Evaluation of neutrophil gelatinase-associated lipocalin as a marker of kidney injury in dogs

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Background: Acute kidney injury (AKI) is a common and often fatal disorder in dogs. Hypothesis: Urine neutrophil gelatinase-associated lipocalin (NGAL)/creatinine ratio is a sensitive and specific biomarker of AKI in dogs. Animals: Ninety-four dogs. Methods: Prospective study. Dogs were classified as follows: (1) healthy dogs, (2) dogs with lower urinary tract disorders, (3) dogs with chronic kidney disease (CKD), (4) dogs with azotemic International Renal Interest Society (IRIS) AKI Grades II-V, and (5) dogs with IRIS AKI Grade I (nonazotemic). Urinary NGAL was quantitated in each dog using an ELISA assay and concentrations were expressed as a ratio to urinary creatinine concentration from the same specimen, and designated the urinary NGAL/creatinine ratio (UNCR). Results: There was a significant difference in UNCR among the study groups (P < .001). Both the azotemic and nonazotemic AKI groups had higher UNCR when compared with all other groups (P < .001 for all pairs). There was a statistically significant difference in UNCR between dogs diagnosed with CKD compared with dogs with lower urinary tract diseases (P = .005) as well as between dogs with CKD and healthy dogs (P = .001). Receiver operator characteristics (ROC) analysis of UNCR as an indicator of azotemic and nonazotemic AKI had an area under the ROC curve of 0.94 and 0.96, respectively. Conclusions and Clinical Relevance: NGAL/creatinine ratio is a sensitive and specific marker of AKI. It can be used to screen patients at risk for AKI and can be utilized to diagnose milder forms of AKI potentially earlier in the course of the disease.

Original languageEnglish (US)
Pages (from-to)1362-1367
Number of pages6
JournalJournal of Veterinary Internal Medicine
Volume27
Issue number6
DOIs
StatePublished - Nov 2013

Fingerprint

neutrophils
kidneys
Dogs
Kidney
Acute Kidney Injury
dogs
Wounds and Injuries
creatinine
Creatinine
kidney diseases
Chronic Renal Insufficiency
urinary tract diseases
Lipocalin-2
Urologic Diseases
prospective studies
Urinary Tract
disease course
biomarkers
urine
Biomarkers

Keywords

  • Acute kidney injury
  • Canine
  • Chronic kidney disease
  • Survival
  • Urinary biomarker

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{8f8d9da6e4a3487ba019cb185ec18edf,
title = "Evaluation of neutrophil gelatinase-associated lipocalin as a marker of kidney injury in dogs",
abstract = "Background: Acute kidney injury (AKI) is a common and often fatal disorder in dogs. Hypothesis: Urine neutrophil gelatinase-associated lipocalin (NGAL)/creatinine ratio is a sensitive and specific biomarker of AKI in dogs. Animals: Ninety-four dogs. Methods: Prospective study. Dogs were classified as follows: (1) healthy dogs, (2) dogs with lower urinary tract disorders, (3) dogs with chronic kidney disease (CKD), (4) dogs with azotemic International Renal Interest Society (IRIS) AKI Grades II-V, and (5) dogs with IRIS AKI Grade I (nonazotemic). Urinary NGAL was quantitated in each dog using an ELISA assay and concentrations were expressed as a ratio to urinary creatinine concentration from the same specimen, and designated the urinary NGAL/creatinine ratio (UNCR). Results: There was a significant difference in UNCR among the study groups (P < .001). Both the azotemic and nonazotemic AKI groups had higher UNCR when compared with all other groups (P < .001 for all pairs). There was a statistically significant difference in UNCR between dogs diagnosed with CKD compared with dogs with lower urinary tract diseases (P = .005) as well as between dogs with CKD and healthy dogs (P = .001). Receiver operator characteristics (ROC) analysis of UNCR as an indicator of azotemic and nonazotemic AKI had an area under the ROC curve of 0.94 and 0.96, respectively. Conclusions and Clinical Relevance: NGAL/creatinine ratio is a sensitive and specific marker of AKI. It can be used to screen patients at risk for AKI and can be utilized to diagnose milder forms of AKI potentially earlier in the course of the disease.",
keywords = "Acute kidney injury, Canine, Chronic kidney disease, Survival, Urinary biomarker",
author = "G. Segev and Carrie Palm and B. Leroy and Cowgill, {Larry D} and Westropp, {Joellen L}",
year = "2013",
month = "11",
doi = "10.1111/jvim.12180",
language = "English (US)",
volume = "27",
pages = "1362--1367",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Evaluation of neutrophil gelatinase-associated lipocalin as a marker of kidney injury in dogs

AU - Segev, G.

AU - Palm, Carrie

AU - Leroy, B.

AU - Cowgill, Larry D

AU - Westropp, Joellen L

PY - 2013/11

Y1 - 2013/11

N2 - Background: Acute kidney injury (AKI) is a common and often fatal disorder in dogs. Hypothesis: Urine neutrophil gelatinase-associated lipocalin (NGAL)/creatinine ratio is a sensitive and specific biomarker of AKI in dogs. Animals: Ninety-four dogs. Methods: Prospective study. Dogs were classified as follows: (1) healthy dogs, (2) dogs with lower urinary tract disorders, (3) dogs with chronic kidney disease (CKD), (4) dogs with azotemic International Renal Interest Society (IRIS) AKI Grades II-V, and (5) dogs with IRIS AKI Grade I (nonazotemic). Urinary NGAL was quantitated in each dog using an ELISA assay and concentrations were expressed as a ratio to urinary creatinine concentration from the same specimen, and designated the urinary NGAL/creatinine ratio (UNCR). Results: There was a significant difference in UNCR among the study groups (P < .001). Both the azotemic and nonazotemic AKI groups had higher UNCR when compared with all other groups (P < .001 for all pairs). There was a statistically significant difference in UNCR between dogs diagnosed with CKD compared with dogs with lower urinary tract diseases (P = .005) as well as between dogs with CKD and healthy dogs (P = .001). Receiver operator characteristics (ROC) analysis of UNCR as an indicator of azotemic and nonazotemic AKI had an area under the ROC curve of 0.94 and 0.96, respectively. Conclusions and Clinical Relevance: NGAL/creatinine ratio is a sensitive and specific marker of AKI. It can be used to screen patients at risk for AKI and can be utilized to diagnose milder forms of AKI potentially earlier in the course of the disease.

AB - Background: Acute kidney injury (AKI) is a common and often fatal disorder in dogs. Hypothesis: Urine neutrophil gelatinase-associated lipocalin (NGAL)/creatinine ratio is a sensitive and specific biomarker of AKI in dogs. Animals: Ninety-four dogs. Methods: Prospective study. Dogs were classified as follows: (1) healthy dogs, (2) dogs with lower urinary tract disorders, (3) dogs with chronic kidney disease (CKD), (4) dogs with azotemic International Renal Interest Society (IRIS) AKI Grades II-V, and (5) dogs with IRIS AKI Grade I (nonazotemic). Urinary NGAL was quantitated in each dog using an ELISA assay and concentrations were expressed as a ratio to urinary creatinine concentration from the same specimen, and designated the urinary NGAL/creatinine ratio (UNCR). Results: There was a significant difference in UNCR among the study groups (P < .001). Both the azotemic and nonazotemic AKI groups had higher UNCR when compared with all other groups (P < .001 for all pairs). There was a statistically significant difference in UNCR between dogs diagnosed with CKD compared with dogs with lower urinary tract diseases (P = .005) as well as between dogs with CKD and healthy dogs (P = .001). Receiver operator characteristics (ROC) analysis of UNCR as an indicator of azotemic and nonazotemic AKI had an area under the ROC curve of 0.94 and 0.96, respectively. Conclusions and Clinical Relevance: NGAL/creatinine ratio is a sensitive and specific marker of AKI. It can be used to screen patients at risk for AKI and can be utilized to diagnose milder forms of AKI potentially earlier in the course of the disease.

KW - Acute kidney injury

KW - Canine

KW - Chronic kidney disease

KW - Survival

KW - Urinary biomarker

UR - http://www.scopus.com/inward/record.url?scp=84887608719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887608719&partnerID=8YFLogxK

U2 - 10.1111/jvim.12180

DO - 10.1111/jvim.12180

M3 - Article

C2 - 24020513

AN - SCOPUS:84887608719

VL - 27

SP - 1362

EP - 1367

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

IS - 6

ER -