",
pages = "473--480",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "4",

}

TY - JOUR

T1 - Evaluation of severe disease induced by aerosol inoculation of calves with bovine respiratory syncytial virus

AU - Woolums, Amelia R.

AU - Anderson, Mark L

AU - Gunther, Robert A.

AU - Schelegle, Edward S

AU - LaRochelle, Danielle R.

AU - Singer, Randall S.

AU - Boyle, Gabrielle A.

AU - Friebertshauser, Kathleen E.

AU - Gershwin, Laurel J

PY - 1999/4

Y1 - 1999/4

N2 - Objective - To develop a model of bovine respiratory syncytial virus (BRSV) infection that induces severe disease similar to that seen in some cattle with naturally acquired BRSV infection. Animals - 25 male Holstein calves, 8 to16 weeks old. Procedure - 17 calves were given a low-passage field isolate of BRSV by aerosolization; 8 control calves were given supernatant from noninfected cell culture. Disease was characterized by evaluating clinical signs, virus isolation and pulmonary function tests, and results of blood gas analysis, gross and histologic postmortem examination, and microbiologic testing. Results - Cumulative incidence of cough, harsh lung sounds, adventitious sounds, and dyspnea and increases in rectal temperature and respiratory rate were significantly greater in infected calves. Three infected calves developed extreme respiratory distress and were euthanatized 7 days after inoculation. Virus was isolated from nasal swab specimens from all infected calves but not from mock infected calves. On day 7 after inoculation, mean PaO2 and PaCO2 were significantly lower, and pulmonary resistance was significantly higher, in infected calves. During necropsy, infected calves had varying degrees of necrotizing and proliferate bronchiolitis and alveolitis with syncytial formation. The 3 calves euthanatized on day 7 had emphysematous bullae in the caudal lung lobes; 1 had unilateral pneumothorax. Conclusion and Clinical Relevance - Severe disease similar to that seen in some cattle with naturally acquired BRSV infection can be induced in calves with a single aerosol exposure of a low-passage clinical isolate of BRSV. Our model will be useful for studying the pathogenesis of BRSV infection and for evaluating vaccines and therapeutics.

AB - Objective - To develop a model of bovine respiratory syncytial virus (BRSV) infection that induces severe disease similar to that seen in some cattle with naturally acquired BRSV infection. Animals - 25 male Holstein calves, 8 to16 weeks old. Procedure - 17 calves were given a low-passage field isolate of BRSV by aerosolization; 8 control calves were given supernatant from noninfected cell culture. Disease was characterized by evaluating clinical signs, virus isolation and pulmonary function tests, and results of blood gas analysis, gross and histologic postmortem examination, and microbiologic testing. Results - Cumulative incidence of cough, harsh lung sounds, adventitious sounds, and dyspnea and increases in rectal temperature and respiratory rate were significantly greater in infected calves. Three infected calves developed extreme respiratory distress and were euthanatized 7 days after inoculation. Virus was isolated from nasal swab specimens from all infected calves but not from mock infected calves. On day 7 after inoculation, mean PaO2 and PaCO2 were significantly lower, and pulmonary resistance was significantly higher, in infected calves. During necropsy, infected calves had varying degrees of necrotizing and proliferate bronchiolitis and alveolitis with syncytial formation. The 3 calves euthanatized on day 7 had emphysematous bullae in the caudal lung lobes; 1 had unilateral pneumothorax. Conclusion and Clinical Relevance - Severe disease similar to that seen in some cattle with naturally acquired BRSV infection can be induced in calves with a single aerosol exposure of a low-passage clinical isolate of BRSV. Our model will be useful for studying the pathogenesis of BRSV infection and for evaluating vaccines and therapeutics.

UR - http://www.scopus.com/inward/record.url?scp=0033111466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033111466&partnerID=8YFLogxK

M3 - Article

VL - 60

SP - 473

EP - 480

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 4

ER -