Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients

Patricia I. Oteiza, Osvaldo D. Uchitel, Fernando Carrasquedo, Alberto L. Dubrovski, Juan C. Roma, Cesar G. Fraga

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

Several parameters indicators of oxidative stress were evaluated in blood from individuals with the sporadic form of amiotrophic lateral sclerosis (SALS) and compared to healthy controls. Plasma levels of 2- thiobarbituric-reactive substances (TBARS), products of lipid peroxidation, were significantly higher (p < 0.03) in the SALS patients compared to controls. The concentration of plasma antioxidants (α-tocopherol, β- carotene, ubiquinol-10 and glutathione) and the activity of red blood cell CuZn superoxide dismutase were not significantly different between the groups. The ratio TBARS/α-tocopherol was 47% higher in the SALS individuals than in controls. Protein thiols and protein-associated carbonyls in red blood cell membranes and supernates were similar for both groups. A positive correlation (r 2 = 0.91) was found between the concentration of protein- associated carbonyls in red blood cells and the onset of clinical symptoms. These findings are in agreement with several reports showing higher levels of oxidative damage to cell components in ALS.

Original languageEnglish (US)
Pages (from-to)535-539
Number of pages5
JournalNeurochemical Research
Volume22
Issue number4
StatePublished - 1997
Externally publishedYes

Keywords

  • ALS
  • Antioxidants
  • neurodegenerative
  • oxygen species
  • superoxide dismutase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Fingerprint Dive into the research topics of 'Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients'. Together they form a unique fingerprint.

  • Cite this

    Oteiza, P. I., Uchitel, O. D., Carrasquedo, F., Dubrovski, A. L., Roma, J. C., & Fraga, C. G. (1997). Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amiotrophic lateral sclerosis patients. Neurochemical Research, 22(4), 535-539.