Evaluation of iodophor skin preparation techniques and factors influencing drainage from ventral midline incisions in horses

Larry D Galuppo, John Pascoe, Spencer S. Jang, Neil H. Willits, Sylvia L. Greenman

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Objective - To document natural bacterial flora on the ventral aspect of the equine abdomen, to compare 2 preparation techniques, and to identify potential risk factors that may contribute to incisional drainage. Design - Prospective study. Animals - 53 horses undergoing exploratory celiotomy. Procedure - Group-1 horses (n = 26) were prepared with povidone-iodine and alcohol. Group-2 horses (27) were prepared with a film-forming iodophor complex. Numbers of bacterial colony-forming units (CFU) were measured before and after surgical scrub, following skin closure, and after recovery from general anesthesia. Swab specimens to identify normal skin bacterial flora and potential pathogens were obtained by swabbing a 4 X 4-cm area. Variables that might affect incisional drainage were also investigated. Results - For both techniques, there was a significant reduction in bacterial numbers after skin preparation. Incisional drainage was observed in 14 (26%) horses (8 group-1 and 6 group-2 horses). Preexisting dermatitis, poor intraoperative drape adherence, high number of bacterial CFU obtained after recovery from anesthesia, and high number of CFU obtained from the surgery room environment were the main risk factors associated with subsequent incisional drainage. Bacillus spp, nonhemolytic Staphylococcus spp, Micrococcus spp, Corynebacterium spp, Streptomyces spp, other nonenteric genera, and non-hemolytic Streptococcus spp were the most common isolates obtained before surgical scrub. Conclusions and Clinical Relevance - Both skin preparation techniques were equally effective in reducing numbers of bacterial CFU by 99%, and a significant difference was not found in incisional drainage rate between groups. Protection of the wound during recovery from anesthesia and the immediate postoperative period may reduce incisional drainage after abdominal surgery in horses.

Original languageEnglish (US)
Pages (from-to)963-969
Number of pages7
JournalJournal of the American Veterinary Medical Association
Volume215
Issue number7
StatePublished - Oct 1 1999
Externally publishedYes

Fingerprint

Iodophors
skin (animal)
Horses
Drainage
drainage
horses
Skin
Stem Cells
anesthesia
shrublands
methodology
risk factors
Anesthesia
surgery
flora
Povidone-Iodine
Micrococcus
Corynebacterium
Streptomyces
Streptococcus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of iodophor skin preparation techniques and factors influencing drainage from ventral midline incisions in horses. / Galuppo, Larry D; Pascoe, John; Jang, Spencer S.; Willits, Neil H.; Greenman, Sylvia L.

In: Journal of the American Veterinary Medical Association, Vol. 215, No. 7, 01.10.1999, p. 963-969.

Research output: Contribution to journalArticle

@article{c250e2e303eb4ada848fe26f80b5f273,
title = "Evaluation of iodophor skin preparation techniques and factors influencing drainage from ventral midline incisions in horses",
abstract = "Objective - To document natural bacterial flora on the ventral aspect of the equine abdomen, to compare 2 preparation techniques, and to identify potential risk factors that may contribute to incisional drainage. Design - Prospective study. Animals - 53 horses undergoing exploratory celiotomy. Procedure - Group-1 horses (n = 26) were prepared with povidone-iodine and alcohol. Group-2 horses (27) were prepared with a film-forming iodophor complex. Numbers of bacterial colony-forming units (CFU) were measured before and after surgical scrub, following skin closure, and after recovery from general anesthesia. Swab specimens to identify normal skin bacterial flora and potential pathogens were obtained by swabbing a 4 X 4-cm area. Variables that might affect incisional drainage were also investigated. Results - For both techniques, there was a significant reduction in bacterial numbers after skin preparation. Incisional drainage was observed in 14 (26{\%}) horses (8 group-1 and 6 group-2 horses). Preexisting dermatitis, poor intraoperative drape adherence, high number of bacterial CFU obtained after recovery from anesthesia, and high number of CFU obtained from the surgery room environment were the main risk factors associated with subsequent incisional drainage. Bacillus spp, nonhemolytic Staphylococcus spp, Micrococcus spp, Corynebacterium spp, Streptomyces spp, other nonenteric genera, and non-hemolytic Streptococcus spp were the most common isolates obtained before surgical scrub. Conclusions and Clinical Relevance - Both skin preparation techniques were equally effective in reducing numbers of bacterial CFU by 99{\%}, and a significant difference was not found in incisional drainage rate between groups. Protection of the wound during recovery from anesthesia and the immediate postoperative period may reduce incisional drainage after abdominal surgery in horses.",
author = "Galuppo, {Larry D} and John Pascoe and Jang, {Spencer S.} and Willits, {Neil H.} and Greenman, {Sylvia L.}",
year = "1999",
month = "10",
day = "1",
language = "English (US)",
volume = "215",
pages = "963--969",
journal = "Journal of the American Veterinary Medical Association",
issn = "0003-1488",
publisher = "American Veterinary Medical Association",
number = "7",

}

TY - JOUR

T1 - Evaluation of iodophor skin preparation techniques and factors influencing drainage from ventral midline incisions in horses

AU - Galuppo, Larry D

AU - Pascoe, John

AU - Jang, Spencer S.

AU - Willits, Neil H.

AU - Greenman, Sylvia L.

PY - 1999/10/1

Y1 - 1999/10/1

N2 - Objective - To document natural bacterial flora on the ventral aspect of the equine abdomen, to compare 2 preparation techniques, and to identify potential risk factors that may contribute to incisional drainage. Design - Prospective study. Animals - 53 horses undergoing exploratory celiotomy. Procedure - Group-1 horses (n = 26) were prepared with povidone-iodine and alcohol. Group-2 horses (27) were prepared with a film-forming iodophor complex. Numbers of bacterial colony-forming units (CFU) were measured before and after surgical scrub, following skin closure, and after recovery from general anesthesia. Swab specimens to identify normal skin bacterial flora and potential pathogens were obtained by swabbing a 4 X 4-cm area. Variables that might affect incisional drainage were also investigated. Results - For both techniques, there was a significant reduction in bacterial numbers after skin preparation. Incisional drainage was observed in 14 (26%) horses (8 group-1 and 6 group-2 horses). Preexisting dermatitis, poor intraoperative drape adherence, high number of bacterial CFU obtained after recovery from anesthesia, and high number of CFU obtained from the surgery room environment were the main risk factors associated with subsequent incisional drainage. Bacillus spp, nonhemolytic Staphylococcus spp, Micrococcus spp, Corynebacterium spp, Streptomyces spp, other nonenteric genera, and non-hemolytic Streptococcus spp were the most common isolates obtained before surgical scrub. Conclusions and Clinical Relevance - Both skin preparation techniques were equally effective in reducing numbers of bacterial CFU by 99%, and a significant difference was not found in incisional drainage rate between groups. Protection of the wound during recovery from anesthesia and the immediate postoperative period may reduce incisional drainage after abdominal surgery in horses.

AB - Objective - To document natural bacterial flora on the ventral aspect of the equine abdomen, to compare 2 preparation techniques, and to identify potential risk factors that may contribute to incisional drainage. Design - Prospective study. Animals - 53 horses undergoing exploratory celiotomy. Procedure - Group-1 horses (n = 26) were prepared with povidone-iodine and alcohol. Group-2 horses (27) were prepared with a film-forming iodophor complex. Numbers of bacterial colony-forming units (CFU) were measured before and after surgical scrub, following skin closure, and after recovery from general anesthesia. Swab specimens to identify normal skin bacterial flora and potential pathogens were obtained by swabbing a 4 X 4-cm area. Variables that might affect incisional drainage were also investigated. Results - For both techniques, there was a significant reduction in bacterial numbers after skin preparation. Incisional drainage was observed in 14 (26%) horses (8 group-1 and 6 group-2 horses). Preexisting dermatitis, poor intraoperative drape adherence, high number of bacterial CFU obtained after recovery from anesthesia, and high number of CFU obtained from the surgery room environment were the main risk factors associated with subsequent incisional drainage. Bacillus spp, nonhemolytic Staphylococcus spp, Micrococcus spp, Corynebacterium spp, Streptomyces spp, other nonenteric genera, and non-hemolytic Streptococcus spp were the most common isolates obtained before surgical scrub. Conclusions and Clinical Relevance - Both skin preparation techniques were equally effective in reducing numbers of bacterial CFU by 99%, and a significant difference was not found in incisional drainage rate between groups. Protection of the wound during recovery from anesthesia and the immediate postoperative period may reduce incisional drainage after abdominal surgery in horses.

UR - http://www.scopus.com/inward/record.url?scp=0033208650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033208650&partnerID=8YFLogxK

M3 - Article

C2 - 10511861

AN - SCOPUS:0033208650

VL - 215

SP - 963

EP - 969

JO - Journal of the American Veterinary Medical Association

JF - Journal of the American Veterinary Medical Association

SN - 0003-1488

IS - 7

ER -