Evaluation of cross-reacting anti-human antibodies in the euthymic hairless guinea pig model (HGP) suggests that the HGP may be a model for the study of proliferative skin disease

Kathleen J. Smith, John S. Graham, Henry G. Skelton, Timothy O'Leary, Robert B. Moeller, Carlin V. Okerberg, Charles G. Hurst

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Animal models have an important role in cutaneous research. The guinea pig has proven to be a useful model in a wide spectrum of these cutaneous studies; however, its usefulness is often compromised by the need for depilation. A euthymic hairless guinea pig (HGP) model avoids the problems associated with depilation. Morphologically, as in human skin, these animals have a multi-cell-layer epidermis. Proliferation kinetic studies, as well as documentation of the degree of immunologic cross-reactivity between available antibodies to human cutaneous antigens, could extend the usefulness of this animal model. We performed a battery of anti-human antibodies on formalin fixed tissue, to a variety of antigens present within the skin and on inflammatory cells. These included CD3, UCHL-1, OPD4, L-26, KP-1, Factor XIIIa, S-100 protein, cytokeratin (AE1, AE3 and CK1), CAM 5.2, vimentin, CD 34, Factor VIII, fibronectin, SM actin, collagen IV, laminin, Bcl-2, p53, Ki-67, and PCNA. Cross-reacting antibodies included: CD3, S-100 protein, cytokeratin (AE1, AE3 and CK1), vimentin, Factor VIII, SM actin, collagen IV, p53, Ki-67, and PCNA. Although this battery of antibodies is limited, the markedly increased staining of Ki-67 and PCNA within keratinocytes in the epidermis as compared to normal human skin reflects a high proliferative rate. In addition, positive staining for p53, Ki-67, and PCNA may be useful in studying effects on cell cycle kinetics and apoptosis. Although inflammatory cells and stromal and basement membrane antibodies showed limited cross-reactivity, these as well as the other markers used in this study, may prove useful in the HGP model.

Original languageEnglish (US)
Pages (from-to)240-250
Number of pages11
JournalJournal of Dermatological Science
Volume14
Issue number3
DOIs
StatePublished - Mar 1997
Externally publishedYes

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Keywords

  • Hairless guinea pig
  • Ki-67
  • p53
  • PCNA
  • Proliferation kinetics

ASJC Scopus subject areas

  • Dermatology

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