Evaluation of platelet function using multiple electrode platelet aggregometry in dogs with septic peritonitis

Ronald H L Li, Daniel L. Chan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To assess platelet function via multiple electrode platelet aggregometry (MEPA) in dogs with septic peritonitis and in healthy dogs. The secondary aim was to determine if there is prognostic significance to changes in platelet function observed in septic dogs. Design: Prospective, observational cohort study conducted from January 2012 to March 2014. Setting: University teaching hospital. Animals: Twenty dogs with septic peritonitis and 23 healthy dogs. Interventions: None. Measurements and Main Results: MEPA using arachidonic acid, adenosine diphosphate, and collagen (COL) as agonists was measured within 24 hours of diagnosis of sepsis. Compared to healthy dogs, platelet aggregation was reduced in dogs with septic peritonitis for all agonists (P < 0.01). Overall mortality rate was 40%. MEPA in response to COL was significantly reduced in nonsurvivors compared to survivors (P = 0.019). Using receiver-operating characteristic curve statistics, a COL-activated MEPA less than 43.5 aggregation units had a sensitivity and specificity of 85.7% and 90.9%, respectively, for predicting nonsurvival in dogs with septic peritonitis. Conclusions: Circulating platelets from dogs with septic peritonitis have diminished aggregation in response to multiple platelet agonists. MEPA may serve as an assessment tool for illness severity in this patient population.

Original languageEnglish (US)
Pages (from-to)630-638
Number of pages9
JournalJournal of Veterinary Emergency and Critical Care
Volume26
Issue number5
DOIs
StatePublished - Sep 1 2016

Fingerprint

peritonitis
Peritonitis
electrodes
Electrodes
Blood Platelets
Dogs
dogs
agonists
collagen
Collagen
adenosine diphosphate
platelet aggregation
cohort studies
arachidonic acid
Platelet Aggregation
Arachidonic Acid
ROC Curve
Teaching Hospitals
Adenosine Diphosphate
Observational Studies

Keywords

  • canine
  • hemostasis
  • multiple organ dysfunction syndrome
  • platelet dysfunction
  • sepsis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of platelet function using multiple electrode platelet aggregometry in dogs with septic peritonitis. / Li, Ronald H L; Chan, Daniel L.

In: Journal of Veterinary Emergency and Critical Care, Vol. 26, No. 5, 01.09.2016, p. 630-638.

Research output: Contribution to journalArticle

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