Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients

Patricia I. Oteiza, Osvaldo D. Uchitel, Fernando Carrasquedo, Alberto L. Dubrovski, Juan C. Roma, Cesar G. Fraga

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Several parameters indicators of oxidative stress were evaluated in blood from individuals with the sporadic form of amiotrophic lateral sclerosis (SALS) and compared to healthy controls. Plasma levels of 2- thiobarbituric-reactive substances (TBARS), products of lipid peroxidation, were significantly higher (p < 0.03) in the SALS patients compared to controls. The concentration of plasma antioxidants (α-tocopherol, β- carotene, ubiquinol-10 and glutathione) and the activity of red blood cell CuZn superoxide dismutase were not significantly different between the groups. The ratio TBARS/α-tocopherol was 47% higher in the SALS individuals than in controls. Protein thiols and protein-associated carbonyls in red blood cell membranes and supernates were similar for both groups. A positive correlation (r 2 = 0.91) was found between the concentration of protein- associated carbonyls in red blood cells and the onset of clinical symptoms. These findings are in agreement with several reports showing higher levels of oxidative damage to cell components in ALS.

Original languageEnglish (US)
Pages (from-to)535-539
Number of pages5
JournalNeurochemical Research
Volume22
Issue number4
StatePublished - 1997
Externally publishedYes

Fingerprint

Motor Neuron Disease
Blood
Tocopherols
Antioxidants
Erythrocytes
Lipids
Oxidation
Proteins
Cells
Cellular Structures
Carotenoids
Plasmas
Sulfhydryl Compounds
Lipid Peroxidation
Superoxide Dismutase
Oxidative stress
Glutathione
Oxidative Stress
Cell membranes
Cell Membrane

Keywords

  • ALS
  • Antioxidants
  • neurodegenerative
  • oxygen species
  • superoxide dismutase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Oteiza, P. I., Uchitel, O. D., Carrasquedo, F., Dubrovski, A. L., Roma, J. C., & Fraga, C. G. (1997). Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients. Neurochemical Research, 22(4), 535-539.

Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients. / Oteiza, Patricia I.; Uchitel, Osvaldo D.; Carrasquedo, Fernando; Dubrovski, Alberto L.; Roma, Juan C.; Fraga, Cesar G.

In: Neurochemical Research, Vol. 22, No. 4, 1997, p. 535-539.

Research output: Contribution to journalArticle

Oteiza, PI, Uchitel, OD, Carrasquedo, F, Dubrovski, AL, Roma, JC & Fraga, CG 1997, 'Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients', Neurochemical Research, vol. 22, no. 4, pp. 535-539.
Oteiza, Patricia I. ; Uchitel, Osvaldo D. ; Carrasquedo, Fernando ; Dubrovski, Alberto L. ; Roma, Juan C. ; Fraga, Cesar G. / Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients. In: Neurochemical Research. 1997 ; Vol. 22, No. 4. pp. 535-539.
@article{76b2a8aabc9144a58d96d3c6f6077d16,
title = "Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients",
abstract = "Several parameters indicators of oxidative stress were evaluated in blood from individuals with the sporadic form of amiotrophic lateral sclerosis (SALS) and compared to healthy controls. Plasma levels of 2- thiobarbituric-reactive substances (TBARS), products of lipid peroxidation, were significantly higher (p < 0.03) in the SALS patients compared to controls. The concentration of plasma antioxidants (α-tocopherol, β- carotene, ubiquinol-10 and glutathione) and the activity of red blood cell CuZn superoxide dismutase were not significantly different between the groups. The ratio TBARS/α-tocopherol was 47{\%} higher in the SALS individuals than in controls. Protein thiols and protein-associated carbonyls in red blood cell membranes and supernates were similar for both groups. A positive correlation (r 2 = 0.91) was found between the concentration of protein- associated carbonyls in red blood cells and the onset of clinical symptoms. These findings are in agreement with several reports showing higher levels of oxidative damage to cell components in ALS.",
keywords = "ALS, Antioxidants, neurodegenerative, oxygen species, superoxide dismutase",
author = "Oteiza, {Patricia I.} and Uchitel, {Osvaldo D.} and Fernando Carrasquedo and Dubrovski, {Alberto L.} and Roma, {Juan C.} and Fraga, {Cesar G.}",
year = "1997",
language = "English (US)",
volume = "22",
pages = "535--539",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients

AU - Oteiza, Patricia I.

AU - Uchitel, Osvaldo D.

AU - Carrasquedo, Fernando

AU - Dubrovski, Alberto L.

AU - Roma, Juan C.

AU - Fraga, Cesar G.

PY - 1997

Y1 - 1997

N2 - Several parameters indicators of oxidative stress were evaluated in blood from individuals with the sporadic form of amiotrophic lateral sclerosis (SALS) and compared to healthy controls. Plasma levels of 2- thiobarbituric-reactive substances (TBARS), products of lipid peroxidation, were significantly higher (p < 0.03) in the SALS patients compared to controls. The concentration of plasma antioxidants (α-tocopherol, β- carotene, ubiquinol-10 and glutathione) and the activity of red blood cell CuZn superoxide dismutase were not significantly different between the groups. The ratio TBARS/α-tocopherol was 47% higher in the SALS individuals than in controls. Protein thiols and protein-associated carbonyls in red blood cell membranes and supernates were similar for both groups. A positive correlation (r 2 = 0.91) was found between the concentration of protein- associated carbonyls in red blood cells and the onset of clinical symptoms. These findings are in agreement with several reports showing higher levels of oxidative damage to cell components in ALS.

AB - Several parameters indicators of oxidative stress were evaluated in blood from individuals with the sporadic form of amiotrophic lateral sclerosis (SALS) and compared to healthy controls. Plasma levels of 2- thiobarbituric-reactive substances (TBARS), products of lipid peroxidation, were significantly higher (p < 0.03) in the SALS patients compared to controls. The concentration of plasma antioxidants (α-tocopherol, β- carotene, ubiquinol-10 and glutathione) and the activity of red blood cell CuZn superoxide dismutase were not significantly different between the groups. The ratio TBARS/α-tocopherol was 47% higher in the SALS individuals than in controls. Protein thiols and protein-associated carbonyls in red blood cell membranes and supernates were similar for both groups. A positive correlation (r 2 = 0.91) was found between the concentration of protein- associated carbonyls in red blood cells and the onset of clinical symptoms. These findings are in agreement with several reports showing higher levels of oxidative damage to cell components in ALS.

KW - ALS

KW - Antioxidants

KW - neurodegenerative

KW - oxygen species

KW - superoxide dismutase

UR - http://www.scopus.com/inward/record.url?scp=0030968429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030968429&partnerID=8YFLogxK

M3 - Article

C2 - 9130267

AN - SCOPUS:0030968429

VL - 22

SP - 535

EP - 539

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 4

ER -