Evaluation of Clostridium difficile fecal load and limit of detection during a prospective comparison of two molecular tests, the illumigene C. difficile and xpert C. difficile/epi tests

Clare E. Gyorke, Susan Wang, Jhansi L. Leslie, Stuart H Cohen, Jay V Solnick, Christopher R Polage

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In a large prospective comparison, the illumigene test detected Clostridium difficile in 98% of toxin-positive and 58% of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of detection. The significance of low-level C. difficile detection needs investigation.

Original languageEnglish (US)
Pages (from-to)278-280
Number of pages3
JournalJournal of Clinical Microbiology
Volume51
Issue number1
DOIs
StatePublished - Jan 2013

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Clostridium difficile
Limit of Detection

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

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abstract = "In a large prospective comparison, the illumigene test detected Clostridium difficile in 98{\%} of toxin-positive and 58{\%} of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of detection. The significance of low-level C. difficile detection needs investigation.",
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AU - Wang, Susan

AU - Leslie, Jhansi L.

AU - Cohen, Stuart H

AU - Solnick, Jay V

AU - Polage, Christopher R

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AB - In a large prospective comparison, the illumigene test detected Clostridium difficile in 98% of toxin-positive and 58% of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of detection. The significance of low-level C. difficile detection needs investigation.

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