Evaluation of serial blood lactate and the use of a point-of-care lactate meter in live-stranded pinnipeds

Matt Marinkovich, Raymund F. Wack, Cara L. Field, Sophie T. Whoriskey, Philip H Kass, Jenessa Gjeltema

Research output: Contribution to journalArticle

Abstract

Live-stranded pinnipeds often present to rehabilitation centers systemically debilitated with dehydration and poor perfusion. In many terrestrial mammals, blood lactate elevation has been correlated with global tissue hypoxia and decreased circulating blood volume. Serial blood lactate measurements in companion animals and humans have been used to guide fluid resuscitation therapy and evaluate prognosis. The primary objective of this study was to evaluate the prognostic value of serial blood lactate levels in live-stranded pinnipeds in a rehabilitation setting. The secondary objectives were to evaluate the use of a point-of-care lactate meter, and potassium oxalate-sodium fluoride (gray-top) tubes for storing samples for lactate analysis in pinniped patients. Fifty-five live-stranded pinnipeds (30 northern elephant seals [Mirounga angustirostris], 21 Pacific harbor seals [Phoca vitulina richardsi], and four California sea lions [Zalophus californianus]) that presented to a rehabilitation center were manually restrained for blood collection and lactate measurement using a point-of-care analyzer (Lactate Plus ™) prior to fluid or other medical therapy. Lactate measurements were repeated 72 hr post admission and prior to euthanasia or release. Plasma samples from gray-top tubes were frozen for 30-100 days at -80°C, then thawed and evaluated using both the LactatePlus and bench-top (ABL 815) analyzers. The point-of-care analyzer was successful in measuring lactate in pinnipeds. Gray-top tubes were effective at preserving lactate levels in frozen plasma samples for up to 100 days. Released animals had significantly greater lactate clearance at 72 hr (P = 0.039) than animals that died or were euthanized. Therefore, lactate clearance, determined by serial blood lactate measurements, may be useful for evaluating prognosis in live-stranded pinnipeds. Initial lactate and lactate values prior to euthanasia or release were not significantly associated with outcome. Given these findings, clinicians should interpret isolated lactate values in pinnipeds with caution.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalJournal of Zoo and Wildlife Medicine
Volume50
Issue number1
DOIs
StatePublished - Mar 1 2019

Fingerprint

Pinnipedia
Point-of-Care Systems
lactates
Lactic Acid
blood
rehabilitation (people)
Sea Lions
Phoca
Earless Seals
Mirounga angustirostris
Rehabilitation Centers
Phoca vitulina
Euthanasia
euthanasia
prognosis
Oxalic Acid
sodium fluoride
Sodium Fluoride
therapeutics

Keywords

  • Lactate clearance
  • LactatePlus
  • Mirounga angustirostris
  • Phoca vitulina richardsi
  • Pinniped rehabilitation
  • Zalophus californianus

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Evaluation of serial blood lactate and the use of a point-of-care lactate meter in live-stranded pinnipeds. / Marinkovich, Matt; Wack, Raymund F.; Field, Cara L.; Whoriskey, Sophie T.; Kass, Philip H; Gjeltema, Jenessa.

In: Journal of Zoo and Wildlife Medicine, Vol. 50, No. 1, 01.03.2019, p. 137-146.

Research output: Contribution to journalArticle

Marinkovich, Matt ; Wack, Raymund F. ; Field, Cara L. ; Whoriskey, Sophie T. ; Kass, Philip H ; Gjeltema, Jenessa. / Evaluation of serial blood lactate and the use of a point-of-care lactate meter in live-stranded pinnipeds. In: Journal of Zoo and Wildlife Medicine. 2019 ; Vol. 50, No. 1. pp. 137-146.
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