Evaluation of in vitro neuronal platforms as surrogates for in vivo whole brain systems

Anna M. Belle, Heather A. Enright, Ana Paula Sales, Kristen Kulp, Joanne Osburn, Edward A. Kuhn, Nicholas O Fischer, Elizabeth K. Wheeler

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Quantitatively benchmarking similarities and differences between the in vivo central nervous system and in vitro neuronal cultures can qualify discrepancies in functional responses and establish the utility of in vitro platforms. In this work, extracellular electrophysiology responses of cortical neurons in awake, freely-moving animals were compared to in vitro cultures of dissociated cortical neurons. After exposure to two well-characterized drugs, atropine and ketamine, a number of key points were observed: (1) significant differences in spontaneous firing activity for in vivo and in vitro systems, (2) similar response trends in single-unit spiking activity after exposure to atropine, and (3) greater sensitivity to the effects of ketamine in vitro. While in vitro cultures of dissociated cortical neurons may be appropriate for many types of pharmacological studies, we demonstrate that for some drugs, such as ketamine, this system may not fully capture the responses observed in vivo. Understanding the functionality associated with neuronal cultures will enhance the relevance of electrophysiology data sets and more accurately frame their conclusions. Comparing in vivo and in vitro rodent systems will provide the critical framework necessary for developing and interpreting in vitro systems using human cells that strive to more closely recapitulate human in vivo function and response.

Original languageEnglish (US)
Article number10820
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018
Externally publishedYes

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Brain
Ketamine
Electrophysiology
Atropine
Neurons
Benchmarking
In Vitro Techniques
Pharmaceutical Preparations
Rodentia
Central Nervous System
Pharmacology

ASJC Scopus subject areas

  • General

Cite this

Belle, A. M., Enright, H. A., Sales, A. P., Kulp, K., Osburn, J., Kuhn, E. A., ... Wheeler, E. K. (2018). Evaluation of in vitro neuronal platforms as surrogates for in vivo whole brain systems. Scientific Reports, 8(1), [10820]. https://doi.org/10.1038/s41598-018-28950-5

Evaluation of in vitro neuronal platforms as surrogates for in vivo whole brain systems. / Belle, Anna M.; Enright, Heather A.; Sales, Ana Paula; Kulp, Kristen; Osburn, Joanne; Kuhn, Edward A.; Fischer, Nicholas O; Wheeler, Elizabeth K.

In: Scientific Reports, Vol. 8, No. 1, 10820, 01.12.2018.

Research output: Contribution to journalArticle

Belle, AM, Enright, HA, Sales, AP, Kulp, K, Osburn, J, Kuhn, EA, Fischer, NO & Wheeler, EK 2018, 'Evaluation of in vitro neuronal platforms as surrogates for in vivo whole brain systems' Scientific Reports, vol. 8, no. 1, 10820. https://doi.org/10.1038/s41598-018-28950-5
Belle, Anna M. ; Enright, Heather A. ; Sales, Ana Paula ; Kulp, Kristen ; Osburn, Joanne ; Kuhn, Edward A. ; Fischer, Nicholas O ; Wheeler, Elizabeth K. / Evaluation of in vitro neuronal platforms as surrogates for in vivo whole brain systems. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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