Evaluation of Fast Technology Analysis (FTA) Cards as an improved method for specimen collection and shipment targeting viruses associated with Bovine Respiratory Disease Complex

Xiao Liang, Munashe Chigerwe, Sharon K. Hietala, Beate Crossley

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In order to improve the analytic quality of respiratory specimens collected from cattle for nucleic acid-based diagnosis, a study was undertaken to verify realtime PCR efficiency of specimens collected and stabilized on FTA Cards™, filter paper which is treated chemically. Nucleic acids collected using FTA Cards without the need for a cold-chain or special liquid media handling provided realtime PCR results consistent (96.8% agreement, kappa 0.923 [95% CI. = 0.89-0.96]) with the same specimens collected using traditional viral transport media and shipped on ice using the U.S. Department of Transportation mandated liquid handling requirements. Nucleic acid stabilization on FTA Cards was evaluated over a temperature range (-27. °C to +46. °C) for up to 14 days to mimic environmental conditions for diagnostic sample handling between collection and processing in a routine veterinary laboratory. No significant difference (P≥. 0.05) was observed in realtime PCR cycle threshold values over the temperature range and time storage conditions for Bovine Viral Diarrhea virus, Bovine Respiratory Syncytial virus, Bovine Coronavirus, and Bovine Herpesvirus I. The four viruses evaluated in the study are associated with Bovine Respiratory Disease Complex where improvements in ease and reliability of specimen collection and shipping would enhance the diagnostic quality of specimens collected in the field, and ultimately improve diagnostic efficiency.

Original languageEnglish (US)
Pages (from-to)69-72
Number of pages4
JournalJournal of Virological Methods
Volume202
DOIs
StatePublished - 2014

Fingerprint

Bovine Respiratory Disease Complex
Specimen Handling
Nucleic Acids
Viruses
Technology
Polymerase Chain Reaction
Bovine Coronavirus
Bovine respiratory syncytial virus
Bovine Viral Diarrhea Viruses
Refrigeration
Temperature
Herpesviridae
Ice

Keywords

  • Bovine respiratory disease complex
  • Diagnostic specimen
  • FTA Card

ASJC Scopus subject areas

  • Virology
  • Medicine(all)

Cite this

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title = "Evaluation of Fast Technology Analysis (FTA) Cards as an improved method for specimen collection and shipment targeting viruses associated with Bovine Respiratory Disease Complex",
abstract = "In order to improve the analytic quality of respiratory specimens collected from cattle for nucleic acid-based diagnosis, a study was undertaken to verify realtime PCR efficiency of specimens collected and stabilized on FTA Cards™, filter paper which is treated chemically. Nucleic acids collected using FTA Cards without the need for a cold-chain or special liquid media handling provided realtime PCR results consistent (96.8{\%} agreement, kappa 0.923 [95{\%} CI. = 0.89-0.96]) with the same specimens collected using traditional viral transport media and shipped on ice using the U.S. Department of Transportation mandated liquid handling requirements. Nucleic acid stabilization on FTA Cards was evaluated over a temperature range (-27. °C to +46. °C) for up to 14 days to mimic environmental conditions for diagnostic sample handling between collection and processing in a routine veterinary laboratory. No significant difference (P≥. 0.05) was observed in realtime PCR cycle threshold values over the temperature range and time storage conditions for Bovine Viral Diarrhea virus, Bovine Respiratory Syncytial virus, Bovine Coronavirus, and Bovine Herpesvirus I. The four viruses evaluated in the study are associated with Bovine Respiratory Disease Complex where improvements in ease and reliability of specimen collection and shipping would enhance the diagnostic quality of specimens collected in the field, and ultimately improve diagnostic efficiency.",
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author = "Xiao Liang and Munashe Chigerwe and Hietala, {Sharon K.} and Beate Crossley",
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AU - Chigerwe, Munashe

AU - Hietala, Sharon K.

AU - Crossley, Beate

PY - 2014

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