Evaluation of periparturient dairy cows and contact surfaces as a reservoir of Cryptosporidium parvum for calfhood infection

Edward R Atwill, James A. Harp, Ted Jones, Phillip W. Jardon, Stephanie Checel, Mike Zylstra

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Objective - To determine whether periparturient cows or contact surfaces to which newborn calves are exposed are reservoirs of Cryptosporidium parvum oocysts. Animals - Periparturient cows and their calves. Procedure - Using direct fluorescent antibody (DFA) and acid-fast (AF) assays, fecal samples taken before and after calving from periparturient cows were tested for C parvum oocysts. Fecal samples from calves were collected every other day from age 7 to 21 days and were tested by use of the AF assay. Topsoil from close-up and maternity pens and scrapings from wooden walls and floors of calf hutches were tested for C parvum oocysts by use of DFA assay. Results - None of the 384 fecal samples obtained 1 to 21 days before or after calving or on the day of calving from 154 periparturient cows contained detectable C parvum oocysts. Despite this lack of detectable periparturient shedding, the period prevalence of calfhood infection was 92% (123/134) from age 7 to 21 days. Soil samples from the close-up and maternity pens where newborn calves spend the first 12 hours of life also were negative for C parvum oocysts. Wood scrapings from the outer 2 mm of the walls and floors of empty and cleaned calf hutches that were ready to receive calves were C parvum oocyst-positive. Conclusions - Conditional on sensitivity of DFA, periparturient cows did not appear to shed detectable C parvum oocysts. In contrast, the floors and walls of wooden calf hutches contained detectable C parvum oocysts on the surface.

Original languageEnglish (US)
Pages (from-to)1116-1121
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume59
Issue number9
StatePublished - Sep 1998

Fingerprint

Cryptosporidium parvum
Oocysts
oocysts
dairy cows
calves
Infection
infection
cows
calving
antibodies
Antibodies
neonates
assays
Acids
acids
sampling
topsoil
Soil
soil sampling

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of periparturient dairy cows and contact surfaces as a reservoir of Cryptosporidium parvum for calfhood infection. / Atwill, Edward R; Harp, James A.; Jones, Ted; Jardon, Phillip W.; Checel, Stephanie; Zylstra, Mike.

In: American Journal of Veterinary Research, Vol. 59, No. 9, 09.1998, p. 1116-1121.

Research output: Contribution to journalArticle

Atwill, Edward R ; Harp, James A. ; Jones, Ted ; Jardon, Phillip W. ; Checel, Stephanie ; Zylstra, Mike. / Evaluation of periparturient dairy cows and contact surfaces as a reservoir of Cryptosporidium parvum for calfhood infection. In: American Journal of Veterinary Research. 1998 ; Vol. 59, No. 9. pp. 1116-1121.
@article{e246795e0fd64a64a7c0780ad4265c31,
title = "Evaluation of periparturient dairy cows and contact surfaces as a reservoir of Cryptosporidium parvum for calfhood infection",
abstract = "Objective - To determine whether periparturient cows or contact surfaces to which newborn calves are exposed are reservoirs of Cryptosporidium parvum oocysts. Animals - Periparturient cows and their calves. Procedure - Using direct fluorescent antibody (DFA) and acid-fast (AF) assays, fecal samples taken before and after calving from periparturient cows were tested for C parvum oocysts. Fecal samples from calves were collected every other day from age 7 to 21 days and were tested by use of the AF assay. Topsoil from close-up and maternity pens and scrapings from wooden walls and floors of calf hutches were tested for C parvum oocysts by use of DFA assay. Results - None of the 384 fecal samples obtained 1 to 21 days before or after calving or on the day of calving from 154 periparturient cows contained detectable C parvum oocysts. Despite this lack of detectable periparturient shedding, the period prevalence of calfhood infection was 92{\%} (123/134) from age 7 to 21 days. Soil samples from the close-up and maternity pens where newborn calves spend the first 12 hours of life also were negative for C parvum oocysts. Wood scrapings from the outer 2 mm of the walls and floors of empty and cleaned calf hutches that were ready to receive calves were C parvum oocyst-positive. Conclusions - Conditional on sensitivity of DFA, periparturient cows did not appear to shed detectable C parvum oocysts. In contrast, the floors and walls of wooden calf hutches contained detectable C parvum oocysts on the surface.",
author = "Atwill, {Edward R} and Harp, {James A.} and Ted Jones and Jardon, {Phillip W.} and Stephanie Checel and Mike Zylstra",
year = "1998",
month = "9",
language = "English (US)",
volume = "59",
pages = "1116--1121",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "9",

}

TY - JOUR

T1 - Evaluation of periparturient dairy cows and contact surfaces as a reservoir of Cryptosporidium parvum for calfhood infection

AU - Atwill, Edward R

AU - Harp, James A.

AU - Jones, Ted

AU - Jardon, Phillip W.

AU - Checel, Stephanie

AU - Zylstra, Mike

PY - 1998/9

Y1 - 1998/9

N2 - Objective - To determine whether periparturient cows or contact surfaces to which newborn calves are exposed are reservoirs of Cryptosporidium parvum oocysts. Animals - Periparturient cows and their calves. Procedure - Using direct fluorescent antibody (DFA) and acid-fast (AF) assays, fecal samples taken before and after calving from periparturient cows were tested for C parvum oocysts. Fecal samples from calves were collected every other day from age 7 to 21 days and were tested by use of the AF assay. Topsoil from close-up and maternity pens and scrapings from wooden walls and floors of calf hutches were tested for C parvum oocysts by use of DFA assay. Results - None of the 384 fecal samples obtained 1 to 21 days before or after calving or on the day of calving from 154 periparturient cows contained detectable C parvum oocysts. Despite this lack of detectable periparturient shedding, the period prevalence of calfhood infection was 92% (123/134) from age 7 to 21 days. Soil samples from the close-up and maternity pens where newborn calves spend the first 12 hours of life also were negative for C parvum oocysts. Wood scrapings from the outer 2 mm of the walls and floors of empty and cleaned calf hutches that were ready to receive calves were C parvum oocyst-positive. Conclusions - Conditional on sensitivity of DFA, periparturient cows did not appear to shed detectable C parvum oocysts. In contrast, the floors and walls of wooden calf hutches contained detectable C parvum oocysts on the surface.

AB - Objective - To determine whether periparturient cows or contact surfaces to which newborn calves are exposed are reservoirs of Cryptosporidium parvum oocysts. Animals - Periparturient cows and their calves. Procedure - Using direct fluorescent antibody (DFA) and acid-fast (AF) assays, fecal samples taken before and after calving from periparturient cows were tested for C parvum oocysts. Fecal samples from calves were collected every other day from age 7 to 21 days and were tested by use of the AF assay. Topsoil from close-up and maternity pens and scrapings from wooden walls and floors of calf hutches were tested for C parvum oocysts by use of DFA assay. Results - None of the 384 fecal samples obtained 1 to 21 days before or after calving or on the day of calving from 154 periparturient cows contained detectable C parvum oocysts. Despite this lack of detectable periparturient shedding, the period prevalence of calfhood infection was 92% (123/134) from age 7 to 21 days. Soil samples from the close-up and maternity pens where newborn calves spend the first 12 hours of life also were negative for C parvum oocysts. Wood scrapings from the outer 2 mm of the walls and floors of empty and cleaned calf hutches that were ready to receive calves were C parvum oocyst-positive. Conclusions - Conditional on sensitivity of DFA, periparturient cows did not appear to shed detectable C parvum oocysts. In contrast, the floors and walls of wooden calf hutches contained detectable C parvum oocysts on the surface.

UR - http://www.scopus.com/inward/record.url?scp=0032161678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032161678&partnerID=8YFLogxK

M3 - Article

VL - 59

SP - 1116

EP - 1121

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 9

ER -