Evaluation of an agar gel immunodiffusion test to detect infection of cattle with bluetongue viruses in Queensland, Australia

M. P. Ward, Ian Gardner, M. Flanagan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An agar gel immunodiffusion (AGID) test to detect group-specific antibodies to infection of cattle by bluetongue viruses was evaluated using field collected sera in Queensland, Australia. The AGID test was compared to the serum neutralisation (SN) test used to detect serotype-specific bluetongue virus antibodies. The AGID test was found to be highly sensitive (95% confidence interval, 80.7-100%) but to have moderate specificity (95% confidence interval, 59.3-79.6%), relative to the SN test. The correlation between AGID and SN test results was 0.41 (P < 0.001). Using likelihood ratio estimates the chance (posttest probability) of an AGID test positive serum sample being positive (titre ≥ 1:20) in the SN test was estimated to be 87% and 90% for 2+ and 3+ AGID test reactions, respectively. A serum sample was estimated to have a probability of 40% of being positive in the SN test if it gave a 1+ reaction in the AGID test. Results suggest that the AGID test should ideally be used as a screening test in serological surveys and surveillance programs. If the test is to be used for identification of individually infected cattle, a cutoff point of ≥ 2+ should be employed.

Original languageEnglish (US)
Pages (from-to)27-34
Number of pages8
JournalVeterinary Microbiology
Volume45
Issue number1
DOIs
StatePublished - Jan 1 1995

Fingerprint

Bluetongue virus
immunodiffusion tests
Queensland
Immunodiffusion
Agar
Neutralization Tests
agar
Gels
gels
neutralization tests
cattle
Infection
Serum
infection
confidence interval
Confidence Intervals
antibodies
Antibodies
Serologic Tests
serological surveys

Keywords

  • Australia
  • Bluetongue virus
  • Cattle
  • Serology

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Evaluation of an agar gel immunodiffusion test to detect infection of cattle with bluetongue viruses in Queensland, Australia. / Ward, M. P.; Gardner, Ian; Flanagan, M.

In: Veterinary Microbiology, Vol. 45, No. 1, 01.01.1995, p. 27-34.

Research output: Contribution to journalArticle

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