Evaluation of glycoprotein Ib expression on feline platelets

Fern Tablin, Jocelyn D. Johnsrude, Naomi J. Walker

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective - To determine whether platelets obtained from cats expressed glycoprotein Ib (GPIb). Sample Population - Platelets obtained from 11 specific-pathogen-free cats. Procedure - Platelets were analyzed by use of immunofluorescence microscopy, flow cytometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western immunoblot analysis, and immunoprecipitation. Results - Immunofluorescence microscopy and flow cytometry revealed the protein on the surface of feline platelets. Biochemical studies (western immunoblot analysis and immunoprecipitation) revealed a 140-kd membrane glycoprotein. Additional biochemical studies revealed that feline GPIb was sensitive to proteolysis, because platelet cytoskeletons prepared with low concentrations of a calpain inhibitor (ie, leupeptin; 100 μg/ml) had substantial proteolysis, and there was an association of protein fragments with the actin cytoskeleton. Conclusions and Clinical Relevance - Analysis of these results indicate that feline platelets express a 140-kd membrane protein that is recognized by monoclonal antibodies developed against GPIb. Application of standardized ELISA to quantitate glycocalicin, the water-soluble fragment of GPIb, may provide important information on the production of microvesicles, increased platelet turnover, and abnormal proteolysis.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume62
Issue number2
StatePublished - Feb 2001

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Platelet Glycoprotein GPIb-IX Complex
Felidae
glycoproteins
Blood Platelets
cats
proteolysis
fluorescence microscopy
Proteolysis
flow cytometry
membrane glycoproteins
Fluorescence Microscopy
Immunoprecipitation
calpain
Flow Cytometry
Membrane Proteins
Cats
Western Blotting
cytoskeleton
microfilaments
membrane proteins

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of glycoprotein Ib expression on feline platelets. / Tablin, Fern; Johnsrude, Jocelyn D.; Walker, Naomi J.

In: American Journal of Veterinary Research, Vol. 62, No. 2, 02.2001, p. 195-201.

Research output: Contribution to journalArticle

Tablin, Fern ; Johnsrude, Jocelyn D. ; Walker, Naomi J. / Evaluation of glycoprotein Ib expression on feline platelets. In: American Journal of Veterinary Research. 2001 ; Vol. 62, No. 2. pp. 195-201.
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