Evaluation of Coccidioides antigen detection in dogs with coccidioidomycosis

Emily J. Kirsch, Russell T. Greene, Annalisa Prahl, Stanley I. Rubin, Jane E Sykes, Michelle M. Durkin, Lawrence J. Wheat

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Antigen detection has been reported to be a promising method for rapid diagnosis of coccidioidomycosis in humans. Coccidioides antigen detection has not been previously reported in dogs with coccidioidomycosis and was evaluated in 60 cases diagnosed based on detection of anti-Coccidioides antibodies at titers of 1:16 or more in serum. Controls included dogs with presumed histoplasmosis or blastomycosis, other fungal infections, or nonfungal diseases and healthy dogs. Urine and serum specimens were tested using an enzyme immunoassay for Coccidioides galactomannan antigen. Antibody testing was performed at commercial veterinary reference laboratories. Antigen was detected in urine or serum of 12 of 60 (20.0%), urine only in 2 of 57 (3.5%), and serum only in 11 of 58 (19.0%) dogs with coccidioidomycosis. Antigen was detected in the urine of 3 of 43 (7.0%) and serum of 1 of 37 (2.7%) dogs with histoplasmosis or blastomycosis but not in 13 dogs with other fungal infections (serum, 9; urine, 13), 41 dogs with nonfungal diseases (urine, 41; serum, 18), or healthy dogs (serum, 21; urine, 21). Detection of antigen was an insensitive method for diagnosis of coccidioidomycosis in dogs in which the diagnosis was based primarily upon detection of antibodies at titers of 1:16 or higher, and the highest sensitivity was in serum.

Original languageEnglish (US)
Pages (from-to)343-345
Number of pages3
JournalClinical and Vaccine Immunology
Volume19
Issue number3
DOIs
StatePublished - Mar 2012

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

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    Kirsch, E. J., Greene, R. T., Prahl, A., Rubin, S. I., Sykes, J. E., Durkin, M. M., & Wheat, L. J. (2012). Evaluation of Coccidioides antigen detection in dogs with coccidioidomycosis. Clinical and Vaccine Immunology, 19(3), 343-345. https://doi.org/10.1128/CVI.05631-11