Evaluation of age-related effects on the antiviral activity of interferon and induction of 2-5A synthetase in testicular cell cultures derived from swine of various ages

B. T. Bosworth, Nigel J Maclachlan

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The antiviral activity of recombinant DNA-derived bovine alpha1-1 interferon on an established swine testicular cell line and primary testicular cell cultures derived from swine of various ages (2 days, 3 weeks, and 5 weeks) was determined. Bovine interferon induced a dose-dependent increase in 2-5A synthetase in testicular cells, regardless of the source of the cells. Furthermore, interferon inhibited replication of vesicular stomatitis virus to an equivalent extent in all testicular cell cultures. The results indicate that 2-5A synthetase is a reliable marker of interferon activity in swine testicular cell cultures and that the induction of 2-5A synthetase and antiviral effects of recombinant bovine interferon in primary testicular cell cultures are not dependent on the age of the donor animal.

Original languageEnglish (US)
Pages (from-to)723-725
Number of pages3
JournalAmerican Journal of Veterinary Research
Volume51
Issue number5
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • veterinary(all)

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