Evaluation of an indirect fluorescent antibody test (IFAT) for demonstration of antibodies to Toxoplasma gondii in the sea otter (Enhydra lutris)

M. A. Miller, Ian Gardner, A. Packham, Jonna A Mazet, K. D. Hanni, D. Jessup, J. Estes, R. Jameson, E. Dodd, B. C. Barr, Linda J Lowenstine, F. M. Gulland, Patricia A Conrad

Research output: Contribution to journalArticle

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Abstract

An indirect fluorescent antibody test (IFAT) for detection of Toxoplasma gondii infection was validated using serum from 77 necropsied southern sea otters (Enhydra lutris nereis) whose T. gondii infection status was determined through immunohistochemistry and parasite isolation in cell culture. Twenty-eight otters (36%) were positive for T. gondii by immunohisto-chemistry or parasite isolation or both, whereas 49 (64%) were negative by both tests. At a cutoff of 1:320, combined values for IFAT sensitivity and specificity were maximized at 96.4 and 67.3%, respectively. The area under the receiver-operating characteristic curve for the IFAT was 0.84. A titer of 1:320 was used as cutoff when screening serum collected from live-sampled sea otters from California (n = 80), Washington (n = 21), and Alaska (n = 65) for T. gondii infection. Thirty-six percent (29 out of 80) of California sea otters (E. lutris nereis) and 38% (8 out of 21) of Washington sea otters (E. lutris kenyoni) were seropositive for T. gondii, compared with 0% (0 out of 65) of Alaskan sea otters (E. lutris kenyoni).

Original languageEnglish (US)
Pages (from-to)594-599
Number of pages6
JournalJournal of Parasitology
Volume88
Issue number3
StatePublished - 2002

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Otters
Enhydra lutris
Toxoplasma
Toxoplasma gondii
antibody
antibodies
Antibodies
testing
serum
Toxoplasmosis
parasite
infection
parasites
Parasites
immunohistochemistry
evaluation
test
sea
chemistry
cell culture

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

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Evaluation of an indirect fluorescent antibody test (IFAT) for demonstration of antibodies to Toxoplasma gondii in the sea otter (Enhydra lutris). / Miller, M. A.; Gardner, Ian; Packham, A.; Mazet, Jonna A; Hanni, K. D.; Jessup, D.; Estes, J.; Jameson, R.; Dodd, E.; Barr, B. C.; Lowenstine, Linda J; Gulland, F. M.; Conrad, Patricia A.

In: Journal of Parasitology, Vol. 88, No. 3, 2002, p. 594-599.

Research output: Contribution to journalArticle

Miller, M. A. ; Gardner, Ian ; Packham, A. ; Mazet, Jonna A ; Hanni, K. D. ; Jessup, D. ; Estes, J. ; Jameson, R. ; Dodd, E. ; Barr, B. C. ; Lowenstine, Linda J ; Gulland, F. M. ; Conrad, Patricia A. / Evaluation of an indirect fluorescent antibody test (IFAT) for demonstration of antibodies to Toxoplasma gondii in the sea otter (Enhydra lutris). In: Journal of Parasitology. 2002 ; Vol. 88, No. 3. pp. 594-599.
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abstract = "An indirect fluorescent antibody test (IFAT) for detection of Toxoplasma gondii infection was validated using serum from 77 necropsied southern sea otters (Enhydra lutris nereis) whose T. gondii infection status was determined through immunohistochemistry and parasite isolation in cell culture. Twenty-eight otters (36{\%}) were positive for T. gondii by immunohisto-chemistry or parasite isolation or both, whereas 49 (64{\%}) were negative by both tests. At a cutoff of 1:320, combined values for IFAT sensitivity and specificity were maximized at 96.4 and 67.3{\%}, respectively. The area under the receiver-operating characteristic curve for the IFAT was 0.84. A titer of 1:320 was used as cutoff when screening serum collected from live-sampled sea otters from California (n = 80), Washington (n = 21), and Alaska (n = 65) for T. gondii infection. Thirty-six percent (29 out of 80) of California sea otters (E. lutris nereis) and 38{\%} (8 out of 21) of Washington sea otters (E. lutris kenyoni) were seropositive for T. gondii, compared with 0{\%} (0 out of 65) of Alaskan sea otters (E. lutris kenyoni).",
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