Evaluation of cytotoxicity attributed to thimerosal on murine and human kidney cells

Eun Kee Park; Sally K. Mak; Dietmar Kültz; Bruce D. Hammock

doi:10.1080/15287390701551324

Evaluation of cytotoxicity attributed to thimerosal on murine and human kidney cells

Eun Kee Park, Sally K. Mak, Dietmar Kültz, Bruce D. Hammock

Entomology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 μM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 μM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 μM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.

Original language	English (US)
Pages (from-to)	2092-2095
Number of pages	4
Journal	Journal of Toxicology and Environmental Health - Part A: Current Issues
Volume	70
Issue number	24
DOIs	https://doi.org/10.1080/15287390701551324
State	Published - Jan 2007

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

Access to Document

10.1080/15287390701551324

Cite this

@article{8c518d7916ae43bfa066bae75ee4f22e,

title = "Evaluation of cytotoxicity attributed to thimerosal on murine and human kidney cells",

abstract = "Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 μM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 μM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 μM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.",

author = "Park, {Eun Kee} and Mak, {Sally K.} and Dietmar K{\"u}ltz and Hammock, {Bruce D.}",

year = "2007",

month = jan,

doi = "10.1080/15287390701551324",

language = "English (US)",

volume = "70",

pages = "2092--2095",

journal = "Journal of Toxicology and Environmental Health - Part A: Current Issues",

issn = "1528-7394",

publisher = "Taylor and Francis Ltd.",

number = "24",

}

TY - JOUR

T1 - Evaluation of cytotoxicity attributed to thimerosal on murine and human kidney cells

AU - Park, Eun Kee

AU - Mak, Sally K.

AU - Kültz, Dietmar

AU - Hammock, Bruce D.

PY - 2007/1

Y1 - 2007/1

N2 - Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 μM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 μM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 μM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.

AB - Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 μM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 μM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 μM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.

UR - http://www.scopus.com/inward/record.url?scp=36749063010&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36749063010&partnerID=8YFLogxK

U2 - 10.1080/15287390701551324

DO - 10.1080/15287390701551324

M3 - Article

C2 - 18049999

AN - SCOPUS:36749063010

VL - 70

SP - 2092

EP - 2095

JO - Journal of Toxicology and Environmental Health - Part A: Current Issues

JF - Journal of Toxicology and Environmental Health - Part A: Current Issues

SN - 1528-7394

IS - 24

ER -

Evaluation of cytotoxicity attributed to thimerosal on murine and human kidney cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this