Evaluating the relationship between plasma and skin carotenoids and reported dietary intake in elementary school children to assess fruit and vegetable intake

Lori M. Nguyen, Rachel E. Scherr, Jessica D. Linnell, Igor V. Ermakov, Werner Gellermann, Lisa Jahns, Carl L Keen, Sheridan Miyamoto, Francene M. Steinberg, Heather M Young, Sheri Zidenberg-Cherr

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Accurate assessment of dietary intake of children can be challenging due to the limited reliability of current dietary assessment methods. Plasma carotenoid concentration has been used to assess fruit and vegetable intake, but this testing is rarely conducted in school settings in children. Resonance Raman spectroscopy (RRS) is emerging as a useful method to objectively assess fruit and vegetable intake. This methodology has been validated in adults, but limited work has been done in children, particularly in the school setting. The purpose of this research is to further validate the RRS methodology in children. Children (9-12 year) participating in a school-based intervention were recruited. Plasma carotenoids were quantified using HPLC, skin carotenoid status was measured using RRS, and dietary intake of carotenoids was measured with the Block Food Frequency Questionnaire Ages 8-17. Total plasma carotenoid concentrations and skin carotenoid intensities were strongly correlated (r=0.62, p<0.001, n=38). Reported total carotenoid intake correlated with skin carotenoids (r=0.40, p<0.0001, n=128). Skin carotenoid status as measured by RRS can be a strong predictor of plasma carotenoid status and dietary intake of carotenoids in children. RRS may be used as a valid, non-invasive, and useful method to assess fruit and vegetable intakes in this population.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume572
DOIs
StatePublished - Apr 15 2015

Fingerprint

Vegetables
Carotenoids
Fruits
Fruit
Skin
Plasmas
Raman Spectrum Analysis
Raman spectroscopy
High Pressure Liquid Chromatography
Food

Keywords

  • Biomarkers
  • Carotenoids
  • Dietary assessment
  • Raman spectroscopy
  • Validation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Evaluating the relationship between plasma and skin carotenoids and reported dietary intake in elementary school children to assess fruit and vegetable intake. / Nguyen, Lori M.; Scherr, Rachel E.; Linnell, Jessica D.; Ermakov, Igor V.; Gellermann, Werner; Jahns, Lisa; Keen, Carl L; Miyamoto, Sheridan; Steinberg, Francene M.; Young, Heather M; Zidenberg-Cherr, Sheri.

In: Archives of Biochemistry and Biophysics, Vol. 572, 15.04.2015, p. 73-80.

Research output: Contribution to journalArticle

Nguyen, LM, Scherr, RE, Linnell, JD, Ermakov, IV, Gellermann, W, Jahns, L, Keen, CL, Miyamoto, S, Steinberg, FM, Young, HM & Zidenberg-Cherr, S 2015, 'Evaluating the relationship between plasma and skin carotenoids and reported dietary intake in elementary school children to assess fruit and vegetable intake', Archives of Biochemistry and Biophysics, vol. 572, pp. 73-80. https://doi.org/10.1016/j.abb.2015.02.015
Nguyen, Lori M. ; Scherr, Rachel E. ; Linnell, Jessica D. ; Ermakov, Igor V. ; Gellermann, Werner ; Jahns, Lisa ; Keen, Carl L ; Miyamoto, Sheridan ; Steinberg, Francene M. ; Young, Heather M ; Zidenberg-Cherr, Sheri. / Evaluating the relationship between plasma and skin carotenoids and reported dietary intake in elementary school children to assess fruit and vegetable intake. In: Archives of Biochemistry and Biophysics. 2015 ; Vol. 572. pp. 73-80.
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