Evaluation of a range of doses of ultraviolet irradiation to inactivate waterborne actinospore stages of Myxobolus cerebralis

Ronald Hedrick, B. Petri, T. S. McDowell, K. Mukkatira, L. J. Sealey

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The ability of a range of doses of ultraviolet irradiation (UV) to inactivate the waterborne actinospore or triactinomyxon stages (TAMs) of Myxobolus cerebralis was evaluated by infectivity for juvenile rainbow trout Oncorhynchus mykiss. TAMs were UV-irradiated using a low pressure mercury vapour lamp collimated beam apparatus. All doses 40, 80, 120 and 160 mJ cm -2 were found to completely inactivate the TAMs as demonstrated by the absence of microscopic lesions, myxospores and parasite DNA detected by quantitative PCR (qPCR) among rainbow trout 5 mo post-exposure. In contrast, rainbow trout receiving the same concentrations of untreated TAMs (1000 fish-1) developed clinical signs of whirling disease at 2 mo post-exposure and had severe microscopic lesions, high myxospore counts and high qPCR values when examined at 5 mo following exposure to the parasite.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalDiseases of Aquatic Organisms
Volume74
Issue number2
DOIs
StatePublished - Feb 28 2007

Fingerprint

Myxobolus cerebralis
rainbow
Oncorhynchus mykiss
irradiation
lesion
parasite
dosage
lesions (animal)
quantitative polymerase chain reaction
infectivity
whirling disease
parasites
low pressure
vapors
mercury
DNA
pathogenicity
fish
dose
evaluation

Keywords

  • Disease control
  • Fish hatchery
  • Ultraviolet irradiation
  • Whirling disease

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Evaluation of a range of doses of ultraviolet irradiation to inactivate waterborne actinospore stages of Myxobolus cerebralis. / Hedrick, Ronald; Petri, B.; McDowell, T. S.; Mukkatira, K.; Sealey, L. J.

In: Diseases of Aquatic Organisms, Vol. 74, No. 2, 28.02.2007, p. 113-118.

Research output: Contribution to journalArticle

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