Evaluation of dried blood spots as an alternative sample matrix for equine antidoping analysis

Benjamin C. Moeller, Zicheng Yang

Research output: Contribution to journalArticlepeer-review

Abstract

Controlling the abuse of prohibited substances such as anabolic steroids, selective androgen receptor modulators, β-adrenoceptor agonists, and blood doping agents is of great interest to racing authorities. The use of dried blood spots (DBS) as an alternative sampling approach may be a feasible approach for controlling the use of these agents. To assess the feasibility of using DBS in equine blood, an 11-min liquid chromatography–mass spectrometry method was developed on a triple quadrupole mass spectrometer following extraction from Whatman 903 DBS cards. A total of 50 compounds across multiple compound classes were detectable with reproducible results. The stability was assessed with good results after almost 3 months of storage at ambient temperatures. These results suggest that the use of DBS may be a feasible alternative sampling approach in equine drug testing.

Original languageEnglish (US)
JournalDrug Testing and Analysis
DOIs
StateAccepted/In press - 2020

Keywords

  • antidoping
  • dried blood spots
  • horse
  • liquid chromatography–mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Pharmaceutical Science
  • Spectroscopy

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