Evaluation of a stereotactic frame for repositioning of the rat brain in serial positron emission tomography imaging studies

Daniel J. Rubins, A. Ken Meadors, Simon Yee, William P. Melega, Simon R Cherry

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

For serial imaging studies of the rat brain with positron emission tomography (PET), reproducible positioning of the head can facilitate spatial alignment of images and quantitative analysis. To achieve this aim, we constructed a plastic head frame and tested the positioning reproducibility on a high-resolution small-animal PET scanner, microPET. Two sets of ear bars, with tapers of either 18° (sharp) or 45° (blunt), were evaluated for their relative precision in securing the animal to the frame. For sequential positioning of an animal, average distances from the mean position of 0.51mm (SD 0.41mm) and 0.91mm (SD 0.48mm) were measured with the sharp and blunt ear bars, respectively. These results show that a rat brain can be reproducibly positioned using the frame, with a variation of position less than the spatial resolution of modern animal PET scanners. Brain regions of interest defined on one scan and copied across subsequent scans of a frame-repositioned animal resulted in an average coefficient of variation of 5.4% (SD 2.7%) using the sharp ear bars and 6.8% (SD 2.5%) using the blunt ear bars. This methodology has the potential to improve quantitative assessment for serial PET studies.

Original languageEnglish (US)
Pages (from-to)63-70
Number of pages8
JournalJournal of Neuroscience Methods
Volume107
Issue number1-2
DOIs
StatePublished - May 30 2001

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Positron-Emission Tomography
Ear
Brain
Plastics
Head

Keywords

  • Image data analysis
  • Imaging instrumentation
  • In vivo imaging of small animals
  • Neuroimaging
  • Positron emission tomography
  • Rat model of disease
  • Stereotactic head holder

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Evaluation of a stereotactic frame for repositioning of the rat brain in serial positron emission tomography imaging studies. / Rubins, Daniel J.; Meadors, A. Ken; Yee, Simon; Melega, William P.; Cherry, Simon R.

In: Journal of Neuroscience Methods, Vol. 107, No. 1-2, 30.05.2001, p. 63-70.

Research output: Contribution to journalArticle

Rubins, Daniel J. ; Meadors, A. Ken ; Yee, Simon ; Melega, William P. ; Cherry, Simon R. / Evaluation of a stereotactic frame for repositioning of the rat brain in serial positron emission tomography imaging studies. In: Journal of Neuroscience Methods. 2001 ; Vol. 107, No. 1-2. pp. 63-70.
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