Abstract
Anopheles mosquitoes are routinely identified using morphological characters of the female that often lead to misidentification due to interspecies similarity and intraspecies variability. The aim of this work was to evaluate the applicability of a previously developed PCR-RFLP-ITS2 assay for accurate discrimination of anophelines in twelve localities spanning three Colombian malaria epidemiological regions: Atlantic Coast, Pacific Coast, and Uraba-Bajo Cauca-Alto Sinu region. The evaluation of the stability of the PCR-RFLP patterns is required since variability of the ITS2 has been documented and may produce discrepancies in the patterns previously reported. The assay was used to evaluate species assignation of 939 mosquitoes identified by morphology. Strong agreement between the morphological and molecular identification was found for species Anopheles albimanus, Anopheles aquasalis, Anopheles darlingi and Anopheles triannulatus s.l. (p≥0.05, kappa. =1). However, disagreement was found for species Anopheles nuneztovari s.l., Anopheles neomaculipalpus, Anopheles apicimacula and Anopheles punctimacula (p≤0.05; kappa ranging from 0.33 to 0.80). The ITS2-PCR-RFLP assay proved valuable for discriminating anopheline species of northern and western Colombia, especially those with overlapping morphology in the Oswaldoi Group.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 128-135 |
| Number of pages | 8 |
| Journal | Acta Tropica |
| Volume | 118 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 2011 |
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Keywords
- Anopheles
- ITS2
- Malaria
- PCR-RFLP
- Species identification
ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
Cite this
Evaluation of a PCR-RFLP-ITS2 assay for discrimination of Anopheles species in northern and western Colombia. / Cienfuegos, Astrid V.; Rosero, Doris A.; Naranjo, Nelson; Luckhart, Shirley; Conn, Jan E.; Correa, Margarita M.
In: Acta Tropica, Vol. 118, No. 2, 05.2011, p. 128-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of a PCR-RFLP-ITS2 assay for discrimination of Anopheles species in northern and western Colombia
AU - Cienfuegos, Astrid V.
AU - Rosero, Doris A.
AU - Naranjo, Nelson
AU - Luckhart, Shirley
AU - Conn, Jan E.
AU - Correa, Margarita M.
PY - 2011/5
Y1 - 2011/5
N2 - Anopheles mosquitoes are routinely identified using morphological characters of the female that often lead to misidentification due to interspecies similarity and intraspecies variability. The aim of this work was to evaluate the applicability of a previously developed PCR-RFLP-ITS2 assay for accurate discrimination of anophelines in twelve localities spanning three Colombian malaria epidemiological regions: Atlantic Coast, Pacific Coast, and Uraba-Bajo Cauca-Alto Sinu region. The evaluation of the stability of the PCR-RFLP patterns is required since variability of the ITS2 has been documented and may produce discrepancies in the patterns previously reported. The assay was used to evaluate species assignation of 939 mosquitoes identified by morphology. Strong agreement between the morphological and molecular identification was found for species Anopheles albimanus, Anopheles aquasalis, Anopheles darlingi and Anopheles triannulatus s.l. (p≥0.05, kappa. =1). However, disagreement was found for species Anopheles nuneztovari s.l., Anopheles neomaculipalpus, Anopheles apicimacula and Anopheles punctimacula (p≤0.05; kappa ranging from 0.33 to 0.80). The ITS2-PCR-RFLP assay proved valuable for discriminating anopheline species of northern and western Colombia, especially those with overlapping morphology in the Oswaldoi Group.
AB - Anopheles mosquitoes are routinely identified using morphological characters of the female that often lead to misidentification due to interspecies similarity and intraspecies variability. The aim of this work was to evaluate the applicability of a previously developed PCR-RFLP-ITS2 assay for accurate discrimination of anophelines in twelve localities spanning three Colombian malaria epidemiological regions: Atlantic Coast, Pacific Coast, and Uraba-Bajo Cauca-Alto Sinu region. The evaluation of the stability of the PCR-RFLP patterns is required since variability of the ITS2 has been documented and may produce discrepancies in the patterns previously reported. The assay was used to evaluate species assignation of 939 mosquitoes identified by morphology. Strong agreement between the morphological and molecular identification was found for species Anopheles albimanus, Anopheles aquasalis, Anopheles darlingi and Anopheles triannulatus s.l. (p≥0.05, kappa. =1). However, disagreement was found for species Anopheles nuneztovari s.l., Anopheles neomaculipalpus, Anopheles apicimacula and Anopheles punctimacula (p≤0.05; kappa ranging from 0.33 to 0.80). The ITS2-PCR-RFLP assay proved valuable for discriminating anopheline species of northern and western Colombia, especially those with overlapping morphology in the Oswaldoi Group.
KW - Anopheles
KW - ITS2
KW - Malaria
KW - PCR-RFLP
KW - Species identification
UR - http://www.scopus.com/inward/record.url?scp=79955476123&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955476123&partnerID=8YFLogxK
U2 - 10.1016/j.actatropica.2011.02.004
DO - 10.1016/j.actatropica.2011.02.004
M3 - Article
C2 - 21345325
AN - SCOPUS:79955476123
VL - 118
SP - 128
EP - 135
JO - Acta Tropica
JF - Acta Tropica
SN - 0001-706X
IS - 2
ER -