Evaluation of a collagenase generated osteoarthritis biomarker in the synovial fluid from elbow joints of dogs with medial coronoid disease and unaffected dogs

Adam Prink, Kei Hayashi, Sun Young Kim, James Kim, Amy Kapatkin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective To evaluate whether synovial fluid concentrations of an osteoarthritis biomarker in dysplastic canine elbows with medial coronoid disease (MCD) are elevated compared with unaffected elbows and to determine if these concentrations correlate to the degree of articular cartilage damage. Study Design Cross sectional clinical study. Animals Dogs (n=19; 35 elbows) with MCD and dogs (8; 16 elbows) with unaffected elbows. Methods Concentrations of a collagenase-generated cleavage neoepitope of type II collagen (Col2-3/4C long mono, or C2C) in joint fluid from elbows were analyzed and compared between dogs with MCD and unaffected dogs. Correlation of C2C concentration with subjective grading of articular cartilage surface damage was also evaluated. Results Mean (±SD) C2C concentration from MCD dogs was significantly higher (112.3±24.8 ng/mL) than in unaffected dogs (76.1±16.9 ng/mL; P<.05). There was a moderate correlation between cartilage damage grade and increasing C2C concentrations (P<.05, r=0.62) Conclusion C2C concentrations are elevated in the synovial fluid of dogs with MCD compared with unaffected elbows, and a moderate, significant correlation was identified between these concentrations and subjective grading of articular cartilage damage. Clinical Relevance This preliminary data suggest that C2C concentrations in synovial fluid may have potential as a biomarker for diagnosis of articular cartilage damage associated with MCD and as a means of objectively determining the degree of articular cartilage damage.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalVeterinary Surgery
Volume39
Issue number1
DOIs
StatePublished - Jan 2010

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Dog Diseases
Elbow Joint
synovial fluid
collagenase
elbows
osteoarthritis
Synovial Fluid
Collagenases
Elbow
joints (animal)
Osteoarthritis
Articular Cartilage
biomarkers
cartilage
Biomarkers
Dogs
dogs
Collagen Type II
dog diseases
Cartilage

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of a collagenase generated osteoarthritis biomarker in the synovial fluid from elbow joints of dogs with medial coronoid disease and unaffected dogs. / Prink, Adam; Hayashi, Kei; Kim, Sun Young; Kim, James; Kapatkin, Amy.

In: Veterinary Surgery, Vol. 39, No. 1, 01.2010, p. 65-70.

Research output: Contribution to journalArticle

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abstract = "Objective To evaluate whether synovial fluid concentrations of an osteoarthritis biomarker in dysplastic canine elbows with medial coronoid disease (MCD) are elevated compared with unaffected elbows and to determine if these concentrations correlate to the degree of articular cartilage damage. Study Design Cross sectional clinical study. Animals Dogs (n=19; 35 elbows) with MCD and dogs (8; 16 elbows) with unaffected elbows. Methods Concentrations of a collagenase-generated cleavage neoepitope of type II collagen (Col2-3/4C long mono, or C2C) in joint fluid from elbows were analyzed and compared between dogs with MCD and unaffected dogs. Correlation of C2C concentration with subjective grading of articular cartilage surface damage was also evaluated. Results Mean (±SD) C2C concentration from MCD dogs was significantly higher (112.3±24.8 ng/mL) than in unaffected dogs (76.1±16.9 ng/mL; P<.05). There was a moderate correlation between cartilage damage grade and increasing C2C concentrations (P<.05, r=0.62) Conclusion C2C concentrations are elevated in the synovial fluid of dogs with MCD compared with unaffected elbows, and a moderate, significant correlation was identified between these concentrations and subjective grading of articular cartilage damage. Clinical Relevance This preliminary data suggest that C2C concentrations in synovial fluid may have potential as a biomarker for diagnosis of articular cartilage damage associated with MCD and as a means of objectively determining the degree of articular cartilage damage.",
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