Evaluation of north American antibody detection tests for diagnosis of brucellosis in goats

Andrea B. Mikolon, Ian Gardner, Sharon K. Hietala, Jorge Hernandez De Anda, Elpidio Chamizo Pestaña, Steven G. Hennager, Anita J. Edmondson

Research output: Contribution to journalArticle

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Abstract

The sensitivities and specificities of 17 antibody detection tests for brucellosis in goats were estimated. Tests evaluated included the U.S. Department of Agriculture (USDA) card test with 8% cell concentration (8%Card), USDA rapid automated presumptive test (RAP), Mexican rose bengal plate tests with 8 and 3% cell concentrations (8%RB and 3%RB), French rose bengal plate test with 4.5% cell concentration (4.5%RB), USDA standard plate test (SPT), USDA buffered acidified plate agglutination test (BAPA), USDA and Mexican rivanol tests (URIV and MRIV), USDA standard tube tests with Brucella abortus and Brucella melitensis antigens (SATA and SATM), serum enzyme- linked immunosorbent assay (ELISA), USDA cold-fixation complement fixation tests with B. abortus and B. melitensis antigens (CFA and CFM), USDA and Mexican milk ring tests (UBRT and MBRT), and a milk ELISA. Test sensitivity was evaluated by using two groups of 10 goats experimentally infected with B. melitensis or B. abortus and monitored for 24 weeks. Specificity was evaluated by using 200 brucellosis-free nonvaccinated goats from 10 California herds. The 3%RB was considered a good screening test because of high sensitivity at week 24 postinfection (90%), ease of performance, and low cost. The cold-fixation CFA and CFM had 100% specificity in the field study and were considered appropriate confirmatory tests. The milk ELISA was significantly more sensitive (P < 0.05) than the UBRT and significantly more specific (P < 0.05) than the MBRT. The milk ELISA also had the advantage of objectivity and ease of interpretation.

Original languageEnglish (US)
Pages (from-to)1716-1722
Number of pages7
JournalJournal of Clinical Microbiology
Volume36
Issue number6
StatePublished - Jun 1 1998

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United States Department of Agriculture
Brucellosis
Goats
Antibodies
Brucella melitensis
Brucella abortus
Milk
Enzyme-Linked Immunosorbent Assay
Rose Bengal
Ethacridine
Antigens
Complement Fixation Tests
Agglutination Tests
Costs and Cost Analysis
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Mikolon, A. B., Gardner, I., Hietala, S. K., Hernandez De Anda, J., Chamizo Pestaña, E., Hennager, S. G., & Edmondson, A. J. (1998). Evaluation of north American antibody detection tests for diagnosis of brucellosis in goats. Journal of Clinical Microbiology, 36(6), 1716-1722.

Evaluation of north American antibody detection tests for diagnosis of brucellosis in goats. / Mikolon, Andrea B.; Gardner, Ian; Hietala, Sharon K.; Hernandez De Anda, Jorge; Chamizo Pestaña, Elpidio; Hennager, Steven G.; Edmondson, Anita J.

In: Journal of Clinical Microbiology, Vol. 36, No. 6, 01.06.1998, p. 1716-1722.

Research output: Contribution to journalArticle

Mikolon, AB, Gardner, I, Hietala, SK, Hernandez De Anda, J, Chamizo Pestaña, E, Hennager, SG & Edmondson, AJ 1998, 'Evaluation of north American antibody detection tests for diagnosis of brucellosis in goats', Journal of Clinical Microbiology, vol. 36, no. 6, pp. 1716-1722.
Mikolon AB, Gardner I, Hietala SK, Hernandez De Anda J, Chamizo Pestaña E, Hennager SG et al. Evaluation of north American antibody detection tests for diagnosis of brucellosis in goats. Journal of Clinical Microbiology. 1998 Jun 1;36(6):1716-1722.
Mikolon, Andrea B. ; Gardner, Ian ; Hietala, Sharon K. ; Hernandez De Anda, Jorge ; Chamizo Pestaña, Elpidio ; Hennager, Steven G. ; Edmondson, Anita J. / Evaluation of north American antibody detection tests for diagnosis of brucellosis in goats. In: Journal of Clinical Microbiology. 1998 ; Vol. 36, No. 6. pp. 1716-1722.
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