Abstract
We evaluated the performance of a direct Liquid N-geneous HDL-C assay (N-HDL; Genzyme Diagnostics, Cambridge, Mass) and compared it with a Centers for Disease Control and Prevention (CDC) modified reference procedure (M- REF) and phosphotungstic acid (PTA) precipitation method in patients with normotriglyceridemia (triglyceride level, <400 mg/dL) and hypertriglyceridemia (triglyceride level, ≤400 mg/dL). Excellent intra- assay and interassay coefficients of variation were obtained (<2.0%) using the N-HDL assay. The N-HDL and PTA assays correlated well with M-REF in normotriglyceridemic samples. In hypertriglyceridemic samples, however, the N-HDL method exhibited better correlation with M-REF than the PTA assay. In addition, compared with M-REF, the mean absolute percentage bias of N-HDL was lower than the PTA assay in normotriglyceridemic (4.9% vs 5.8%) and hypertriglyceridemic (5.4% vs 12.9%) samples. Hemolysis, ascorbic acid, and bilirubin did not interfere with the N-HDL assay. On the basis of these findings, the N-HDL assay compares favorably with the modified CDC reference method and seems superior to the PTA assay. It also has the advantage of being suited for complete automation and, thus, would prove useful in large clinical laboratories.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 495-502 |
| Number of pages | 8 |
| Journal | American Journal of Clinical Pathology |
| Volume | 110 |
| Issue number | 4 |
| State | Published - Oct 1998 |
| Externally published | Yes |
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Keywords
- Coronary artery disease
- High-density lipoprotein cholesterol
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Evaluation of a rapid homogeneous method for direct measurement of high- density lipoprotein cholesterol. / Hubbard, R. Scott; Hirany, Shaina V.; Devaraj, Sridevi; Martin, Lani; Parupia, Joseph; Jialal, Ishwarlal.
In: American Journal of Clinical Pathology, Vol. 110, No. 4, 10.1998, p. 495-502.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of a rapid homogeneous method for direct measurement of high- density lipoprotein cholesterol
AU - Hubbard, R. Scott
AU - Hirany, Shaina V.
AU - Devaraj, Sridevi
AU - Martin, Lani
AU - Parupia, Joseph
AU - Jialal, Ishwarlal
PY - 1998/10
Y1 - 1998/10
N2 - We evaluated the performance of a direct Liquid N-geneous HDL-C assay (N-HDL; Genzyme Diagnostics, Cambridge, Mass) and compared it with a Centers for Disease Control and Prevention (CDC) modified reference procedure (M- REF) and phosphotungstic acid (PTA) precipitation method in patients with normotriglyceridemia (triglyceride level, <400 mg/dL) and hypertriglyceridemia (triglyceride level, ≤400 mg/dL). Excellent intra- assay and interassay coefficients of variation were obtained (<2.0%) using the N-HDL assay. The N-HDL and PTA assays correlated well with M-REF in normotriglyceridemic samples. In hypertriglyceridemic samples, however, the N-HDL method exhibited better correlation with M-REF than the PTA assay. In addition, compared with M-REF, the mean absolute percentage bias of N-HDL was lower than the PTA assay in normotriglyceridemic (4.9% vs 5.8%) and hypertriglyceridemic (5.4% vs 12.9%) samples. Hemolysis, ascorbic acid, and bilirubin did not interfere with the N-HDL assay. On the basis of these findings, the N-HDL assay compares favorably with the modified CDC reference method and seems superior to the PTA assay. It also has the advantage of being suited for complete automation and, thus, would prove useful in large clinical laboratories.
AB - We evaluated the performance of a direct Liquid N-geneous HDL-C assay (N-HDL; Genzyme Diagnostics, Cambridge, Mass) and compared it with a Centers for Disease Control and Prevention (CDC) modified reference procedure (M- REF) and phosphotungstic acid (PTA) precipitation method in patients with normotriglyceridemia (triglyceride level, <400 mg/dL) and hypertriglyceridemia (triglyceride level, ≤400 mg/dL). Excellent intra- assay and interassay coefficients of variation were obtained (<2.0%) using the N-HDL assay. The N-HDL and PTA assays correlated well with M-REF in normotriglyceridemic samples. In hypertriglyceridemic samples, however, the N-HDL method exhibited better correlation with M-REF than the PTA assay. In addition, compared with M-REF, the mean absolute percentage bias of N-HDL was lower than the PTA assay in normotriglyceridemic (4.9% vs 5.8%) and hypertriglyceridemic (5.4% vs 12.9%) samples. Hemolysis, ascorbic acid, and bilirubin did not interfere with the N-HDL assay. On the basis of these findings, the N-HDL assay compares favorably with the modified CDC reference method and seems superior to the PTA assay. It also has the advantage of being suited for complete automation and, thus, would prove useful in large clinical laboratories.
KW - Coronary artery disease
KW - High-density lipoprotein cholesterol
UR - http://www.scopus.com/inward/record.url?scp=0031712430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031712430&partnerID=8YFLogxK
M3 - Article
C2 - 9763036
AN - SCOPUS:0031712430
VL - 110
SP - 495
EP - 502
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -