Evaluation and comparison of an indirect fluorescent antibody test for detection of antibodies to Sarcocystis neurona, using serum and cerebrospinal fluid of naturally and experimentally infected, and vaccinated horses

Paulo C. Duarte, Barbara M. Daft, Patricia A Conrad, Andrea E. Packham, William J. Saville, Robert J. MacKay, Bradd C. Barr, William D Wilson, Terry Ng, Stephen M. Reed, Ian Gardner

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Abstract

The objectives of this study were to evaluate the accuracy of the indirect fluorescent antibody test (IFAT) using serum and cerebrospinal fluid (CSF) of horses naturally and experimentally infected with Sarcocystis neurona, to assess the correlation between serum and CSF titers, and to determine the effect of S. neurona vaccination on the diagnosis of infection. Using receiver-operating characteristic analysis, the areas under the curve for the IFAT were 0.97 (serum) and 0.99 (CSF). Sensitivity and specificity were 83.3 and 96.9% (serum, cutoff 80) and 100 and 99% (CSF cutoff 5), respectively. Titer-specific likelihood ratios (LRs) ranged from 0.03 to 187.8 for titers between <10 and 640. Median time to conversion was 22-26 days postinfection (DPI) (serum) and 30 DPI (CSF). The correlation between serum and CSF titers was moderately strong (r = 0.6) at 30 DPI. Percentage of vaccinated antibody-positive horses ranged from 0 to 95% between 0 and 112 days after the second vaccination. Thus, the IFAT was reliable and accurate using serum and CSF Use of LRs potentially improves clinical decision making. Correlation between serum and CSF titers affects the joint accuracy of the IFAT; therefore, the ratio of serum to CSF titers has potential diagnostic value. The S. neurona vaccine could possibly interfere with equine protozoal myeloencephalitis diagnosis.

Original languageEnglish (US)
Pages (from-to)379-386
Number of pages8
JournalJournal of Parasitology
Volume90
Issue number2
DOIs
StatePublished - Apr 2004

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Sarcocystis
Sarcocystis neurona
cerebrospinal fluid
antibody detection
horse
Horses
antibody
Cerebrospinal Fluid
serum
horses
antibodies
fluid
Antibodies
Serum
testing
vaccination
Vaccination
equine protozoal myeloencephalitis
Encephalomyelitis
test

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Evaluation and comparison of an indirect fluorescent antibody test for detection of antibodies to Sarcocystis neurona, using serum and cerebrospinal fluid of naturally and experimentally infected, and vaccinated horses. / Duarte, Paulo C.; Daft, Barbara M.; Conrad, Patricia A; Packham, Andrea E.; Saville, William J.; MacKay, Robert J.; Barr, Bradd C.; Wilson, William D; Ng, Terry; Reed, Stephen M.; Gardner, Ian.

In: Journal of Parasitology, Vol. 90, No. 2, 04.2004, p. 379-386.

Research output: Contribution to journalArticle

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