Evaluation of plasma (1→3) β-D-glucan concentrations in birds naturally and experimentally infected with aspergillus fumigatus

Julia D. Burco, Michael H Ziccardi, Karl V. Clemons, Lisa A Tell

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Avian aspergillosis, most often caused by Aspergillus fumigatus, is a common and devastating disease affecting a range of bird species. Early diagnosis is difficult and often unreliable. The current study evaluated the utility of measuring (1→3)-β-D-glucan (BG) concentrations in avian plasma samples to aid in the diagnosis of aspergillosis. We evaluated a commercially available BG assay (Fungitell®, Beacon Diagnostics) using 178 plasma samples from naturally infected, experimentally infected, and aspergillosis-free birds. Although there was variation in BG concentration, as reflected by high standard deviations, seabirds with confirmed aspergillosis had the highest mean BG concentrations (M = 3098.7 pg/dl, SD = 5022.6, n = 22) followed by companion avian species and raptors with confirmed aspergillosis (M =1033.8 pg/dl, SD = 1531.6, n = 19) and experimentally infected Japanese quail (Coturnix japonica; M = 1066.5 pg/dl, SD = 1348.2, n = 17). Variation in severity of disease, differences among species of birds with and without disease, and also different levels in environmental exposure likely contribute to the differences among avian groups. The overall sensitivity and specificity of the BG test for diagnosis of aspergillosis in birds was 60.0 and 92.7%, respectively, with an overall optimized avian cut-off value of ≥461 pg/dl for positive disease. Our findings suggest that, although BG concentrations are highly variable between and within different avian groups, it could serve as a useful adjunctive diagnostic test for aspergillosis that is applicable to multiple avian species in some settings, particularly as a negative predictor of infection.

Original languageEnglish (US)
Pages (from-to)183-191
Number of pages9
JournalAvian Diseases
Volume56
Issue number1
DOIs
StatePublished - Mar 2012

Fingerprint

Aspergillus fumigatus
Aspergillosis
aspergillosis
glucans
Birds
birds
Coturnix
Raptors
early diagnosis
Environmental Exposure
birds of prey
polyglucosan
seabirds
interspecific variation
Routine Diagnostic Tests
diagnostic techniques
disease severity
Early Diagnosis
Sensitivity and Specificity
sampling

Keywords

  • β-D-glucan
  • aspergillosis
  • avian
  • Fungitell assay

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Immunology and Microbiology(all)

Cite this

Evaluation of plasma (1→3) β-D-glucan concentrations in birds naturally and experimentally infected with aspergillus fumigatus. / Burco, Julia D.; Ziccardi, Michael H; Clemons, Karl V.; Tell, Lisa A.

In: Avian Diseases, Vol. 56, No. 1, 03.2012, p. 183-191.

Research output: Contribution to journalArticle

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abstract = "Avian aspergillosis, most often caused by Aspergillus fumigatus, is a common and devastating disease affecting a range of bird species. Early diagnosis is difficult and often unreliable. The current study evaluated the utility of measuring (1→3)-β-D-glucan (BG) concentrations in avian plasma samples to aid in the diagnosis of aspergillosis. We evaluated a commercially available BG assay (Fungitell{\circledR}, Beacon Diagnostics) using 178 plasma samples from naturally infected, experimentally infected, and aspergillosis-free birds. Although there was variation in BG concentration, as reflected by high standard deviations, seabirds with confirmed aspergillosis had the highest mean BG concentrations (M = 3098.7 pg/dl, SD = 5022.6, n = 22) followed by companion avian species and raptors with confirmed aspergillosis (M =1033.8 pg/dl, SD = 1531.6, n = 19) and experimentally infected Japanese quail (Coturnix japonica; M = 1066.5 pg/dl, SD = 1348.2, n = 17). Variation in severity of disease, differences among species of birds with and without disease, and also different levels in environmental exposure likely contribute to the differences among avian groups. The overall sensitivity and specificity of the BG test for diagnosis of aspergillosis in birds was 60.0 and 92.7{\%}, respectively, with an overall optimized avian cut-off value of ≥461 pg/dl for positive disease. Our findings suggest that, although BG concentrations are highly variable between and within different avian groups, it could serve as a useful adjunctive diagnostic test for aspergillosis that is applicable to multiple avian species in some settings, particularly as a negative predictor of infection.",
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