Evaluation of microbial flora in eyes with a Boston type 1 Keratoprosthesis

Samuel H. Lee, Mark J Mannis, Brett Shapiro, Jennifer Li, Christopher R Polage, Wayne Smith

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


PURPOSE:: To evaluate the microbial flora of eyes with a Boston Keratoprosthesis (K-Pro). METHODS:: A prospective study was performed for 17 eyes of 15 patients who underwent a K-Pro implantation between September 2005 and June 2011. Preoperative diagnoses included failed corneal grafts, limbal stem cell deficiency, chemical burns, and Stevens-Johnson Syndrome. The patients used topical antibiotics after their surgery including a fluoroquinolone, polymyxin-trimethoprim, vancomycin, or a combination of the 3. The conjunctiva in the study eye was swabbed and cultured. A separate culture was taken of the contralateral eye as well. If available, the bandage contact lens was removed, and half of it was placed in thioglycolate broth, and half in 5 mL of a sterile balanced salt solution. The contact lens in the balanced salt solution was sonicated using a QSonica Q125 sonicator (Newtown, CT) for 1 minute, at an amplitude of 20%. Ten microliters of fluid was subsequently cultured. RESULTS:: Of the patients who underwent the K-Pro surgery during that time period, 15 patients with 17 eyes were able to participate in the data collection. Nine of the 17 eyes implanted with the K-Pro (53%) had positive cultures. Two of the 13 (15%) of the control swabs exhibited bacterial growth. Eight percent (1/12) of the sonicated lenses were positive on culture, whereas 4/12 (33%) of the lenses placed in thioglycolate broth were positive for organisms. CONCLUSIONS:: Despite being on antibiotics, eyes implanted with the K-Pro were more likely to have a positive conjunctival culture in our cohort as compared with that of fellow eyes.

Original languageEnglish (US)
Pages (from-to)1537-1539
Number of pages3
Issue number12
StatePublished - Dec 2013


  • infectious endophthalmitis
  • K-Pro
  • K-Pro culture
  • keratoprosthesis
  • keratoprosthesis culture
  • sonication

ASJC Scopus subject areas

  • Ophthalmology


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