Evaluation of a blocking enzyme-linked immunosorbent assay for serological diagnosis of Bovine herpesvirus 1

Jennifer A. Godhardt-Cooper, Jeanne Zoromski, Kathy L. Kurth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bovine herpesvirus 1 (BoHV-1) is an infectious agent of concern in the international export of bovine products; it is endemic in the United States, but it has been eradicated in many countries of the European Union (EU). For export of semen to the EU, accurate assessment of BoHV-1 status of the bull is required and is usually accomplished by measuring the level of antibody to the virus. The gold standard is virus neutralization (VN) using overnight incubation with the virus, a test approved by the World Organization for Animal Health (OIE). Enzyme-linked immunosorbent assay (ELISA) is also approved for international trade. The lone U.S. Department of Agriculture-approved commercial ELISA was compromised with specificity problems, which necessitated the development of a different ELISA. Of 4 monoclonal antibodies evaluated, 1 directed against glycoprotein C of BoHV-1 was found to be the most reliable. One hundred twenty-eight characterized positive samples and 334 negative serum samples were tested. The blocking ELISA showed 97.7% sensitivity and 99.4% specificity as compared with OIE VN. The Wisconsin Veterinary Diagnostic Laboratory ELISA fulfills the OIE requirement for a blocking or competitive ELISA to qualify animals for export to BoHV-1-free countries.

Original languageEnglish (US)
Pages (from-to)523-526
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume21
Issue number4
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint

Bovine Herpesvirus 1
Bovine herpesvirus 1
serodiagnosis
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Viruses
neutralization tests
European Union
United States Department of Agriculture
viruses
international trade
Semen
animal health
USDA
gold
glycoproteins
semen
monoclonal antibodies
bulls
Monoclonal Antibodies

Keywords

  • Bovine herpesvirus 1
  • G2 antibody
  • Glycoprotein C
  • Infectious bovine rhinotracheitis
  • Virus neutralization

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of a blocking enzyme-linked immunosorbent assay for serological diagnosis of Bovine herpesvirus 1. / Godhardt-Cooper, Jennifer A.; Zoromski, Jeanne; Kurth, Kathy L.

In: Journal of Veterinary Diagnostic Investigation, Vol. 21, No. 4, 01.01.2009, p. 523-526.

Research output: Contribution to journalArticle

@article{f9e648c317794e43b12ed3355c135bb6,
title = "Evaluation of a blocking enzyme-linked immunosorbent assay for serological diagnosis of Bovine herpesvirus 1",
abstract = "Bovine herpesvirus 1 (BoHV-1) is an infectious agent of concern in the international export of bovine products; it is endemic in the United States, but it has been eradicated in many countries of the European Union (EU). For export of semen to the EU, accurate assessment of BoHV-1 status of the bull is required and is usually accomplished by measuring the level of antibody to the virus. The gold standard is virus neutralization (VN) using overnight incubation with the virus, a test approved by the World Organization for Animal Health (OIE). Enzyme-linked immunosorbent assay (ELISA) is also approved for international trade. The lone U.S. Department of Agriculture-approved commercial ELISA was compromised with specificity problems, which necessitated the development of a different ELISA. Of 4 monoclonal antibodies evaluated, 1 directed against glycoprotein C of BoHV-1 was found to be the most reliable. One hundred twenty-eight characterized positive samples and 334 negative serum samples were tested. The blocking ELISA showed 97.7{\%} sensitivity and 99.4{\%} specificity as compared with OIE VN. The Wisconsin Veterinary Diagnostic Laboratory ELISA fulfills the OIE requirement for a blocking or competitive ELISA to qualify animals for export to BoHV-1-free countries.",
keywords = "Bovine herpesvirus 1, G2 antibody, Glycoprotein C, Infectious bovine rhinotracheitis, Virus neutralization",
author = "Godhardt-Cooper, {Jennifer A.} and Jeanne Zoromski and Kurth, {Kathy L.}",
year = "2009",
month = "1",
day = "1",
doi = "10.1177/104063870902100416",
language = "English (US)",
volume = "21",
pages = "523--526",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "4",

}

TY - JOUR

T1 - Evaluation of a blocking enzyme-linked immunosorbent assay for serological diagnosis of Bovine herpesvirus 1

AU - Godhardt-Cooper, Jennifer A.

AU - Zoromski, Jeanne

AU - Kurth, Kathy L.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Bovine herpesvirus 1 (BoHV-1) is an infectious agent of concern in the international export of bovine products; it is endemic in the United States, but it has been eradicated in many countries of the European Union (EU). For export of semen to the EU, accurate assessment of BoHV-1 status of the bull is required and is usually accomplished by measuring the level of antibody to the virus. The gold standard is virus neutralization (VN) using overnight incubation with the virus, a test approved by the World Organization for Animal Health (OIE). Enzyme-linked immunosorbent assay (ELISA) is also approved for international trade. The lone U.S. Department of Agriculture-approved commercial ELISA was compromised with specificity problems, which necessitated the development of a different ELISA. Of 4 monoclonal antibodies evaluated, 1 directed against glycoprotein C of BoHV-1 was found to be the most reliable. One hundred twenty-eight characterized positive samples and 334 negative serum samples were tested. The blocking ELISA showed 97.7% sensitivity and 99.4% specificity as compared with OIE VN. The Wisconsin Veterinary Diagnostic Laboratory ELISA fulfills the OIE requirement for a blocking or competitive ELISA to qualify animals for export to BoHV-1-free countries.

AB - Bovine herpesvirus 1 (BoHV-1) is an infectious agent of concern in the international export of bovine products; it is endemic in the United States, but it has been eradicated in many countries of the European Union (EU). For export of semen to the EU, accurate assessment of BoHV-1 status of the bull is required and is usually accomplished by measuring the level of antibody to the virus. The gold standard is virus neutralization (VN) using overnight incubation with the virus, a test approved by the World Organization for Animal Health (OIE). Enzyme-linked immunosorbent assay (ELISA) is also approved for international trade. The lone U.S. Department of Agriculture-approved commercial ELISA was compromised with specificity problems, which necessitated the development of a different ELISA. Of 4 monoclonal antibodies evaluated, 1 directed against glycoprotein C of BoHV-1 was found to be the most reliable. One hundred twenty-eight characterized positive samples and 334 negative serum samples were tested. The blocking ELISA showed 97.7% sensitivity and 99.4% specificity as compared with OIE VN. The Wisconsin Veterinary Diagnostic Laboratory ELISA fulfills the OIE requirement for a blocking or competitive ELISA to qualify animals for export to BoHV-1-free countries.

KW - Bovine herpesvirus 1

KW - G2 antibody

KW - Glycoprotein C

KW - Infectious bovine rhinotracheitis

KW - Virus neutralization

UR - http://www.scopus.com/inward/record.url?scp=67849126824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67849126824&partnerID=8YFLogxK

U2 - 10.1177/104063870902100416

DO - 10.1177/104063870902100416

M3 - Article

C2 - 19564503

AN - SCOPUS:67849126824

VL - 21

SP - 523

EP - 526

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 4

ER -