Evaluation of cytotoxicity attributed to thimerosal on murine and human kidney cells

Eun Kee Park; Sally K. Mak; Dietmar Kültz; Bruce D. Hammock

doi:10.1080/15287390701551324

Evaluation of cytotoxicity attributed to thimerosal on murine and human kidney cells

Eun Kee Park, Sally K. Mak, Dietmar Kültz, Bruce D. Hammock

Entomology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 μM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 μM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 μM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.

Original language	English (US)
Pages (from-to)	2092-2095
Number of pages	4
Journal	Journal of Toxicology and Environmental Health - Part A: Current Issues
Volume	70
Issue number	24
DOIs	https://doi.org/10.1080/15287390701551324
State	Published - Jan 2007

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

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title = "Evaluation of cytotoxicity attributed to thimerosal on murine and human kidney cells",

abstract = "Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 μM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 μM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 μM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.",

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AU - Park, Eun Kee

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AU - Kültz, Dietmar

AU - Hammock, Bruce D.

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N2 - Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 μM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 μM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 μM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.

AB - Renal inner medullary collecting duct cells (mIMCD3) and human embryonic kidney cells (HEK293) were used for cytoscreening of thimerosal and mercury chloride (HgCl2). Thimerosal and HgCl2 acted in a concentration-dependent manner. In mIMCD3 cells the 24-h LC50 values for thimerosal, thiosalicylic acid, 2,2-dithiosalicylic acid, and 2-sulfobenzoic acid were 2.9, 2200, >1000, and >10,000 μM, respectively. The 24-h LC50 value for HgCl2 in mIMCD3 cells was 40 μM. In HEK293 cells, the 24-h LC50 value for thimerosal was 9.5 μM. These data demonstrate that the higher cytotoxicity produced by thimerosal on renal cells with respect to similar compounds without Hg may be related to this metal content. The present study also establishes mIMCD3 cells as a valuable model for evaluation of cytotoxicity of nephrotoxic compounds.

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