Abstract
Objective - To evaluate the ability of commercially available Escherichia coli J5 bacterin to protect rabbits from experimental challenge with Pasteurella multocida. Animals - 40 P multocida-free New Zealand White rabbits. Procedures - Rabbits were assigned to 1 of 4 groups of 10 rabbits each. Three of the groups were inoculated SC with J5 bacterin at 8 weeks old. Inoculation was repeated 3 and 6 weeks later. The fourth group was not inoculated and served as controls. Groups 1, 2, and 3 were given 109, 108, and 107 colony forming units (CFU), respectively. Response was monitored by titer assessment, using an E coli J5 antigen capture ELISA. Five weeks after the last inoculation, all rabbits were challenged with P multocida and observed for an additional 5 weeks. Clinical, hematologic, serologic, culture, and necropsy data were collected. Results - Inoculation of rabbits with 109 CFU of E coli J5 bacterin-induced titers that were significantly greater than titers of rabbits vaccinated with 108 or 107 CFU or those in controls. The incidence of acute bacteremia was lower in rabbits with high titers. At necropsy, prevalence of lesions typical of P multocida was not significantly different among groups. Prevalence of histologic lesions was also not significantly different among groups. Conclusions and Clinical Relevance - Although the bacterin induced considerable antibody response and possibly reduced the rate of bacteremia, antibodies were not protective against long-term colonization or infection of the frontal sinuses or tympanic bullae by the challenge strain of P multocida. This bacterin in its currently available form is unlikely to aid in reducing the prevalence of pasteurellosis in rabbits.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 853-859 |
| Number of pages | 7 |
| Journal | American Journal of Veterinary Research |
| Volume | 60 |
| Issue number | 7 |
| State | Published - Jul 1999 |
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- veterinary(all)
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Evaluation of commercially available Escherichia coli J5 bacterin as protection against experimental challenge with Pasteurella multocida in rabbits. / Ruble, Randall P.; Cullor, James S; Brooks, Dale L.
In: American Journal of Veterinary Research, Vol. 60, No. 7, 07.1999, p. 853-859.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of commercially available Escherichia coli J5 bacterin as protection against experimental challenge with Pasteurella multocida in rabbits
AU - Ruble, Randall P.
AU - Cullor, James S
AU - Brooks, Dale L.
PY - 1999/7
Y1 - 1999/7
N2 - Objective - To evaluate the ability of commercially available Escherichia coli J5 bacterin to protect rabbits from experimental challenge with Pasteurella multocida. Animals - 40 P multocida-free New Zealand White rabbits. Procedures - Rabbits were assigned to 1 of 4 groups of 10 rabbits each. Three of the groups were inoculated SC with J5 bacterin at 8 weeks old. Inoculation was repeated 3 and 6 weeks later. The fourth group was not inoculated and served as controls. Groups 1, 2, and 3 were given 109, 108, and 107 colony forming units (CFU), respectively. Response was monitored by titer assessment, using an E coli J5 antigen capture ELISA. Five weeks after the last inoculation, all rabbits were challenged with P multocida and observed for an additional 5 weeks. Clinical, hematologic, serologic, culture, and necropsy data were collected. Results - Inoculation of rabbits with 109 CFU of E coli J5 bacterin-induced titers that were significantly greater than titers of rabbits vaccinated with 108 or 107 CFU or those in controls. The incidence of acute bacteremia was lower in rabbits with high titers. At necropsy, prevalence of lesions typical of P multocida was not significantly different among groups. Prevalence of histologic lesions was also not significantly different among groups. Conclusions and Clinical Relevance - Although the bacterin induced considerable antibody response and possibly reduced the rate of bacteremia, antibodies were not protective against long-term colonization or infection of the frontal sinuses or tympanic bullae by the challenge strain of P multocida. This bacterin in its currently available form is unlikely to aid in reducing the prevalence of pasteurellosis in rabbits.
AB - Objective - To evaluate the ability of commercially available Escherichia coli J5 bacterin to protect rabbits from experimental challenge with Pasteurella multocida. Animals - 40 P multocida-free New Zealand White rabbits. Procedures - Rabbits were assigned to 1 of 4 groups of 10 rabbits each. Three of the groups were inoculated SC with J5 bacterin at 8 weeks old. Inoculation was repeated 3 and 6 weeks later. The fourth group was not inoculated and served as controls. Groups 1, 2, and 3 were given 109, 108, and 107 colony forming units (CFU), respectively. Response was monitored by titer assessment, using an E coli J5 antigen capture ELISA. Five weeks after the last inoculation, all rabbits were challenged with P multocida and observed for an additional 5 weeks. Clinical, hematologic, serologic, culture, and necropsy data were collected. Results - Inoculation of rabbits with 109 CFU of E coli J5 bacterin-induced titers that were significantly greater than titers of rabbits vaccinated with 108 or 107 CFU or those in controls. The incidence of acute bacteremia was lower in rabbits with high titers. At necropsy, prevalence of lesions typical of P multocida was not significantly different among groups. Prevalence of histologic lesions was also not significantly different among groups. Conclusions and Clinical Relevance - Although the bacterin induced considerable antibody response and possibly reduced the rate of bacteremia, antibodies were not protective against long-term colonization or infection of the frontal sinuses or tympanic bullae by the challenge strain of P multocida. This bacterin in its currently available form is unlikely to aid in reducing the prevalence of pasteurellosis in rabbits.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033159625&partnerID=8YFLogxK
M3 - Article
C2 - 10407479
AN - SCOPUS:0033159625
VL - 60
SP - 853
EP - 859
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 7
ER -