Evaluation of a digitonin disk assay to discriminate between acholeplasma and mycoplasma isolates from bovine milk.

Mark Thurmond, C. A. Holmberg, D. M. Luiz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A study was undertaken to evaluate effectiveness of a digitonin disk inhibition test to discriminate between Acholeplasma laidlawii and Mycoplasma sp. isolated from bovine milk. The test measured zone diameters of growth inhibition surrounding a digitonin-containing disk on solid medium. Zones of inhibition for 20 isolates of A. laidlawii, ranging from 8-14 mm, did not overlap those of 261 isolates of Mycoplasma sp., ranging from 16 to 38 mm. Examination of variation in zone diameters for M. bovis found that inhibition was not appreciably affected by agar dehydration. Zones of inhibition increased with increasing dilutions of stock culture and decreased with increasing incubation time. Analysis of variance and Fisher's least significant difference test of logn zone diameters revealed that differences in mean logn zone diameters were different at the 0.01 level of significance between some of the six species of mycoplasma examined, indicating that growth among some species of mycoplasma was effected differently by digitonin. The digitonin test was found to clearly discriminate between A. laidlawii and Mycoplasma sp. indicating that the test would be useful as a practical screening test of individual-cow and bulk tank milk for mycoplasmas.

Original languageEnglish (US)
Pages (from-to)71-81
Number of pages11
JournalThe Cornell veterinarian
Volume79
Issue number1
StatePublished - Jan 1 1989

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Acholeplasma
Digitonin
Mycoplasma
Milk
Acholeplasma laidlawii
Growth
Dehydration
Agar
Analysis of Variance

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Evaluation of a digitonin disk assay to discriminate between acholeplasma and mycoplasma isolates from bovine milk. / Thurmond, Mark; Holmberg, C. A.; Luiz, D. M.

In: The Cornell veterinarian, Vol. 79, No. 1, 01.01.1989, p. 71-81.

Research output: Contribution to journalArticle

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